请使用支持JavaScript的浏览器! 正品Tocris生物试剂1 Tocris Bioscience一级代理,Tocris/Janelia Fluor<sup>®</sup> 549, SE/6147/5/5 mg,荧光染料,染色剂,生化试剂,,Tocris Bioscience蚂蚁淘商城
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Tocris/Janelia Fluor<sup>®</sup> 549, SE/6147/5/5 mg
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Tocris/Janelia Fluor® 549, SE/6147/5/5 mg
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Tocris
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6147/5
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Description:Cellpermeablefluorescentdye;suppliedasNHSester
AlternativeNames:JF549,NHS
ChemicalName:3,6-Di-1-azetidinyl-9-[2-carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]xanthylium,innersalt
BIOLOGicalActivityTechnicalDataSolubilityCalculatorsDatasheetsReferences

BiologicalActivity

Fluorescentdye;suppliedasanNHSesterforcouplingtoprimaryaminegroups.CompatIBLewithself-labelingtagsystems,e.g.HaloTag®andSNAP-tag®.Suitableforconfocalfluorescentimagingandsuperresolutionmicroscopy(SRM)techniques,suchasdSTORM(liveandfixedcells).Cellpermeable.Excitationmaximum=549nm;emissionmaximum=571nm;Quantumyield=0.88;Extinctioncoefficient=101,000M-1cm-1;A280correctionfactoris0.169.JaneliaFluor®646SE(Cat.No.6148)alsoavailable.

HaloTagisatrademarkofPromegaCorporation,andSNAP-tagisatrademarkofNewEnglandBiolabs,Inc.

LicensingInformation

SoldunderlicensefromtheHowardHughesMedicalInstitute,JaneliaResearchCampus

ApplicationofJaneliaFluor®DyesinCardiacTissue

ApplicationofJaneliaFluor®DyesinCardiacTissue-Leftimage-Widefieldfluorescenceimageofmuscletissuefromanadultpigheart,displayingthedistributionofcollagenVIintheinterstitialspacebetweenheartmusclecells.ThissamplewaslabelledwithaprimaryantibodyagainstcollagenVIandasecondaryantibodyconjugatedtoJF549(Cat.No.6147).Scalebar:50μm.Middleimage-Anadultpighearttissuesection,10μmthick,labelledwithantibodiesagainstSERCA2ATPase,exhibitstheintricatestructureofthesarcoplasmicreticulum.Asuper-resolutionimage(toppanel),obtainedbyexploitingspontaneousphoto-switchingofJF549(dSTORM),showsfarsuperiordetailthanthediffractionlimitedwidefieldimage(bottom).Scalebar:200nm.Rightimage-AsingleisolatedWistarratcardiomyocytestainedagainstα-actinin,displayingitsperiodicstructurelocalisedattheendsofsarcomeres(Z-discs).WidefieldfluorescenceofJF549(leftpanel),priortoilluminatingwitha561nmlasertoinducephotoswitchingandproduceadSTORMsuper-resolutionimage(right).Scalebar:1μm.AllimageskindlyprovidedbyProf.ChristianSoeller,UniversityofExeter,acquiredbyAlexClowsleyandAnnaMeletiou.">Application of Janelia Fluor® Dyes in Cardiac Tissue

ApplicationofJaneliaFluor®DyesinCardiacTissue-Leftimage-Widefieldfluorescenceimageofmuscletissuefromanadultpigheart,displayingthedistributionofcollagenVIintheinterstitialspacebetweenheartmusclecells.ThissamplewaslabelledwithaprimaryantibodyagainstcollagenVIandasecondaryantibodyconjugatedtoJF549(Cat.No.6147).Scalebar:50μm.Middleimage-Anadultpighearttissuesection,10μmthick,labelledwithantibodiesagainstSERCA2ATPase,exhibitstheintricatestructureofthesarcoplasmicreticulum.Asuper-resolutionimage(toppanel),obtainedbyexploitingspontaneousphoto-switchingofJF549(dSTORM),showsfarsuperiordetailthanthediffractionlimitedwidefieldimage(bottom).Scalebar:200nm.Rightimage-AsingleisolatedWistarratcardiomyocytestainedagainstα-actinin,displayingitsperiodicstructurelocalisedattheendsofsarcomeres(Z-discs).WidefieldfluorescenceofJF549(leftpanel),priortoIlluminatingwitha561nmlasertoinducephotoswitchingandproduceadSTORMsuper-resolutionimage(right).Scalebar:1μm.

AllimageskindlyprovidedbyProf.ChristianSoeller,UniversityofExeter;acquiredbyAlexClowsleyandAnnaMeletiou.

TechnicalData

M.Wt551.56
FormulaC31H25N3O7
StorageStoreat-20°C
CASNumber1811539-32-8
PubChemID124201856
InChIKeyDWUIXAGWHCMXHN-UHFFFAOYSA-N
SmilesO=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CCC4)C=C3O5)=C(C=C/6)C5=CC6=[N+]7CCC7)=C2

Thetechnicaldataprovidedaboveisforguidanceonly.ForbatchspecificdatarefertotheCertificateofAnalysis.

AllTocrisproductsareintendedforlaboratoryresearchuseonly.

SolubilityData

SolventMaxConc.mg/mLMaxConc.mM
Solubility
DMSO55.16100

PreparingStockSolutions

Thefollowingdataisbasedontheproductmolecularweight551.56.Batchspecificmolecularweightsmayvaryfrombatchtobatchduetosolventofhydration,whichwillaffectthesolventvolumesrequiredtopreparestocksolutions.

Concentration/SolventVolume/Mass1mg5mg10mg
1mM1.81mL9.07mL18.13mL
5mM0.36mL1.81mL3.63mL
10mM0.18mL0.91mL1.81mL
50mM0.04mL0.18mL0.36mL

ProductDatasheets

CertificateofAnalysis/ProductDatasheet
SafetyDatasheet