| Product Type | Proteins & Peptides |
| Units | 100 mg |
Background Purified rabbit albumin may be used as a reference antigen, calibrator, coating protein and blocking agent in a variety of immunoassays including immunodiffusion, immunoelectrophoresis, ELISA, Western blotting, dot-immunobinding assays (DIBA), haemagglutination, and cellbinding assays.
ProductLyophilized, stable white powder. No preservative added. After opening the vial, the lyophilized content is reconstituted by adding 1 ml of sterile distilled water, mixed gently by inversion until complete dissolution is obtained.
Formulation: Lyophilized, stable white powder. No preservative added.
SpecificityChromatographically purified Rabbit albumin.
Applications
StorageThis product is shipped lyophilized at ambient temperature. After arrival store at +4°C. Prolonged storage may be at -20°C.Allow to stand at ambient temperature for 5-10 minutes to reach equilibrium. Reconstituted solution may be stored frozen. Working dilutions may be prepared by adding the required amount of phosphate buffered saline (PBS), should not be refrozen and preferably used within 24 hours. Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product.
CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
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多抗，稀释度更大，特异性相对较差，容易出现多条带。
兔的单克隆抗体和鼠的单克隆抗体在使用上不会有什么区别。
用来很多抗体，许多时候觉得单抗多抗也未必是理论上那样的。单抗做不好的也有，多抗条带唯一且清晰的也有。
而且很多蛋白的抗体未必有那么多的选择。
一般来说多克隆的阳性率高一些，但出现假阳性的比例也高一些。
1取组织
（1） 灌注固定：暴露心脏,将注射针头插入左心室,剪开右心耳,快速灌注PBS,待右心耳处流出澄清液体,换4%多聚甲醛灌注到大鼠四肢强直为止,快速取脑和肾。
（2） 固定：将组织置于4%多聚甲醛中固定15小时
（3） 脱水：将组织放入30% 蔗糖溶液中直至下沉
（4） 切片：脑组织冰冻切片20微米，37度烘干2h以上；
（5）将切片放置－80度保存，随用随取。
2免疫单标记
（1）做抗原修复（0.4g柠檬酸、3g柠檬酸钠、1000ml水配制成溶液，放到水浴箱中90~94℃保存15min）,冷却后， 用1*PBS反复洗标本10min，吸去PBS，同时向标本加渗透液（配0.4%trition液，然后将BSA加入到配好的trition液中，配成1％浓度）100ul，常温下湿盒反应1h。
（2） 吸去渗透液，同时向标本加block液（5％封闭用正常山羊血清）100ul，常温下湿盒反应5h。
（3） 吸去block液，同时向标本加入1：50稀释的羊抗鼠一抗100ul，37度湿盒反应过夜。
（4）吸去一抗，并回收，用1*PBS洗液反复洗15min。
（5）快速从冰箱中取出驴抗羊的二抗，并立即用锡纸包裹，注入到一个新的以锡纸包裹的tube管中并用PBS以1：200稀释。
（6）进入暗室，吸去PBS，同时加入二抗100ul，37度湿盒反应2h,封片。
（7）放置到荧光显微镜下观察。
不过多克隆抗体因为有多个结合位点，事实上的特异性并不如单克隆抗体，所以一般Western blot都尽量要求使用单克隆抗体。以保证实验结果准确可靠
