Wheaton Lexan Polycarbonate wide mouth polycarbonate bottles are engineered for applications including ultracentrifugation or culture of microbes, plants, algae, and organisms requiring large surface area to volume ratios. As ultracentrifuge flasks, they withstand maximum speeds without leakage and are ideal for all GS3 rotors. Centrifuge bottles are used where relatively small amounts of solids are involved. The tight- fitting polypropylene screw cap reduces the possibility of aerosol escape.
Wheaton Lexan Polycarbonate resin is an amorphous engineering thermoplastic, characterized by outstanding mechanical, optical, electrical, and thermal properties. The Lexan resins employed are environmentally conforming to flame retardancy, scratch resistance, toughness, heat resistance, weatherability, biocompatibility, optical quality, and compliance with stringent FDA and USP requirements.
These vessels are supplied aseptically clean and nonsterile. They are packaged and produced in a controlled cleanroom environment and do not require rinsing prior to first use. As such, this polycarbonate labware is a safe alternative to glass. Bottles are ideal for steam sterilization of all biologicals, liquids, reagents, media, buffers, and aqueous solutions.They withstand repeated autoclaving, and are optimal for all microbial culture media production and preparation of terminally sterile fluids.
| SKU | W017780 |
|---|---|
| Color | CLEAR |
| Capacity(mL) | 2000 |
| Pyrogen Free? | Yes |
| DNAse, RNAse Free | Yes |
| Cap Color | Blue |
| Bottom Style | Baffled |
| Plastic Material | Polycarbonate |
| Cap Material | Polypropylene |
| Sterile | Yes |
| Cap/ Closure Size | 38-430 |
| Bottle Style | Erlenmeyer |
| Bottle Material | Plastic |
| Closure Style | Vented DuoCap |
Product Literature
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最好是测浓度，一般不超过30-40ug，上样缓冲液一般4×或者5×，这个没太大区别。
1。称量氨基丁三醇 242g，Na2EDTA.2H2O 37.2g 于1L烧杯中；
2。向烧杯中加入约600ml去离子水，充分搅拌均匀；
3。加入57.1ml的冰乙酸，充分溶解；
4。用NaOH调pH至8.3，加去离子水定容至1L后，室温保存。
使用时稀释50倍 即1×TAE Buffer
10×TBE Buffer配制方法：
1。称量氨基丁三醇 108g，Na2EDTA.2H2O 7.44g，硼酸55g 于1L烧杯中；
2。加入约700ml去离子水，搅拌均匀；
3。用NaOH调pH至8.3，加去离子水定容至1L后，室温保存。
使用时稀释10倍 即1×TBE Buffer
2乘SDS-PAGE上样缓冲液（20ml体系）
1.0mol/L Tris-HCL (pH 6.8) 2ml
1.0mol/L DTT 4ml
SDS 0.8g
溴芬蓝 0.04g
甘油 4ml
dd水 定容至20ml
4℃冰箱保存，可以不分装，我们实验室一般用三个月以上
先配1.0mol/L的DTT,双蒸无菌水溶解4℃冰箱保存,配上样缓冲液的时候加入就可以了,都混匀保存也没问题的
配制分三步： 1) 1 M Tris-HCl (pH 8.0) 50 ml的配制：称取Tris碱6.06 g，加超纯水40 ml溶解，滴加浓HCl约2.1 ml调pH至8.0，定容至50 ml。
① 上样缓冲液中包括甘油,溴酚蓝,SDS等,甘油主要作用是防止样品漂浮出点样孔,溴酚蓝作为电泳指示剂,用来观察电泳进度,SDS用于一些核酸结合蛋白的变性解离.
②核酸染料与凝胶中核酸结合,以便于在紫外光下显示核酸条带.
