Direct quantitative measurements of hydrogen peroxide in biological samples
- Incorporated reference electrode
- Non-breakable integrated hydrogen peroxide sensor with tip dimension of 100 µm
- For use with Apollo1000, Apollo4000, TBR4100 and TBR1025
- Requires cable 91580 (sold separate)
- Package of 3
Options
| Order code | Tip Length | Fiber Diameter | Shape | Package Quantity |
| ISO-HPO-100 | 1-5 mm | 100 µm | Straight | pkg. of 3 |
| ISO-HPO-100H | 5 mm | 100 µm | Hypodermic sheath | |
| ISO-HPO-100-LXX | 1-10 mm | 100 µm | L Shape, Customize length | pkg. of 2 |
- Sensor available in 1 mm length increments (for example, 1 mm, 2mm, 3mm...)- Sensor sensitivity varies with length and diameter.
See the latest Biosensor Data Sheet.
Benefits
- Response Time: < 5 s (90%)
- Detection Limit: 1 nM - 1 mM
- Drift: < 1.0 pA/min.(< 2 pA/min. for the L-Shaped)
- Sensitivity: 1 pA/nM
Applications
- Tissue/Microvessels detection of HPO
- L-Shaped for tissue bath studies
- Hypodermic for easy insertion into tissue
Despite the recognized importance of this oxidant in biology, real-time measurements at low concentration have been difficult. The hydrogen peroxide sensors developed by WPI are designed to compliment existing high sensitivity fluorescent approaches with direct quantitative measurement in biological samples in the low nM range.
The ISO-HPO-2 is a 2.0mm stainless steel sensor, with replaceable membrane sleeves (600012) and an internal refillable electrolyte (100042). It is designed for use in cell cultures and similar applications.
The ISO-HPO-100 is a 100 µm tip diameter hydrogen peroxide micro sensor designed for use in tissues and similar applications. The design is based on a platinum wire sensing electrode coated with a proprietary membrane to enhanceH2O2detection.
These sensors incorporate WPI’s proprietary combination electrode technology whereby the hydrogen peroxide sensing element and separate reference electrode are encased within a single Faraday-shielded probe design. This design has been shown to enhance performance during measurements and minimizes overall sensor size.
Our hydrogen peroxide (H2O2) sensors work with the TBR4100 and TBR1025 free radical analyzers.
Hydrogen peroxide in biological systems
Hydrogen Peroxide is produced in biological systems by controlled pathways at low concentrations that impact on cell signaling. At higher concentrations inflammatory cells produce local intense amounts of this oxidant to kill pathogens. In the progress of human disease, uncontrolled formation of hydrogen peroxide from the mitochondrial respiratory chain and enzymes, such as xanthine oxidase, can occur (Prof. Victor Darley-Usmar, Univ. of Alabama, personal communication).
Hydrogen peroxide sensor in a hypodermic sheath
TheISO-HPO-100His enclosed in a hypodermic needle. The image belowshows the needle tip and enclosed sensor as viewed through a microscope.
L-shaped hydrogen peroxide sensor
TheISO-HPO-100-Lis a unique L-shaped nitric oxide sensor designed specifically for use in tissue bath studies and similar applications (e.g., see WPI"sMYOBATH). The shape of the sensor has been engineered to facilitate placement of the electrode within the lumen of the tissue vessel under study.
ISO-HPO-100 Instruction Manual
| ISO-HPO-100 | ISO-HPO-100 H |
| APPLICATION | Tissue/Microvessels | Hypodermic Sheath |
| SENSOR DIAMETER | 100 µm | 100 µm |
| RESPONSE TIME | < 5 SEC (90%) | < 5 SEC (90%) |
| DETECTION LIMIT | 1 nM to 1 mM | < 10 nM to 1 mM |
| DRIFT | < 2.0 pA/min | < 2.0 pA/min |
| SENSITIVITY | 1 pA/nM | 1 pA/nM |
| PHYSIOLOGICAL INTERFERENCE | Contact WPI | Contact WPI |
Xie, L., Feng, H., Li, S., Meng, G., Liu, S., Tang, X., … Ji, Y. (2016). SIRT3 Mediates the Antioxidant Effect of Hydrogen Sulfide in Endothelial Cells. Antioxidants & Redox Signaling, 24(6), 329–343. http://doi.org/10.1089/ars.2015.6331
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免疫组化多聚物法
immunohistochemistry
英[ɪmjʊnəʊhɪstəʊ'kemɪstrɪ]
美[ɪmjʊnoʊhɪstoʊ'kemɪstrɪ]
[词典]免疫组织化学;
[例句]Methods Transmission electron microscope and immunohistochemistry were used.
方法采用透射电镜和免疫组化法。
苏州高分子科学先进材料有限公司
polymer英[ˈpɒlɪmə(r)]美[ˈpɑ:lɪmə(r)]
n.多聚物; [高分子] 聚合物;
[例句]The nature of the polymer is currently a trade secret.
这一聚合物的性质目前是个商业机密。
[其他]复数：polymers
英 [ˈpɒlɪmə(r)]
美 [ˈpɑ:lɪmə(r)]
n.
多聚物; [高分子] 聚合物;
[例句]The nature of the polymer is currently a trade secret.
这一聚合物的性质目前是个商业机密。
[其他]
复数：polymers
高分子化合物又称高分子（聚合物），高分子是由分子量很大的长链分子所组成，高分子的分子量从几千到几十万甚至几百万。而每个分子链都是由共价键联合的成百上千的一种或多种小分子构造而成。高分子的分类有多种，按来源可分为天然高分子、天然高分子衍生物、合成高分子三大类；根据用途则可分为合成树脂和塑料、合成橡胶、合成纤维等；按热行为可分为热塑性和热固性聚合物；按主链结构可分为碳链、杂链、和元素有机三类；另外根据工业产量和价格还可分为通用高分子、中间高分子、工程塑料以及特种高分子等等。
