• CompatIBLewithroboticarmautosamplers• Replacescrimptopautosamplervials• Largetargetareaforneedle• UtilizesStepVialdesigntocenterinsertTheR.A.M.TM(roboticarmmachine)vialisspecificallydesignedtoworkwitharoboticarmautosampler.This12x32mmvialhasashortenedscrewthreadarea(9mm),providingalargerspacebetweentheedgeofthecapandtheshoulderofthevialtobegraspedbytheroboticarm.Thisfeaturemakesthevialanexcellentalternativetocrimpcapautosamplervials.TheR.A.M.vialincorporatesaStepVialdesigninsidethevialneckthatallowsprecisecenteringoflimitedvolumeinsertsinthevial,anotherplusforautomatedsystemuse.Thewidemouthopeningis40%largerthanstandardcrimpstylevials.Thislargeropeningprovidesalargertargetareaforneedlepenetrationandhelpspreventbentneedles.ThesevialsfunctionsimilarlytotheKimbleROBOVial,National"sTargetDP,Sun1,andWheaton"sABCvial.
R.A.M.isatrademarkofJ.G.FinneranAssociates,Inc.
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RAW细胞,6孔板,RIPA提取蛋白(提前加好PMAF),每孔100uLRIPA,冰上用tip倒过来刮细胞,放置15min,12000gX15min离心,取上清。做WB时,沸水煮5min,胶冻样物质还是存在。
操作过程中发现离心后还是有胶冻样物质,和上清混在一起无法分开,严重的时候基本上全是胶冻样物质。
这个胶冻样物质应该是DNA,园友提供的方法为用超声碎裂器,但是我们实验室没有。请问还有别的方法可以使用么?
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