SodiumCitrateAntigenRetrieval:
1.Placeslidesinaglassslideholderandfillintherestofthe
rackwithblankslides(10total)toensureevenheating.
2.Placerackin600mlof10mMsodiumcitrate,pH6.0ina
glass2L-beaker.Markalineatthetopoftheliquidonthe
beaker.
3.Microwavefor20mintotal,replacingevaporatedwater
every5min.
4.Coolslidesfor20min.
5.Wash4x3mininddH2O,and3minin1XPBS
ProteinaseKAntigenRetrieval:
1.Makeafreshsolutionof:
25ulof20mg/mlProteinaseK
2.5mlof1MTris-Cl,pH8.0
0.5mlof0.5MEDTA,pH8.0
qs50mlwithddH2O
2.Incubateslidesinaglassslideholderwithsolutionat37ºC
for5min(donotpre-warmProteinaseKsolution).
3.Wash3x5minwith1XPBS.
UreaAntigenRetrieval:
1.Makeafreshsolutionof1Murea.
2.Placeslidesinaglassslideholderandfillintherestofthe
rackwithblankslides(10total)toensureevenheating.
3.Placerackin600mlof1Mureainaglass2L-beaker.Mark
alineatthetopoftheliquidonthebeaker.
4.Microwavefor10,20or30mintotal,replacingevaporated
waterevery5min.
5.Coolslidesfor30minto1hr.
6.Wash4x3mininddH2O,and3minin1XPBS.
