- Overview
- Data/Specifications
- Literature/Support
- How To Use
- Related Products
Overview
Type I collagen is the most abundant collagen and is found in connective tissues including tendon, ligament, dermis and blood vessel. It is the major component and the primary determinant of tensile strength of the extracelluar matrix (ECM). It is widely used as a thin layer on tissue-culture surfaces to enhance the attachment and proliferation of a variety of cells including endothelial cells, fibroblasts, hepatocytes, epithelial cells etc. In addition, collagen I can self-assemble into a 3-D superamolecular gel in vitro, making it an ideal biological scaffold to promote more in vivo-like cellular morphology and function.
Symbols/Related Terms:
- COL1A1
- COL1A2
- osteogenesis imperfecta
Data/Specifications
Description: Purified Rat Collagen Type I (Atelocollagen) from rat tail tendon
Form: Lyophilized, salt free, 30 mg/ vial
Sterility: Passed sterility test for bacteria and fungi
Source: Rat tail tendons
Purity: Rat collagen Type I > 90% / Rat collagen type III <10%
Purification: Collagen was extracted from dissected tissue into diluted acetic acid after mild pepsin treatment. Collagen type I was purified by using differential salt precipitation.
Reconstitution: Dissolve in 20 mM acetic acid. Suspension should be shaken or stirred for several hours or overnight between 2-8 °C. Material is dissolved when it appears homogeneous, with no schlieren pattern (light refraction due to differing protein concentrations in the suspensions). Recommended concentration 1-5 mg/mL.
Storage: Collagen dissolved in acetic acid is stable at 4°C for 1 month. Lyophilized collagen long term storage at -20°C or lower.
Applications: Cell culture (coating or 3D gel)
Literature/Support
Purified Rat Collagen Type I (Atelocollagen) from rat tail tendon Insert (PDF)
Note: inserts are for review only. Please use the insert that was sent with the product to ensure that the correct revision insert is being used for the product purchased.
How To Use
Dissolve in 20 mM acetic acid. Suspension should be shaken or stirred for several hours or overnight between 2-8°C. Material is dissolved when it appears homogeneous, with no schlieren pattern (light refraction due to differing protein concentrations in the suspensions). Recommended concentration 1-5 mg/mL.
Related Products
ebiomall.com
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因为DNA和RNA链上的碱基都基本一致。而这两种上样缓冲液中含有的染料是二甲苯青和溴酚蓝，都可以与其碱基结合，跑出条带。
但是，如果使用DNA上样缓冲液，里面会夹杂一些RNA酶，这些酶有可能会影响到实验结果。使用前需要将RNA酶处理掉。
1mol/l Tirs-HCl(PH8.8) 0.6ml
10%SDS 2ml
50%甘油 5ml
2-巯基乙醇 0.5ml
1%溴酚兰 1ml
水 0.9ml
另外：SDS不好溶解建议加热溶解,如果要做非还原SDS-PAGE请将2-巯基乙醇 0.5ml换成超纯水即可
配制分三步： 1) 1 M Tris-HCl (pH 8.0) 50 ml的配制：称取Tris碱6.06 g，加超纯水40 ml溶解，滴加浓HCl约2.1 ml调pH至8.0，定容至50 ml。
