Overview:
| Product Name | Survivin Antibody | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Description | Rabbit Anti-Human Survivin Polyclonal | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Species Reactivity | Human, Mouse, Rat | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Applications | WB, IHC, ICC/IF | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Antibody Dilution | WB (1:2000), IHC (1:200), ICC/IF (1:500); optimal dilutions for assays should be determined by the user. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Host Species | Rabbit | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Immunogen Species | Human | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Immunogen | A synthesized peptide derived from human Survivin | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Conjugates |
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
StreptavidinProperties:
Streptavidin Datasheet Biotin | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| R-PE (R-Phycoerythrin) | ||
Overview:
R-PE Datasheet | ![]() | Optical Properties: λex = 565 nm λem = 575 nm εmax = 2.0×106 Φf = 0.84 Brightness = 1.68 x 103 Laser = 488 to 561 nm Filter set = TRITC |
Properties
| Storage Buffer | PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide |
| Storage Temperature | -20ºC |
| Shipping Temperature | Blue Ice or 4ºC |
| Purification | Affinity Purified |
| Clonality | Polyclonal |
| Isotype | IgG |
| Specificity | Detects ~16kDa, endogenous levels of total Survivin |
| Cite This Product | StressMarq Biosciences Cat# SPC-1335, RRID: AB_2713817 |
| Certificate of Analysis | A 1:1000 dilution of SPC-1335 was sufficient for detection of Survivin in 10 µg of Lung cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody. |
Biological Description
| Alternative Names | API4 Antibody, Apoptosis inhibitor 4 Antibody, Apoptosis inhibitor survivin Antibody, Baculoviral IAP repeat containing 5 Antibody, BIRC 5 Antibody, EPR 1 Antibody, IAP4 Antibody, Survivin variant 3 alpha Antibody, SVV Antibody, TIAP Antibody |
| Research Areas | Cancer, Apoptosis, Neurodegeneration, Neuroscience |
| Cellular Localization | Cytoplasm, Chromosome, Nucleus |
| Accession Number | NP_001159.2 |
| Gene ID | 332 |
| Swiss Prot | O15392 |
| Scientific Background | Survivin, or baculovirus inhibitor of apoptosis repeat-containing 5 is a member of the inhibitor of apoptosis family (IAP). It functions to inhibit caspase activation. It may also play a role in regulating mitosis. It is regulated by the p53 protein, a direct target gene of the Wnt pathway, and is upregulated by beta-catenin. |
| References |
1. Altieri D.C. (1994). J. Biol. Chem. 269(5): 3139–3142. 2. Sah N.K., Khan Z., Khan G.J., Bisen P.S. (2006). Cancer Lett. 244(2): 164–171. 3. Olie R.A., et al. (2000). Cancer Res. 60(11): 2805–2809. |
Product Images
Western blot analysis of Mouse Lung cell lysates showing detection of ~20kDa Survivin protein using Rabbit Anti-Survivin Polyclonal Antibody (SPC-1335). Lane 1: Mouse Lung tissue lysate treated with the immunizing peptide. Lane 2: Mouse Lung tissue lysate. Primary Antibody: Rabbit Anti-Survivin Polyclonal Antibody (SPC-1335) at 1:1000. Predicted/Observed Size: ~20kDa.
Product Citations (0)
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| ATTO 488 | ||
Overview:
ATTO 488 Datasheet | ![]() | Optical Properties: λex = 501 nm λem = 523 nm εmax = 9.0×104 Φf = 0.80 τfl = 4.1 ns Brightness = 72 Laser = 488 nm Filter set = FITC |
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常用流动相加酸碱后PH的总结,希望大家能够提供一点自己测过的结果,谢谢先
1.直接用固体磷酸钠配制成50mM的磷酸钠溶液,再调pH到7.4;(我们试着用这个做了下,发现挂不上柱)
2.配置磷酸钠盐缓冲液:按NaH2PO4:Na2HPO4以19:81的摩尔比配制成pH7.4的缓冲液?(附一张百度出来的配方
)
3.如果是磷酸钠盐缓冲液,可以直接将50mM的NaH2PO4的水溶液用NaOH调成pH7.4吗?
再者,2和3这两个方法配制的磷酸钠盐缓冲液有什么区别?最终效果是一样的吗?如果不一样,有什么理论的知识支撑呢?个人感觉是分析化学中酸碱理论中的缓冲液那里的知识。求帮忙解答这些疑问。
另外,我还想问一下,pH对于Ni柱对His-tagged的蛋白的分离纯化影响大吗?是怎么影响的?谢谢大家了!
有了源数据之后把源数据按照大小排列,
选中源数据区域-->ALT+A1-->选中图标区右键-->更改图表类型-->散点图
因为是考察不同PH对药物的影响,样品又不好改变其PH值,这种情况怎么办?希望有经验的高手指教。
我的流动相是甲醇-水(90:10)
谢谢赐教!
请进子版按格式发贴,自行修改,谢谢。
由弱酸及其盐、弱碱及其盐组成的混合溶液,能在一定程度上抵消、减轻外加强酸或强碱对溶液酸碱度的影响,从而保持溶液的pH值相对稳定。这种溶液称为缓冲溶液。
:)
我在做一细菌不同酸碱度生长状况时,发现这些奇怪现象:pH=3的培养基灭菌(TSB液体培养基)灭菌后pH上升到到9.2!而原来pH=9.0的降到8.7(基本没多少变化),请问各位大侠,这是什么原因?
一般做不同酸碱度生长实验时,该如何才能防止pH在湿热灭菌后基本不变化?
是否可以理解为纯化水得PH范围为6.3-7.6?能否直接用pH计测量?谢谢!















