![NanoHelix/qRT-PCR Kit [v4] (UDG System), Bulk/Custom/BCVQR4](images/no_picture.gif)
qRT-PCR Kit [v4] (UDG System), Bulk
- qRT-PCR Kit [v4] reagent suitable for molecular diagnosis of COVID-19
- Enzyme production for qRT-PCR Kit [v4] : Up to 20 million tests per week
- Verify test method according to WHO (17 Jan, 2020), (Using SARS-CoV-2 viral RNA & RdRp/E gene-specific primer/probe)
- With or without UDG system [UDG system : Carryover contamination prevention]
Products
Cat.No. | Product | Feature | Size |
---|---|---|---|
BCVQR4 | qRT-PCR Kit [v4] (UDG System), Bulk | + UDG SYSTEM* | Custom |
- Description
- Data
- Specification
- Documents
qRT-PCR Kit [v4] (UDG System) is a quantitative RT-PCR reagent suitable for molecular diagnosis of SARS-CoV-2 causing the new Coronavirus infectious disease (COVID-19). The kit performance was confirmed using the materials and methods approved by WHO on 17 Jan, 2020. The Enzyme Mix contains in this kit, is an optimized blend of Reverse Transcriptase, chemically-modified hot-start PCR enzyme, RNase inhibitor protein and a thermolabile UDG. These components help ensure sensitivity, accuracy, and specificity of PCR performance for the COVID-19 molecular diagnosis. In particular, carryover contaminated PCR products could be removed by the applied UDG system.
Application
- Quantification of target RNA by real-time RT-PCR
Character
- Use the chemically-modified Hot-Start Taq (Hot-Taq)
- Pre-denaturation time in PCR steps = 15 min
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最好是测浓度,一般不超过30-40ug,上样缓冲液一般4×或者5×,这个没太大区别。
1mol/l Tirs-HCl(PH8.8) 0.6ml
10%SDS 2ml
50%甘油 5ml
2-巯基乙醇 0.5ml
1%溴酚兰 1ml
水 0.9ml
另外:SDS不好溶解建议加热溶解,如果要做非还原SDS-PAGE请将2-巯基乙醇 0.5ml换成超纯水即可
蛋白电泳上样缓冲液包括2%SDS , 0.1%溴酚蓝 10%甘油,SDS是保证样品中的所有蛋白带电荷一致,减少电荷对电泳结果的影响。还原剂是让二硫键处于断开状态,保证蛋白分子的线性.
溴酚蓝作用是指示上样蛋白的跑胶时标记的,甘油是增加上样重量的,以免漂浮,煮沸是使蛋白变性的永久保存
0.5 M tris-HCl (pH 6.8) 20%
丙三醇 20%
20%SDS 20%
0.1%溴酚兰 5%
2-巯基乙醇 10%
双蒸水 25%
不调PH值,PH在配0.5 M tris-HCl 时已调,缓冲液呈蓝紫色。

