The 3G3 antibody reacts with mouse Foxp3, a 50-55 kDa transcription factor which is a central regulator of T cell activity and is critical for the development and function of regulatory T cells (Tregs). Foxp3 is expressed at constitutively high levels in Treg cells, which are further identified as being CD4+ CD25+. In resting conventional T cells (CD4+ CD25-) Foxp3 expression is restricted, and upon TCR activation is expressed only transiently and in a small proportion of cells. However, the growth factor TGF-beta has been shown to induce expression of Foxp3 in na-ve T cells, driving their development into Foxp3+ Tregs, which are called -induced- or -adaptive- Tregs. These cells are phenotypically similar to so-called -natural- Tregs (CD4+ CD25high Foxp3+) which originate in the thymus and comprise the majority of Treg cells. Tregs are critical for maintaining peripheral tolerance and are implicated in the development of autoimmunity.
It is important to review the literature in choosing an antibody for the Foxp3 antigen in flow cytometry, as the potential for high background or non-specific staining may be observed. The 3G3 antibody may be used for intracellular detection of Foxp3 in cells from mouse and Rhesus macaque.
Ramos RN, Oliveira CE, Gasparoto TH, et al. 2012. Carcinogenesis. 33: 902-909. (Flow cytometry)
Klein M, Vaeth M, Scheel T, Grabbe S, Baumgrass R, Berberich-Siebelt F, Bopp T, Schmitt E, and Becker C. 2012. J. Immunol. 188: 1091-1097. (Flow cytometry)
Ansari AA, Reimann KA, Mayne AE, Takahashi Y, Stephenson ST, Wang R, Wang X, Li J, Price AA, Little DM, Zaidi M, Lyles R, and Villinger F. 2011. J. Immunol. 186: 1044-1059. (Flow cytometry - Rhesus macaque)
Nagar M, Vernitsky H, Cohen Y, Dominissini D, Berkun Y, Rechavi G, Amariglio N, and Goldstein I. 2008. Int. Immunol. 20: 1041-1055. (Flow cytometry)
Hombach AA, Kofler D, Hombach A, Rappl G, and Abken H. 2007. J. Immunol. 179: 7924-7931. (Flow cytometry).
Gavin MA, Torgerson TR, Houston E, deRoos P, Ho WY, Stray-Pedersen A, Ocheltree EL, Greenberg PD, Ochs HD, and Rudensky AY. 2006. Proc Natl Acad Sci USA. 103(17): 6659-6664. (Flow cytometry)
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而且样品中的无水硫酸钠未变色,而做标准曲线的五个和空白对照的变为蓝色了,请高手指教,多谢!
还有,是否变蓝对测定结果有影响吗?
谢了哈
欢迎你！请下次规范发贴:)
健那绿——高中唯一一个活体染色剂。染线粒体的，染成蓝绿色
健那绿染液是一种活体染液，实验对象必须是活细胞，健那绿可以使活细胞中的线粒体呈现蓝绿色，而细胞质接近无色。
请各位大侠给予帮助！！
谢谢！！
产品主要应用：点击化学(Clickchemistry)、蛋白质组学研究中的双向荧光差异凝胶电泳(2DDIGE)和实时荧光定量PCR(RealtimePCR)。
氨基类染料是包含自由氨基的活性染料，染料可与活化羧酸衍生物和其他亲电子的试剂结合。比如：氨基与EDC-活化的羧基结合。
相关产品如下：
中文名英文名产品编号分子结构Cy7.5胺Cy7.5amineAGF1350A[img]/KindEditor_4.0.1/attached/image/20130704/20130704110804_5250.jpg[/img]Cy5胺Cy5amineAGF1332A[img]/KindEditor_4.0.1/attached/image/20130704/20130704110715_7750.jpg[/img]相似系列产品：
抗体、核酸、蛋白质等生物分子标记染料
羰基活性荧光染料
巯基反应性染料
羧酸类染料
1、快讯上是Invotrogen公司的SYRBGOLD，不过500ul/1390元，实在有点贵，为了查用量；
2、结果又查出赛百盛的GoldViewTMDNA染料1ml/100元；
3、还有一种在日光下即可看出DNA条带的上海华舜生物工程有限公司的LightBluedye；
为了一个EB，居然不小心查出三种染料，而对这三种染料的评价也褒贬不一，我个人希望找个毒害小些，而且灵敏度还不能低于EB，价格方面也能让老板接受的来。
而对这三个染料，我有三个问题：
1、SYRBGOLD的用量问题，500ul能用到200次吗（我一般一次用30ml的胶，配在胶中）？
2、GoldViewTMDNA染料的成分是否如有些人所说的就是丫啶橙（能举出确切证据吗）？
3、LightBluedye灵敏度如何？能比得过EB吗？
2、苏丹三 脂肪 橙红
3、苏丹四 脂肪 红
4、双缩脲 蛋白质 紫
5、龙胆紫 染色质 紫
6、碘 淀粉 蓝
7、健那绿 线粒体 绿
8、甲基绿 DNA 绿
9、吡罗红 RNA 红
10、溴麝香草酚蓝 CO2 由蓝变绿再变黄
11、重铬酸钾 酒精 酸性条件下由橙色变成灰绿
12、醋酸洋红（龙胆紫、改良苯酚品红） 染色质 红
13、台盼蓝 检验活死细胞 死细胞会被染成蓝色（不常用）
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