
This kit is designed for extraction of nucleic acid (DNA/RNA) in human biological fluids such as nasopharyngeal swabs, sputum, broncho lavage fluid and alveolar lavage fluid. This kit provides all necessary reagents for highly pure viral nucleic acid (DNA/RNA) extraction from samples such as human serum, plasma, urine, cell culture supernatant, nasopharyngeal swabs, sputum, ascites and broncho lavage fluid. The extracted pure viral nucleic acid can be directly applied for Reverse Transcription, PCR, RT-qPCR, RT-PCR, Next Generation Sequencing, Northern Blot and other related assays. Based on the technology of purification of silica gel column, samples are lysed in the high concentration guanidine salt lysis solution, so the nucleic acid could be absorbed on filter membrane by the interaction of hydrogen bond and electrostatic, while proteins and other impurities cannot be absorbed. This kit enables a simple, fast, safe and efficient operation process to extract viral nucleic acid with a high yield, high purity, stable and reliable quality.
Supplier: | Elabscience |
Supplier-Nr: | EL-XG-04 |
Properties
Application: | Viral nucleic acid (DNA/RNA) extraction |
Database Information
Handling & Safety
Storage: | +4°C |
Shipping: | +4°C (International: °C) |
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如常用于缓冲的Tris、HEPES、MPOS等等,去垢剂如NP40,Tween-20,TritonX100,CHAPS,脱氧胆酸钠,SDS等等,
大家能说说这些试剂的使用区别吗(最好讲讲某些情况下使用一些会优于另一些的例子)
有时候某些裂解液含10%左右甘油,又是为何?
平时我们用习惯了一些配方,可能有时候不太清楚为何有些是可替代的,有些是不可替代的,借此机会想听听大家的见解?
去垢剂作为添加剂生长蛋白质晶体是一项很有用的技术。非离子和两性离子结晶剂在膜蛋白结晶中已经得到很好应用,目前已经变成常规的实验手段。去垢剂在一些可溶蛋白中也起到了一定的作用,提高了晶体的质量和结果的可重复性 ,促进了单晶的生长, 但具体的影响机制尚不明确。其可能原因是去垢剂的引入使结晶液滴中的蛋白分子更具有亲水性,使溶液中同质蛋白质分子增加。向左转|向右转
如要膜蛋白保持原来的结构应采用:非离子型去垢剂.

