Overview:
| Product Name | RYK Antibody | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Description | Rabbit Anti-Human RYK Polyclonal | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Species Reactivity | Mouse, Rat | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Applications | WB | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Antibody Dilution | WB (1:1000); optimal dilutions for assays should be determined by the user. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Host Species | Rabbit | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Immunogen Species | Human | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Immunogen | Synthetic peptide of Human RYK (150-250 aa), conjugated to Keyhole Limpet Haemocyanin (KLH). | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Concentration | 1 mg/ml | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Conjugates |
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
StreptavidinProperties:
Streptavidin Datasheet Biotin | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| R-PE (R-Phycoerythrin) | ||
Overview:
R-PE Datasheet | ![]() | Optical Properties: λex = 565 nm λem = 575 nm εmax = 2.0×106 Φf = 0.84 Brightness = 1.68 x 103 Laser = 488 to 561 nm Filter set = TRITC |
Properties
| Storage Buffer | PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide |
| Storage Temperature | -20ºC |
| Shipping Temperature | Blue Ice or 4ºC |
| Purification | Peptide Affinity Purified |
| Clonality | Polyclonal |
| Specificity | Detects 67.5 kDa. Band at 37 kDa is potential degradation product. . |
| Cite This Product | StressMarq Biosciences Cat# SPC-740, RRID: AB_2706491 |
| Certificate of Analysis | A 1:1000 dilution of SPC-740 was sufficient for detection of RYK in 15 µg of mouse brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. |
Biological Description
| Alternative Names | Tyrosine protein kinase RYK precursor Antibody, D3S3195 Antibody, Vik Antibody, Tyrosine protein kinase RYK Antibody, JTK5A protein tyrosine kinase Antibody, RYK1 Antibody, RYK 1 Antibody, receptor like tyrosine kinase precursor Antibody, Tyrosine-protein kinase RYK Antibody, receptor like tyrosine kinase Antibody, ERK 3 Antibody, RYK Antibody, JTK 5A Antibody, Hydroxyaryl protein kinase Antibody, JTK5 Antibody, JTK5A Antibody, RYK_HUMAN Antibody, Ryk Antibody, JTK 5 Antibody |
| Research Areas | Bone, Cell Signaling, Extracellular Matrix (ECM), Mesenchymal Stem Cells, Osteogenesis, Protein Phosphorylation, Receptor Tyrosine Kinases, Signaling Pathways, Stem Cells, Tyrosine Kinases, Wnt / Beta Catenin Pathway |
| Cellular Localization | Cytoplasm, Membrane, Nucleus, Single-Pass Type I Membrane Protein |
| Accession Number | NP_001005861.1 |
| Gene ID | 6259 |
| Swiss Prot | P34925 |
Product Images
Western blot analysis of Mouse brain lysate showing detection of ~67.5 kDa RYK protein using Rabbit Anti-RYK Polyclonal Antibody (SPC-740). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain lysate. Load: 15 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Rabbit Anti-RYK Polyclonal Antibody (SPC-740) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~67.5 kDa. Other Band(s): ~37 kDa degradation product.
Product Citations (0)
Currently there are no citations for this product.
| ATTO 488 | ||
Overview:
ATTO 488 Datasheet | ![]() | Optical Properties: λex = 501 nm λem = 523 nm εmax = 9.0×104 Φf = 0.80 τfl = 4.1 ns Brightness = 72 Laser = 488 nm Filter set = FITC |
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常用流动相加酸碱后PH的总结,希望大家能够提供一点自己测过的结果,谢谢先
1.直接用固体磷酸钠配制成50mM的磷酸钠溶液,再调pH到7.4;(我们试着用这个做了下,发现挂不上柱)
2.配置磷酸钠盐缓冲液:按NaH2PO4:Na2HPO4以19:81的摩尔比配制成pH7.4的缓冲液?(附一张百度出来的配方
)
3.如果是磷酸钠盐缓冲液,可以直接将50mM的NaH2PO4的水溶液用NaOH调成pH7.4吗?
再者,2和3这两个方法配制的磷酸钠盐缓冲液有什么区别?最终效果是一样的吗?如果不一样,有什么理论的知识支撑呢?个人感觉是分析化学中酸碱理论中的缓冲液那里的知识。求帮忙解答这些疑问。
另外,我还想问一下,pH对于Ni柱对His-tagged的蛋白的分离纯化影响大吗?是怎么影响的?谢谢大家了!
有了源数据之后把源数据按照大小排列,
选中源数据区域-->ALT+A1-->选中图标区右键-->更改图表类型-->散点图
因为是考察不同PH对药物的影响,样品又不好改变其PH值,这种情况怎么办?希望有经验的高手指教。
我的流动相是甲醇-水(90:10)
谢谢赐教!
请进子版按格式发贴,自行修改,谢谢。
由弱酸及其盐、弱碱及其盐组成的混合溶液,能在一定程度上抵消、减轻外加强酸或强碱对溶液酸碱度的影响,从而保持溶液的pH值相对稳定。这种溶液称为缓冲溶液。
:)
我在做一细菌不同酸碱度生长状况时,发现这些奇怪现象:pH=3的培养基灭菌(TSB液体培养基)灭菌后pH上升到到9.2!而原来pH=9.0的降到8.7(基本没多少变化),请问各位大侠,这是什么原因?
一般做不同酸碱度生长实验时,该如何才能防止pH在湿热灭菌后基本不变化?
是否可以理解为纯化水得PH范围为6.3-7.6?能否直接用pH计测量?谢谢!















