
Overview:
Product Name | UVRAG Antibody | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Description | Rabbit Anti-Human UVRAG Polyclonal | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Species Reactivity | Human, Mouse, Rat | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Applications | WB, ICC/IF | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Antibody Dilution | WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Host Species | Rabbit | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen Species | Human | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Immunogen | Synthetic peptide from the C-terminal of human UVRAG | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Concentration | 1 mg/ml | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Conjugates |
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
StreptavidinProperties:
Streptavidin Datasheet BiotinProperties:
Biotin Datasheet HRP (Horseradish peroxidase)Properties:
HRP Datasheet AP (Alkaline Phosphatase)Properties:
AP Datasheet
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Properties
Storage Buffer | PBS, 50% glycerol, 0.09% sodium azide |
Storage Temperature | -20ºC |
Shipping Temperature | Blue Ice or 4ºC |
Purification | Peptide Affinity Purified |
Clonality | Polyclonal |
Specificity | Predicted molecular weight at ~78.1kDa. Observed molecular weights in the 75-90kDa range. |
Cite This Product | StressMarq Biosciences Cat# SPC-605, RRID: AB_2704799 |
Certificate of Analysis | A 1:1000 dilution of SPC-605 was sufficient for detection of UVRAG on 293T Rapamycin treated lysates using Goat anti-rabbit IgG:HRP as the secondary antibody. |
Biological Description
Alternative Names | DHTX Antibody, p63 Antibody, UV radiation resistance associated Antibody, UVRAG_HUMAN Antibody |
Research Areas | Cancer, Apoptosis, Autophagy, Cardiovascular System, Cell Signaling, Epigenetics and Nuclear Signaling, Heart |
Cellular Localization | centromere, Chromosome, Endoplasmic Reticulum, Endosome, Lysosome, Nucleus |
Accession Number | NP_003360.2 |
Gene ID | 7405 |
Swiss Prot | Q9P2Y5 |
Scientific Background | UVRAG (UV radiation resistance-associated gene) is associated with the Beclin-1/PI3KC3 complex and promotes PI3KC3 enzymatic activity and autophagy, while suppressing proliferation (1). Beclin-1 binding to UVRAG promotes both autophagosome maturation and endocytic trafficking (2). UVRAG is also a potential tumor suppressor protein with frameshift mutations observed in colon and gastric carcinomas (3-4). It is highly expressed in the brain, lung, kidney and liver. |
References |
1. Liang, C. et al. (2008) Nat Cell Biol. 10: 776-87. 2. Ionov, Y. et al. (2004) Oncogene. 23: 639-45. 3. Kim, M.S. et al. (2008) Hum Pathol. 39: 1059-63. |
Product Images

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-UVRAG Polyclonal Antibody (SPC-605). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-UVRAG Polyclonal Antibody (SPC-605) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Late Endosome, Lysosome, Early Endosome. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) UVRAG Antibody (D) Composite.

Western blot analysis of Human 293T showing detection of ~70kDa UVRAG protein using Rabbit Anti-UVRAG Polyclonal Antibody (SPC-605). Lane 1: MW Ladder. Lane 2: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-UVRAG Polyclonal Antibody (SPC-605) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~70kDa.

Western blot analysis of Rat Liver showing detection of ~70kDa UVRAG protein using Rabbit Anti-UVRAG Polyclonal Antibody (SPC-605). Lane 1: MW Ladder. Lane 2: Rat Liver (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-UVRAG Polyclonal Antibody (SPC-605) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~70kDa.
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Biotin
Properties:
- Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity
- Molar mass: 244.31 g/mol
- Formula: C10H16N2O3S
- Applications: Western blot, immunohistochemistry, and ELISA
Biotin Datasheet
ebiomall.com






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TAE是使用最广泛的缓冲系统。其特点是超螺旋在其中电泳时更符合实际相对分子质量(TBE中电泳时测出的相对分子质量会大于实际分子质量),且双链线状DNA在其中的迁移率较其他两种缓冲液快约10%,电泳大于13kb的片段时用TAE缓冲液将取得更好的分离效果,此外,回收DNA片段时也易用TAE缓冲系统进行电泳。TAE的缺点是缓冲容量小,长时间电泳(如过夜)不可选用,除非有循环装置使两极的缓冲液得到交换。 50×TAE Buffer 配制方法: 1. 称量Tris 242g,Na2EDTA.2H2O 37.2g 于1L烧杯中; 2.向烧杯中加入约800ml去离子水,充分搅拌均匀; 3加入57.1ml的冰乙酸,充分溶解; 4.加去离子水定容至1L后,室温保存。向左转|向右转
1. Buffer中离子浓度过大,甘氨酸或者Tris碱有可能疏忽多加了
2.没有加相应浓度的SDS
3.电泳周围温度高,也是一个原因
此外,我们常常用“电泳法”判断液体的性质,是胶体还是溶液。在高中化学中我们就用过这种方法判断给定的液体是否为胶体。MOPS电泳缓冲液、胚胎干细胞培养生长因子、动物肝细胞分离培养试剂盒、结晶紫细胞群落染色试剂盒、MFN-2蛋白表达检测试剂盒、血红细胞溶解液、磷酸缓冲盐溶液、Hanks平衡盐粉剂、胰蛋白酶溶液、
低离子强度时,迁移率快。但离子强度过低,缓冲液的缓冲容量小,不易维持pH恒定。高离子强度时迁移率慢,因此为了获得更快的反应速度,在缓冲容量允许的范围内,离子强度应该尽可能小。

