ELISA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1)
CCL3-L1; LD78; SCYA3L; SCYA3L1; MIP1AP; MIP1-Alpha-P; Small Inducible Cytokine A3-Like 1; Macrophage Inflammatory Protein 1-Alpha-P; Tonsillar Lymphocyte LD78 Beta
- Product No.SEA026Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.156-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.055ng/mL.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 441 For more details, please contact local distributors!US$ 630 For more details, please contact local distributors!US$ 2835 For more details, please contact local distributors!US$ 5355 For more details, please contact local distributors!US$ 44100 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- Typical Standard Curve
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the ELISA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1)
This assay has high sensitivity and excellent specificity for detection of Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1).No significant cross-reactivity or interference between Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) and analogues was observed.
Recovery of the ELISA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1)
Matrices listed below were spiked with certain level of recombinant Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) and the recovery rates were calculated by comparing the measured value to the expected amount of Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 79-91 | 81 |
| EDTA plasma(n=5) | 85-94 | 89 |
| heparin plasma(n=5) | 93-102 | 97 |
Precision of the ELISA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the ELISA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-102% | 78-101% | 98-105% | 95-105% |
| EDTA plasma(n=5) | 92-99% | 86-101% | 85-103% | 89-98% |
| heparin plasma(n=5) | 85-94% | 83-93% | 95-102% | 87-102% |
Stability of the ELISA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the ELISA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1)
1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 3 times;5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle of the ELISA Kit for Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1)
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| APA026Hu01 | Active Chemokine C-C-Motif Ligand 3 Like Protein 1 (CCL3L1) | Cell culture; Activity Assays. |
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如要膜蛋白保持原来的结构应采用：非离子型去垢剂.
去垢剂作为添加剂生长蛋白质晶体是一项很有用的技术。非离子和两性离子结晶剂在膜蛋白结晶中已经得到很好应用,目前已经变成常规的实验手段。去垢剂在一些可溶蛋白中也起到了一定的作用,提高了晶体的质量和结果的可重复性 ,促进了单晶的生长, 但具体的影响机制尚不明确。其可能原因是去垢剂的引入使结晶液滴中的蛋白分子更具有亲水性,使溶液中同质蛋白质分子增加。向左转|向右转
表面活性剂：通常由一个极性头和一个非极性尾组成，用于去除难溶于水的污渍
漂白剂：通常含次氯酸盐成分，原理为其氧化性
稀盐酸：用于去除碱性的污渍，如马桶中的结块
碳酸钠溶液：用于去除油渍，其原理为酯在碱溶液中水解，参见皂化反应
我要做的两个目的蛋白，分子量一个是140的，另一个是36，内参用的GAPDH。这几天刚刚要做小鼠组织的蛋白，就把新提取的组织蛋白和之前做过的细胞蛋白一起上了，细胞和组织的各上了3个孔。
电泳转膜孵育等条件都是以前做成熟的
之后显影，细胞蛋白的，两个目的和内参都出得挺好；但是小鼠的组织蛋白只有分子量140的那个显出条带了，36的那个显出来的像一片水渍一样，斑片状的黑，内参有时也这样、有时干脆什么都不显。不知道这是什么原因...按说细胞同样的蛋白做出来，WB的各个步骤应该不会有太大问题的。
有点怀疑蛋白提取的不好，我提组织蛋白的的操作是在冰上进行的，裂解液也加有PMSF，用BCA法测得的蛋白浓度大概也有3~5mg/ml。
在这里求助各位高手~望给我指点一下~不胜感激啊！
我打算用HPLC分离已经初步纯化的蛋白，大小40kd到200kd，机器是安捷伦1200.
目前只有C18的柱子，用来分环肽。
如果分比较大的蛋白，有什么效果相对好的柱子，请推荐一下。
在网上见到有人说C18也可以试着分析大蛋白，不知道会不会堵，洗不下来？
