
Type:BlockingBuffer
Applications:E
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysFormat:liquid
NeptuneBlockcontainsaproprietarynon-mammalianformulationbasedonfishproteins,plusmolecularstABIlizersthatprovideahighdegreeofblockingefficiency.NeptuneBlockwasdesignedforantigen-downELISAformats,andforsandwichassayswithhighbackgroundproblems.
Thisformulationisaheterogeneousmixtureofsmallmoleculescapableofblockingboththenonspecificbindingsitesoftheadsorbedprotein,aswellastheunoccupiedregionsofthepolystyreneplatethatarenotstericallyaccessIBLetolargertrADItionalmammalianblockingreagents,therebyreducingbackgroundnoise.Thesmallsizeoftheseuniqueblockersresultsinminimalsterichindrancetokeyepitoperegionsoftargetcoatedproteins,thusenhancingspecificsignalgeneration.
AsNeptuneBlockcontainsanon-mammalianproteinblockingagent,itisantigenicityforeigntomostmammalianimmunesystems.Inantigen-downELISAformats,thisreducesthepossibilityoffalse-positivesgeneratedfromendogenousantibodiesinthesamplereactingwithplateblockingproteins.NeptuneBlockisparticularlyusefulwhenworkingwithhuman,swine,andbovineserumsamples,asthereislittleinteractionwiththeblockingmoleculesresultinginlowerbackgrounds.
RecommendedUses:
- Antigen-downELISAs.
- SandwichELISAswithhighbackgrounds.
- ELISAstestinghumanandothermammaliansamples
ebiomall.com






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加样缓冲液的主要作用是使PCR产物与其混合,
使DNA沉于加样孔的底部,防止DNA跑出来.
蛋白质上样缓冲液中的SDS和DTT分别可以屏蔽蛋白质电荷以及破坏二硫键,避免形成多聚体。
DNA上样缓冲液主要是起沉降和指示作用,不可用于蛋白,否则会影响蛋白相对分子量定量。
同样蛋白上样缓冲液中的tris-HCl浓度过高也会使溴酚蓝条带扭曲。

