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StressMarq/Anti-Cytochrome P450 Reductase Antibody/SPC-210D-A488/100-µg

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StressMarq/Anti-Cytochrome P450 Reductase Antibody/SPC-210D-A488/100-µg

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SPC-210D-A488

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Overview:

Product Name Cytochrome P450 Reductase Antibody

Description

Rabbit Anti-Rat Cytochrome P450 Reductase Polyclonal

Species Reactivity Human, Mouse, Rat

Applications WB, IHC, ICC/IF

Antibody Dilution WB (1:1000), ICC/IF (1:60); optimal dilutions for assays should be determined by the user.

Host Species Rabbit

Immunogen Species Rat

Immunogen Rat native full-length Cytochrome P450 Reductase purified from liver tissue

Concentration 1 mg/ml

Conjugates

Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

PerCP

Overview:

Peridinin-Chlorophyll-Protein Complex
Small phycobiliprotein
Isolated from red algae
Large stokes shift (195 nm)
Molecular Weight: 35 kDa

PerCP Datasheet

PerCP Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 482 nm

λ_em = 677 nm

ε_max = 1.96 x 10⁶

Laser = 488 nm

PE/ATTO 594

PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.

Overview:

High fluorescence yield
High photostability
Very hydrophilic
Excellent solubility in water
Very little aggregation

PE/ATTO 594 Datasheet

PE-ATTO 594 Fluorophore Conjugate Excitation and Emission Spectra

Optical Properties:

λ_ex = 535 nm

λ_em = 627 nm

Laser = 488 to 561 nm

FITC (Fluorescein)

Overview:

Excellent fluorescence quantum yield
High rate of photobleaching
Good solubility in water
Broad emission spectrum
pH dependent spectra
Molecular formula: C₂₀H₁₂O₅
Molar mass: 332.3 g/mol

FITC-Fluorescent-conjugate

FITC Fluorescein Fluorophore Excitation and Emission Spectra

Optical Properties:

λ_ex = 494 nm

λ_em = 520 nm

ε_max = 7.3×10⁴

Φ_f = 0.92

τ_fl = 5.0 ns

Brightness = 67.2

Laser = 488 nm

Filter set = FITC

ATTO 700

Overview:

High fluorescence yield
Excellent thermal and photostability
Quenched by electron donors
Very hydrophilic
Good solubility in polar solvents
Zwitterionic dye
Molar Mass: 575 g/mol

ATTO 700 Datasheet

ATTO 700 Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 700 nm

λ_em = 719 nm

ε_max = 1.25×10⁵

Φ_f = 0.25

τ_fl = 1.6 ns

Brightness = 31.3

Laser = 676 nm

Filter set = Cy®5.5

ATTO 680

Overview:

High fluorescence yield
Excellent thermal and photostability
Quenched by electron donors
Very hydrophilic
Good solubility in polar solvents
Zwitterionic dye
Molar Mass: 631 g/mol

ATTO 680 Datasheet

ATTO 680 Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 680 nm

λ_em = 700 nm

ε_max = 1.25×10⁵

Φ_f = 0.30

τ_fl = 1.7 ns

Brightness = 37.5

Laser = 633 – 676 nm

Filter set = Cy®5.5

ATTO 655

Overview:

High fluorescence yield
High thermal and photostability
Excellent ozone resistance
Quenched by electron donors
Very hydrophilic
Good solubility in polar solvents
Zwitterionic dye
Molar Mass: 634 g/mol

ATTO 655 Datasheet

ATTO 655 Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 663 nm

λ_em = 684 nm

ε_max = 1.25×10⁵

Φ_f = 0.30

τ_fl = 1.8 ns

Brightness = 37.5

Laser = 633 – 647 nm

Filter set = Cy®5

ATTO 633

Overview:

High fluorescence yield
High thermal and photostability
Moderately hydrophilic
Good solubility in polar solvents
Stable at pH 4 – 11
Cationic dye, perchlorate salt
Molar Mass: 652.2 g/mol

ATTO 633 Datasheet

ATTO 633 Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 629 nm

λ_em = 657 nm

ε_max = 1.3×10⁵

Φ_f = 0.64

τ_fl = 3.2 ns

Brightness = 83.2

Laser = 633 nm

Filter set = Cy®5

ATTO 594

Overview:

High fluorescence yield
High photostability
Very hydrophilic
Excellent solubility in water
Very little aggregation
New dye with net charge of -1
Molar Mass: 1137 g/mol

ATTO 594 Datasheet

ATTO 594 Fluorophore Excitation and Emission Spectrum

Optical Properties:

λ_ex = 601 nm

λ_em = 627 nm

ε_max = 1.2×10⁵

Φ_f = 0.85

τ_fl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

ATTO 565

Overview:

High fluorescence yield
High thermal and photostability
Good solubility in polar solvents
Excellent solubility in water
Very little aggregation
Rhodamine dye derivative
Molar Mass: 611 g/mol

ATTO 565 Datasheet

ATTO 565 Fluorophore Excitation and Emission Spectra

Optical Properties:

λ_ex = 563 nm

λ_em = 592 nm

ε_max = 1.2×10⁵

Φ_f = 0.9

τ_fl = 3.4 n

Brightness = 10

Laser = 532 nm

Filter set = TRITC

ATTO 488

Overview:

High fluorescence yield
High photostability
Very hydrophilic
Excellent solubility in water
Very little aggregation
New dye with net charge of -1
Molar Mass: 804 g/mol

ATTO 488 Datasheet

ATTO 488 Fluorophore Excitation and Emission Spectra

Optical Properties:

λ_ex = 501 nm

λ_em = 523 nm

ε_max = 9.0×10⁴

Φ_f = 0.80

τ_fl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

ATTO 390

Overview:

High fluorescence yield
Large Stokes-shift (89 nm)
Good photostability
Moderately hydrophilic
Good solubility in polar solvents
Coumarin derivate, uncharged
Low molar mass: 343.42 g/mol

ATTO 390 Datasheet

ATTO 390 Fluorescent Dye Excitation and Emission Spectra

Optical Properties:

λ_ex = 390 nm

λ_em = 479 nm

ε_max = 2.4×10⁴

Φ_f = 0.90

τ_fl = 5.0 ns

Brightness = 21.6

Laser = 365 or 405 nm

APC (Allophycocyanin)

Overview:

High quantum yield
Large phycobiliprotein
6 chromophores per molecule
Isolated from red algae
Molecular Weight: 105 kDa

APC Datasheet

APC Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 650 nm

λ_em = 660 nm

ε_max = 7.0×10⁵

Φ_f = 0.68

Brightness = 476

Laser = 594 or 633 nm

Filter set = Cy®5

Streptavidin

Properties:

Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
Molecular weight: 53 kDa
Formula: C₁₀H₁₆N₂O₃S
Applications: Western blot, immunohistochemistry, and ELISA

Streptavidin Datasheet

Biotin

Properties:

Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity
Molar mass: 244.31 g/mol
Formula: C₁₀H₁₆N₂O₃S
Applications: Western blot, immunohistochemistry, and ELISA

Biotin Datasheet

HRP (Horseradish peroxidase)

Properties:

Enzymatic activity is used to amplify weak signals and increase visibility of a target
Readily combines with hydrogen peroxide (H₂O₂) to form HRP-H₂O₂ complex which can oxidize various hydrogen donors
Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
High turnover rate enables rapid generation of a strong signal
44 kDa glycoprotein
Extinction coefficient: 100 (403 nm)
Applications: Western blot, immunohistochemistry, and ELISA

HRP Datasheet

AP (Alkaline Phosphatase)

Properties:

Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
Molecular weight: 140 kDa
Applications: Western blot, immunohistochemistry, and ELISA

AP Datasheet

R-PE (R-Phycoerythrin)

Overview:

Broad excitation spectrum
High quantum yield
Photostable
Member of the phycobiliprotein family
Isolated from red algae
Excellent solubility in water
Molecular Weight: 250 kDa

R-PE Datasheet

R-PE Fluorophore Excitation and Emission Spectra

Optical Properties:

λ_ex = 565 nm

λ_em = 575 nm

ε_max = 2.0×10⁶

Φ_f = 0.84

Brightness = 1.68 x 10³

Laser = 488 to 561 nm

Filter set = TRITC

Properties

Storage Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Storage Temperature	-20ºC
Shipping Temperature	Blue Ice or 4ºC
Purification	Protein A purified
Clonality	Polyclonal
Specificity	Detects ~77kDa.
Cite This Product	StressMarq Biosciences Cat# SPC-210, RRID: AB_2704289
Certificate of Analysis	1 µg/ml of SPC-210 was sufficient for detection of Cytochrome P450 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names	Cytochrome C Reductase Antibody, CCR Antibody, CPR Antibody, P450R Antibody, CYPOR Antibody, DKFZp686G04235 Antibody, FLJ26468 Antibody, NADPH Cytochrome Antibody, P450 Reductase Antibody, NADPH dependent cytochrome P450 reductase Antibody, NADPH-cytochrome P450 reductase Antibody, P450 (cytochrome) oxidoreductase Antibody, P450 Cytochrome Oxidoreductase Antibody, P450R Antibody, POR Antibody
Research Areas	Cancer, Heat Shock, Cardiovascular System
Cellular Localization	Endoplasmic Reticulum, Endoplasmic reticulum membrane, Microsome
Accession Number	NP_113764.1
Gene ID	29441
Swiss Prot	P00388
Scientific Background	Cytochrome P450 Reductase is an enyme that is required for the transfer of electrons from NADPH to cytochrome P450 in microsomes. It has also been found to transfer electrons to Heme oxygenase and cytochrome B5. Cytochrome P450 Reductase is found localized to the endoplasmic reticulum membrane, tethered by its hydrophobic N-terminal tail. Mutations in the gene encoding Cytochrome P450 Reductase (POR) have been found to cause congenital adrenal hyperplasia, Antley-Bixler syndrome, amenorrhea and disordered steroidogenesis.
References	1. Pandey A.V. and Flück C.E. (2013). Pharmacology & therapeutics 138 (2): 229–54. 2. Jensen K. and Møller BL (2010). Phytochemistry 71 (2-3): 132–41. 3. Flück C.E. et al. (2004). Nature Genetics 36 (3): 228–230.

Product Images

<p>Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cytochrome P450 Reductase Polyclonal Antibody (SPC-210). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Cytochrome P450 Reductase Polyclonal Antibody (SPC-210) at 1:60 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Cytosol. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Cytochrome P450 Reductase Antibody. (C) Composite. Heat Shocked at 42°C for 1h.</p>

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cytochrome P450 Reductase Polyclonal Antibody (SPC-210). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Cytochrome P450 Reductase Polyclonal Antibody (SPC-210) at 1:60 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Cytosol. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Cytochrome P450 Reductase Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

<p>Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cytochrome P450 Reductase Polyclonal Antibody (SPC-210). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Cytochrome P450 Reductase Polyclonal Antibody (SPC-210) at 1:150 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Cytosol. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Cytochrome P450 Reductase Antibody. (C) Composite.</p>

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cytochrome P450 Reductase Polyclonal Antibody (SPC-210). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Cytochrome P450 Reductase Polyclonal Antibody (SPC-210) at 1:150 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Cytosol. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Cytochrome P450 Reductase Antibody. (C) Composite.

<p>Western blot analysis of Human, Mouse, Rat Human, Mouse and Rat Lysates showing detection of ~ 75 kDa Cytochrome P450 Reductase protein using Rabbit Anti-Cytochrome P450 Reductase Polyclonal Antibody (SPC-210). Lane 1: MW ladder. Lane 2: Human lysate, A431. Lane 3: Mouse lung lysate. Lane 4: Rat lysate, Rat Brain Membrane (RBM). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Cytochrome P450 Reductase Polyclonal Antibody (SPC-210) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP antibody at 1:2000 for 1 hour at RT. Color Development: TMB solution for 11 min at RT. Predicted/Observed Size: ~ 75 kDa. Other Band(s): ~30 -250 in Rat lysate only.</p>

Western blot analysis of Human, Mouse, Rat Human, Mouse and Rat Lysates showing detection of ~ 75 kDa Cytochrome P450 Reductase protein using Rabbit Anti-Cytochrome P450 Reductase Polyclonal Antibody (SPC-210). Lane 1: MW ladder. Lane 2: Human lysate, A431. Lane 3: Mouse lung lysate. Lane 4: Rat lysate, Rat Brain Membrane (RBM). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Cytochrome P450 Reductase Polyclonal Antibody (SPC-210) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP antibody at 1:2000 for 1 hour at RT. Color Development: TMB solution for 11 min at RT. Predicted/Observed Size: ~ 75 kDa. Other Band(s): ~30 -250 in Rat lysate only.

Product Citations (1)

Western Blot

Effect of Genistein on the Expression of Testicular Steroidogenic and Drug-Metabolizing Enzymes in Adult Male Sprague-Dawley Rats.

Chung, H. (2017) University of British Columbia. Dissertation.

PubMed ID: N/A Reactivity Rat Applications: Western Blot

ATTO 488

Overview:

High fluorescence yield
High photostability
Very hydrophilic
Excellent solubility in water
Very little aggregation
New dye with net charge of -1
Molar Mass: 804 g/mol

ATTO 488 Datasheet

Optical Properties:

λ_ex = 501 nm

λ_em = 523 nm

ε_max = 9.0×10⁴

Φ_f = 0.80

τ_fl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

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乙酸龙脑酯是由河南莱尔茵生物科技有限公司代理或销售的莱尔茵品牌的试剂，产品来源于河南洛阳。河南莱尔茵生物科技有限公司是中国最权威的乙酸龙脑酯试剂销售服务商之一，在洛阳等地方销售乙酸龙脑酯试剂已经多年。生物在线为您提供众多企业乙酸龙脑酯仪器产品及图片，以便挑选到性价比高，合适的乙酸龙脑酯产品查看更多>

DPD试剂盒,二氢嘧啶脱氢酶试剂盒快速检测 [DPD试剂盒,二氢嘧啶...

2019-03-07

琼脂糖凝胶电泳缓冲液配制有哪几点琼脂糖凝胶具有网络结构，物质分子通过时会受到阻力，大分子物质在涌动时受到的阻力大，因此在凝胶电泳中，带电颗粒的分离不仅取决于净电荷的性质查看更多>

足迹法学术百科知网空间

2018-12-19

KRPH缓冲液（500ml）无菌溶液现配现用！中文名称：KRPH缓冲液规格：500ml用途：Sigma-MAK083 KREBS-RIN 查看更多>

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商品咨询

SDSPAGE上样缓冲液(非还原,5×)性能参数,报价/价格,图片中国...123

2021-08-04

保存得当，确保缓冲液没受污染，没长毛的话，就可以用。
但是最好还是不要用，影响实验就不好了。

电泳法分离蛋白质时,缓冲液的离子强度的一般要求是A.0.01～0.05B....123

想自由zJ72021-07-28

离子强度是表示溶液中电荷数量的一种量度，等于溶液中各种离子的摩尔浓度与其价数平方之积总和的一半。
低离子强度时，迁移率快。但离子强度过低，缓冲液的缓冲容量小，不易维持pH恒定。高离子强度时迁移率慢，因此为了获得更快的反应速度，在缓冲容量允许的范围内，离子强度应该尽可能小。

RNA电泳为什么要用MOPS缓冲液123

嗳情啖了﹎4142021-08-08

电泳缓冲液还有一个组分是EDTA（乙二胺四乙酸），加入浓度为1-2mmol/L，目的是螯合Mg2+ 等离子，防止电泳时激活 DNA酶，此外还可防止Mg2+离子与核酸生成沉淀。
此外，我们常常用“电泳法”判断液体的性质，是胶体还是溶液。在高中化学中我们就用过这种方法判断给定的液体是否为胶体。MOPS电泳缓冲液、胚胎干细胞培养生长因子、动物肝细胞分离培养试剂盒、结晶紫细胞群落染色试剂盒、MFN-2蛋白表达检测试剂盒、血红细胞溶解液、磷酸缓冲盐溶液、Hanks平衡盐粉剂、胰蛋白酶溶液、

Yeast Nuclei Isolation酵母生物在线 LabonWeb123

chromosomedx2021-07-26

在《分子克隆》上看见检测微量的DNA可以使用先电泳，再用含EB的电泳缓冲液染色30至45分钟。
我使用的是1%琼脂糖凝胶，电泳缓冲液为0.5×TBE。
请问各位大虾，在不影响实验结果的前提下，这样的含EB的电泳缓冲液可以反复使用多少次？

谢谢^_^

第二向sdspage,电泳缓冲液可以多次使用吗? 生物科学 ...123

chromosomedx2007-08-12

在《分子克隆》上看见检测微量的DNA可以使用先电泳，再用含EB的电泳缓冲液染色30至45分钟。
我使用的是1%琼脂糖凝胶，电泳缓冲液为0.5×TBE。
请问各位大虾，在不影响实验结果的前提下，这样的含EB的电泳缓冲液可以反复使用多少次？

谢谢^_^

有学预防的前辈吗?电泳后,正、负极槽内电泳缓冲液pH会怎样变化? ...123

曌猴速矩512021-08-05

将会降低

【求助/交流】蛋白电泳缓冲液分子生物综合其他论坛...123

coolaber2021-07-20

北京COOLABER隆重推出PBS、TAE、TBE、蛋白电泳缓冲液独立包装预混干粉试剂，配缓冲液像冲咖啡一样简单，您只需要准备容器和水，就一切OK啦！什么计算配比，什么称重，什么调PH值，都去他的！联系电话：010-8244451715132878855400-878-6800QQ:1002073414李云云

【求助】为什么我的SDSPAGE电泳时电泳缓冲液变浑浊呢?123

戀█重量█p82017-10-02

原因可能如下：
1. Buffer中离子浓度过大，甘氨酸或者Tris碱有可能疏忽多加了
2.没有加相应浓度的SDS
3.电泳周围温度高，也是一个原因

【求助】人血清脂蛋白琼脂糖凝胶电泳缓冲液蛋白质和糖学技术...123

czp862021-07-22

本人对电泳了解不多，现在有个问题向大家求教；我做的是人血清脂蛋白琼脂糖凝胶电泳，我看文献上基本上是写的是用Tris-巴比妥缓冲液，但现在巴比妥和巴比妥钠很贵，用量很大，而且还买不到！我想问下可不可用其他什么缓冲液代替一下？请帮忙，谢谢大家了

SDSPAGE电泳相关溶液的配置123

qinhuan04252021-08-01

最近刚开始跑SDS-PAGE，很奇怪，加样后，Marker和样本停在加样孔不往下面跑！且跑电泳时，电压（80V）一直上不去，后测电泳液PH值，不到8.0，这是主要原因吗？

如需调PH，是用NaOH吗？

高效毛细管电泳思考题和答案解析123

aileenss2008-03-26

1.高效毛细管电泳中的缓冲液两瓶里装的是一种吗？
2.如果不是，两种缓冲液怎么选择？哪个放在阳极，哪个放在阴极？哪边是阳极？哪边是阴极？进样品的一端是阴极吗？
3.缓冲液的pH怎么确定？
让各位见笑了！谢谢！