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Materials Blot Cell BA 83 0.2 µ m pore nitrocellulose sheets Buffer, PBS-Tween 20 Antigenic proteins, antibodies, and horseradish peroxidase labeled anti
WESTERN BLOTTING USING CHEMILUMINESCENCE Western Blotting...
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REAGENTS ECL Western blotting kit (Amersham Life Science; cat# RPN2108): contains second antibodies for both mouse and rabbit, substrate and milk blocker (the
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For Protein Concentration Determination of Cell CultureDecant medium from 10cm dish of adherent cells and rinse plate rapidly with phosphate-buffered saline (P
D band18
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For Protein Concentration Determination of Cell CultureDecant medium from 10cm dish of adherent cells and rinse plate rapidly with phosphate-buffered saline (P
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Western Blotting Using the SemiPhor Semi-Dry Transfer Unit Remove the stacking gel from the gel. Measure the gel and record its size. Do not pre-soak the gel.
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Studying Protein Interactions by Far-Western BlottingFar-Western blotting was originally developed to screen protein expression libraries with 32P-labeled glut
HPLC测定辅酶Q10软胶囊中的主药并检查有关物质论文万方医学网
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Preparation of Nuclear Extracts for Gel Shifts and Westerns BlotsThe following protocol is optimized for about 107 cells (near confluent 10 cm plate). NOTE: AL
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Isolation of Protein from TissuePlace the tissues on labeled aluminum foil and immediately place in dry ice. It is imperative that the tissues stay cold so tha
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