
Recombinant Human His6-UBE2G2 Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E2-680
Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP |
Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: UBE2G2
Ubiquitin-conjugating Enzyme E2G 2 (UBE2G2), also known as Ubiquitin-conjugating Enzyme 7 (Ubc7), is a ubiquitously expressed member of the Ubiquitin-conjugating (E2) enzyme family that localizes to both the nucleus and cytoplasm (1,2). UBE2G2 has a predicted molecular weight of 18 kDa. The human protein shares 100% amino acid sequence identity with the mouse and rat orthologs. UBE2G2 interacts with the HRD1 and gp78 Ubiquitin ligases (E3s) to mediate efficient endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins in human cells (3,4). Mechanistically, it has been shown that UBE2G2 forms Lys48-linked Ubiquitin chains on itself prior to gp78-dependent transfer of the Ubiquitin chains to target proteins (5,6). UBE2G2 also interacts with the CRL4(Cdt2) E3 complex to promote the ubiquitination and degradation of Cdt1 following UV irradiation, suggesting that UBE2G2 may function in the response to DNA damage (7). This protein has an N-terminal His6-tag. (Accession # NP_003334)
- Katsanis, N. & E.M. Fisher (1998) Genomics 51:128.
- Napolitano, L.M. et al. (2011) Biochem. J. 434:309.
- Kikkert, M. et al. (2004) J. Biol. Chem. 279:3525.
- Chen, B. et al. (2006) Proc. Natl. Acad. Sci. USA 103:341.
- Li, W. et al. (2007) Nature 446:333.
- Li, W. et al. (2009) Proc. Natl. Acad. Sci. USA 106:3722.
- Shibata, E. et al. (2011) Mol. Cell. Biol. 31:3136.
Citation for Recombinant Human His6-UBE2G2 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
1Citation: Showing 1 - 1
- Phosphorylation of Kif26b Promotes Its Polyubiquitination and Subsequent Proteasomal Degradation during Kidney Development.Authors: Terabayashi T, Sakaguchi M, Shinmyozu KPLoS ONE, 2012;7(6):e39714.
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英 [ˈpɒlɪmə(r)]
美 [ˈpɑ:lɪmə(r)]
n.
多聚物; [高分子] 聚合物;
[例句]The nature of the polymer is currently a trade secret.
这一聚合物的性质目前是个商业机密。
[其他]
复数:polymers
高分子化合物又称高分子(聚合物),高分子是由分子量很大的长链分子所组成,高分子的分子量从几千到几十万甚至几百万。而每个分子链都是由共价键联合的成百上千的一种或多种小分子构造而成。高分子的分类有多种,按来源可分为天然高分子、天然高分子衍生物、合成高分子三大类;根据用途则可分为合成树脂和塑料、合成橡胶、合成纤维等;按热行为可分为热塑性和热固性聚合物;按主链结构可分为碳链、杂链、和元素有机三类;另外根据工业产量和价格还可分为通用高分子、中间高分子、工程塑料以及特种高分子等等。
Author:AndrewJ.Hall,MilenaQuaglia,PanagiotisManesiotis,ErsiliaDeLorenzi,andBörjeSellergren
Resource:AnalyticalChemistry,2006,78(24):8362-8367.
Abstract:
Arangeof2-acrylamidopyridines,showingsubtledifferencesinsolutionbindingtowardcarboxylicacids,hasbeeninvestigatedasfunctionalmonomersinmolecularimprinting.ImprintingofN-Z-L-glutamicacidwithonesuchmonomerisshowntobeeffectiveinthecreationofamolecularlyimprintedpolymer(MIP)withrecognitionpropertiesforitstemplateandalsoforlargermoleculescontainingglutamicacidresidues.IncomparisontoaMIPpreparedviaamore"trADItional"approach,thenewpolymericreceptorsexhibitreducednonspecificbinding.Thenewreceptorsarecomparedwithpreviouslyreportedurea-basedreceptorstargetingtheglutamicacidresidueandreceptorstargetingthepteridinesubstructureoffolicacid.
PMID:17165828
苏州高分子科学先进材料有限公司
polymer英[ˈpɒlɪmə(r)]美[ˈpɑ:lɪmə(r)]
n.多聚物; [高分子] 聚合物;
[例句]The nature of the polymer is currently a trade secret.
这一聚合物的性质目前是个商业机密。
[其他]复数:polymers
免疫组化多聚物法
immunohistochemistry
英[ɪmjʊnəʊhɪstəʊ'kemɪstrɪ]
美[ɪmjʊnoʊhɪstoʊ'kemɪstrɪ]
[词典]免疫组织化学;
[例句]Methods Transmission electron microscope and immunohistochemistry were used.
方法采用透射电镜和免疫组化法。

