
Overview:
Product Name | Cyclooxygenase 1 Antibody | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Description | Rabbit Anti-Human Cyclooxygenase 1 Polyclonal | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Species Reactivity | Human, Mouse, Rat | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Applications | WB, ICC/IF | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Antibody Dilution | WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Host Species | Rabbit | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen Species | Human | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen | Synthetic peptide of Human Cyclooxygenase | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Concentration | 1 mg/ml | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Conjugates |
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
StreptavidinProperties:
Streptavidin Datasheet BiotinProperties:
Biotin Datasheet HRP (Horseradish peroxidase)Properties:
HRP Datasheet AP (Alkaline Phosphatase)Properties:
AP Datasheet
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Properties
Storage Buffer | PBS, 50% glycerol, 0.09% sodium azide |
Storage Temperature | -20ºC |
Shipping Temperature | Blue Ice or 4ºC |
Purification | Peptide Affinity Purified |
Clonality | Polyclonal |
Specificity | Detects ~75 kDa. |
Cite This Product | StressMarq Biosciences Cat# SPC-707, RRID: AB_2705987 |
Certificate of Analysis | A 1:1000 dilution of SPC-707 was sufficient for detection of Cyclooxygenase in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. |
Biological Description
Alternative Names | EC:1.14.99.1 Antibody, PGHS1 Antibody, Prostaglandin G/H synthase 1 Antibody, PGHS-1 Antibody, Prostaglandin H2 synthase 1 Antibody, Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) Antibody, PHS 1 Antibody, EC 1.14.99.1 Antibody, Prostaglandin-endoperoxide synthase 1 Antibody, COX 3 Antibody, PTGS1 Antibody, PGH1_HUMAN Antibody, COX-1 Antibody, PGG/HS Antibody, PTGHS Antibody, Cox3 Antibody, PGH synthase 1 Antibody, Cyclooxygenase-1 Antibody, PHS1 Antibody, Cyclooxygenase 1 Antibody, Cyclooxygenase 3, included Antibody, Partial COX1 proteins, included Antibody, COX 1 Antibody, COX1 Antibody, PCOX1 Antibody |
Research Areas | Cancer, Atherosclerosis, Blood, Cancer Lipid and Lipoprotein Metabolism, Cancer Metabolism, Cardiovascular System, Cell Signaling, Immunology, Innate Immunity, Lipid and lipoprotein Metabolism, Macrophage, Metabolism, Metabolism and Oxidative Stress, Platelets, Redox Metabolism |
Cellular Localization | Endoplasmic reticulum membrane, Microsome Membrane, Peripheral membrane protein |
Accession Number | NP_000953.2 |
Gene ID | 5742 |
Swiss Prot | P23219 |
Product Images

Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of 68.7 kDa Cyclooxygenase 1 protein using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 68.7 kDa.

Western blot analysis of Mouse Kidney cell lysates showing detection of 68.7 kDa Cyclooxygenase 1 protein using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Kidney cell lysates. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 68.7 kDa.

Western blot analysis of Rat Brain cell lysates showing detection of 68.7 kDa Cyclooxygenase 1 protein using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 68.7 kDa.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Microsome Membrane, Endoplasmic Reticulum Membrane, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cyclooxygenase 1 Antibody. (D) Composite.
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ATTO 565 | ||
Overview:
ATTO 565 Datasheet | ![]() | Optical Properties: λex = 563 nm λem = 592 nm εmax = 1.2×105 Φf = 0.9 τfl = 3.4 n Brightness = 10 Laser = 532 nm Filter set = TRITC |
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低离子强度时,迁移率快。但离子强度过低,缓冲液的缓冲容量小,不易维持pH恒定。高离子强度时迁移率慢,因此为了获得更快的反应速度,在缓冲容量允许的范围内,离子强度应该尽可能小。
1. Buffer中离子浓度过大,甘氨酸或者Tris碱有可能疏忽多加了
2.没有加相应浓度的SDS
3.电泳周围温度高,也是一个原因
加样缓冲液的主要作用是使PCR产物与其混合,使DNA沉于加样孔的底部,防止DNA跑出来.
2.配好的EDTA不灭菌行不?放4度冰箱等过完年回来直接拿去配TAE
3.TAE配好后不用灭菌吧

