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eEnzyme是一家美国高质量生物学试剂及检测试剂盒品牌供应商,其与美国国立卫生研究院合作紧密,在全球范围内为广大科研客户提供高品质的产品与技术服务,其主要生产销售高质量的抗体和用于传染病研究的抗体/抗原,细胞因子蛋白,pcr产品等用于分子和细胞生物学研究的生物试剂,产品应用于生物技术研究,制药,药物发现,癌症研究,传染性疾病,微生物和分子诊断等众多生命科学技术领域
eENZYME为科学界生产和销售高质量的生物试剂盒和生物分析试剂盒。与NIH密切合作以保证产品在生命科学研究的多个领域成功地使用。努力提供新颖价廉且有效的工具,使实验更容易成功。产品在科研、生物技术、制药和政府的研究实验室中得到广泛的应用,包括药物发现、癌症研究、传染病研究、微生物学和分子诊断。
【简单介绍】eENZYME生产销售高质量的抗体和用于传染病研究的抗体/抗原,细胞因子蛋白,PCR产品等用于分子和细胞生物学研究的生物试剂,产品应用于生物技术研究,制药,药物发现,癌症研究,传染性疾病,微生物和分子诊断等众多生命科学技术领域。【详细说明】欢迎eENZYMEeENZYME生产销售高质量的抗体和用于传染病研究的抗体/抗原,细胞因子蛋白,PCR产品等用于分子和细胞生物学研究的生物试剂,产品应用于生物技术研究,制药,药物发现,癌症研究,传染性疾病,微生物和分子诊断等众多生命科学技术领域。 Ë ENZYMEis的高品质生物试剂及实验室设备科学界一个制造商和分销商。我们密切合作,与美国国立卫生研究院的社区,以保证我们的产品生命科学研究在广泛领域的成功表现。我们致力于提供创新和具有成本效益的工具,使您更容易取得成功。我们的产品用于在学术,生物技术,制药和政府研究实验室,包括药物发现,癌症研究,传染病研究,微生物学和分子诊断许多不同的应用。公司网站:www.eenzyme.com 目录号描述产品类型应用单位供应商IA-01SW-0100抗HA(A/California/06/09)(甲型H1N1流感)兔多克隆抗体WB等100微克eENZYMEIA-011-0100抗H1(H1N1/Pan)兔多克隆抗体WB等100微克eENZYMEIA-001-0100抗HA1(A/新喀里多尼亚)(甲型H1N1流感)兔多克隆抗体WB等100微克eENZYMEIA-023-0100抗-NP(A/新Caledonia/20/1999)(甲型H1N1流感兔多克隆抗体WB等100微克eENZYMEIA-022-0100抗M2型(H1N1/A/新喀里多尼亚(H1N1))兔多克隆抗体WB等100微克eENZYMEIA-004A-0100抗-H3(H3N2)(A/WIS/2005)兔多克隆抗体WB等100微克eENZYMEIA-003-0100抗-M1(H3N2)[A/Wyoming/3/03)兔多克隆抗体WB等100微克eENZYMEIA-004-0100抗HA(H3N2)[A/Wyoming/3/03)兔多克隆抗体WB等100微克eENZYMEIA-020-0100抗-NA(H3N2)[A/Wyoming/3/03)兔多克隆抗体WB等100微克eENZYMEIA-005H-0100抗H5(A/香港Kong/483/97)(H5N1)兔多克隆抗体WB等100微克eENZYMEIA-005-0100抗HA1(H5N1)(A/Vietnam/1203/2004)兔多克隆抗体WB等100微克eENZYMEIA-006-0100抗HA1(H5N1,禽流感)(A/斑头雁/Qinghai/12/05)兔多克隆抗体WB等100微克eENZYMEIA-010-0100抗-HA2(A/Vietnam/1203/2004)(H5N1)兔多克隆抗体WB等100微克eENZYMEIA-012-0100抗-M1(A/Vietnam/1203/2004)(H5N1)兔多克隆抗体WB等100微克eENZYMEIA-021-0100抗-NA(A/Vietnam/1203/2004)(H5N1,人)兔多克隆抗体WB等100微克eENZYMEIA-025-0100抗NS1(A/Vietnam/1203/2004)(H5N1)兔多克隆抗体WB等100微克eENZYMEIA-003-0100抗HA1(A/Chicken/NY/29878/91)(H2N2)兔多克隆抗体WB等100微克eENZYMEIA-009-0100抗HA1(A/chicken/HongKong/NT366/03)(H9N2)兔多克隆抗体WB等100微克eENZYMEIA-027-0100抗-NA(A/Chicken/HongKong/NT366/03)(H9N2)兔多克隆抗体WB等100微克eENZYMEIA-007-0100抗HA1(A/duck/Hainan/6/2004)(H6N2)兔多克隆抗体WB等100微克eENZYMEIA-008-0100抗HA1(A/鸡/荷兰/2/03)兔多克隆抗体WB等100微克eENZYMEIA-028-0100抗-NA(A/Chicken/Netherlands/1/03)(H7N7)兔多克隆抗体WB等100微克eENZYMEIA-0015-0100抗-H11(A/duck/Yangzhou/906/02)(H11N2)兔多克隆抗体WB等100微克eENZYMEIA-003-0031抗B型流感(NP)的多克隆兔多克隆抗体WB等100微克eENZYMEIA-003-0032抗B型流感(医管局)多克隆兔多克隆抗体WB等100微克eENZYMEIA-013-0100抗-H12(A/pintail/Alaska/102/05)(H12N5)兔多克隆抗体WB等100微克eENZYMEIA-014-0100抗-H4(A/environment/Maryland/1101/06)(H4N6)兔多克隆抗体WB等100微克eENZYMEIA-003-018抗-H15(A/Australia/83/00(H15N8))兔多克隆抗体WB等100微克eENZYMEIA-003-019抗-H16(A/Sweden/99(H16N3))兔多克隆抗体WB等100微克eENZYME目录号描述产品类型应用单位供应商IA-01SW-0100抗HA(A/California/06/09)(甲型H1N1流感)兔多克隆抗体WB等100微克eENZYMEIA-011-0100抗H1(H1N1/Pan)兔多克隆抗体WB等100微克eENZYMEIA-001-0100抗HA1(A/新喀里多尼亚)(甲型H1N1流感)兔多克隆抗体WB等100微克eENZYMEIA-023-0100抗-NP(A/新Caledonia/20/1999)(甲型H1N1流感兔多克隆抗体WB等100微克eENZYMEIA-022-0100抗M2型(H1N1/A/新喀里多尼亚(H1N1))兔多克隆抗体WB等100微克eENZYMEIA-004A-0100抗-H3(H3N2)(A/WIS/2005)兔多克隆抗体WB等100微克eENZYMEIA-003-0100抗-M1(H3N2)[A/Wyoming/3/03)兔多克隆抗体WB等100微克eENZYMEIA-004-0100抗HA(H3N2)[A/Wyoming/3/03)兔多克隆抗体WB等100微克eENZYMEIA-020-0100抗-NA(H3N2)[A/Wyoming/3/03)兔多克隆抗体WB等100微克eENZYMEIA-005H-0100抗H5(A/香港Kong/483/97)(H5N1)兔多克隆抗体WB等100微克eENZYMEIA-005-0100抗HA1(H5N1)(A/Vietnam/1203/2004)兔多克隆抗体WB等100微克eENZYMEIA-006-0100抗HA1(H5N1,禽流感)(A/斑头雁/Qinghai/12/05)兔多克隆抗体WB等100微克eENZYMEIA-010-0100抗-HA2(A/Vietnam/1203/2004)(H5N1)兔多克隆抗体WB等100微克eENZYMEIA-012-0100抗-M1(A/Vietnam/1203/2004)(H5N1)兔多克隆抗体WB等100微克eENZYMEIA-021-0100抗-NA(A/Vietnam/1203/2004)(H5N1,人)兔多克隆抗体WB等100微克eENZYMEIA-025-0100抗NS1(A/Vietnam/1203/2004)(H5N1)兔多克隆抗体WB等100微克eENZYMEIA-003-0100抗HA1(A/Chicken/NY/29878/91)(H2N2)兔多克隆抗体WB等100微克eENZYMEIA-009-0100抗HA1(A/chicken/HongKong/NT366/03)(H9N2)兔多克隆抗体WB等100微克eENZYMEIA-027-0100抗-NA(A/Chicken/HongKong/NT366/03)(H9N2)兔多克隆抗体WB等100微克eENZYMEIA-007-0100抗HA1(A/duck/Hainan/6/2004)(H6N2)兔多克隆抗体WB等100微克eENZYMEIA-008-0100抗HA1(A/鸡/荷兰/2/03)兔多克隆抗体WB等100微克eENZYMEIA-028-0100抗-NA(A/Chicken/Netherlands/1/03)(H7N7)兔多克隆抗体WB等100微克eENZYMEIA-0015-0100抗-H11(A/duck/Yangzhou/906/02)(H11N2)兔多克隆抗体WB等100微克eENZYMEIA-003-0031抗B型流感(NP)的多克隆兔多克隆抗体WB等100微克eENZYMEIA-003-0032抗B型流感(医管局)多克隆兔多克隆抗体WB等100微克eENZYMEIA-013-0100抗-H12(A/pintail/Alaska/102/05)(H12N5)兔多克隆抗体WB等100微克eENZYMEIA-014-0100抗-H4(A/environment/Maryland/1101/06)(H4N6)兔多克隆抗体WB等100微克eENZYMEIA-003-018抗-H15(A/Australia/83/00(H15N8))兔多克隆抗体WB等100微克eENZYMEIA-003-019抗-H16(A/Sweden/99(H16N3))兔多克隆抗体WB等100微克eENZYME目录号描述产品类型应用单位供应商IA-01SW-0100抗HA(A/California/06/09)(甲型H1N1流感)兔多克隆抗体WB等100微克eENZYMEIA-011-0100抗H1(H1N1/Pan)兔多克隆抗体WB等100微克eENZYMEIA-001-0100抗HA1(A/新喀里多尼亚)(甲型H1N1流感)兔多克隆抗体WB等100微克eENZYMEIA-023-0100抗-NP(A/新Caledonia/20/1999)(甲型H1N1流感兔多克隆抗体WB等100微克eENZYMEIA-022-0100抗M2型(H1N1/A/新喀里多尼亚(H1N1))兔多克隆抗体WB等100微克eENZYMEIA-004A-0100抗-H3(H3N2)(A/WIS/2005)兔多克隆抗体WB等100微克eENZYMEIA-003-0100抗-M1(H3N2)[A/Wyoming/3/03)兔多克隆抗体WB等100微克eENZYMEIA-004-0100抗HA(H3N2)[A/Wyoming/3/03)兔多克隆抗体WB等100微克eENZYMEIA-020-0100抗-NA(H3N2)[A/Wyoming/3/03)兔多克隆抗体WB等100微克eENZYMEIA-005H-0100抗H5(A/香港Kong/483/97)(H5N1)兔多克隆抗体WB等100微克eENZYMEIA-005-0100抗HA1(H5N1)(A/Vietnam/1203/2004)兔多克隆抗体WB等100微克eENZYMEIA-006-0100抗HA1(H5N1,禽流感)(A/斑头雁/Qinghai/12/05)兔多克隆抗体WB等100微克eENZYMEIA-010-0100抗-HA2(A/Vietnam/1203/2004)(H5N1)兔多克隆抗体WB等100微克eENZYMEIA-012-0100抗-M1(A/Vietnam/1203/2004)(H5N1)兔多克隆抗体WB等100微克eENZYMEIA-021-0100抗-NA(A/Vietnam/1203/2004)(H5N1,人)兔多克隆抗体WB等100微克eENZYMEIA-025-0100抗NS1(A/Vietnam/1203/2004)(H5N1)兔多克隆抗体WB等100微克eENZYMEIA-003-0100抗HA1(A/Chicken/NY/29878/91)(H2N2)兔多克隆抗体WB等100微克eENZYMEIA-009-0100抗HA1(A/chicken/HongKong/NT366/03)(H9N2)兔多克隆抗体WB等100微克eENZYMEIA-027-0100抗-NA(A/Chicken/HongKong/NT366/03)(H9N2)兔多克隆抗体WB等100微克eENZYMEIA-007-0100抗HA1(A/duck/Hainan/6/2004)(H6N2)兔多克隆抗体WB等100微克eENZYMEIA-008-0100抗HA1(A/鸡/荷兰/2/03)兔多克隆抗体WB等100微克eENZYMEIA-028-0100抗-NA(A/Chicken/Netherlands/1/03)(H7N7)兔多克隆抗体WB等100微克eENZYMEIA-0015-0100抗-H11(A/duck/Yangzhou/906/02)(H11N2)兔多克隆抗体WB等100微克eENZYMEIA-003-0031抗B型流感(NP)的多克隆兔多克隆抗体WB等100微克eENZYMEIA-003-0032抗B型流感(医管局)多克隆兔多克隆抗体WB等100微克eENZYMEIA-013-0100抗-H12(A/pintail/Alaska/102/05)(H12N5)兔多克隆抗体WB等100微克eENZYMEIA-014-0100抗-H4(A/environment/Maryland/1101/06)(H4N6)兔多克隆抗体WB等100微克eENZYMEIA-003-018抗-H15(A/Australia/83/00(H15N8))兔多克隆抗体WB等100微克eENZYMEIA-003-019抗-H16(A/Sweden/99(H16N3))兔多克隆抗体WB等100微克eENZYME
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多抗,稀释度更大,特异性相对较差,容易出现多条带。
兔的单克隆抗体和鼠的单克隆抗体在使用上不会有什么区别。
用来很多抗体,许多时候觉得单抗多抗也未必是理论上那样的。单抗做不好的也有,多抗条带唯一且清晰的也有。
而且很多蛋白的抗体未必有那么多的选择。
抗原有两个基本特性,即抗原性和免疫原性。有抗原性的物质不一定有免疫原性,所以由此引出半抗原和完全全抗原,半抗原必须经过经过一定的改造(偶联蛋白载体BSA,OVA或者HSA等大分子物质)方能成为完全。一般而言完全抗原分子量越大(大于10KDa),结构越复杂引起免疫反应的能力也就越强。
抗体就是能与特异性抗原结合的免疫球蛋白,抗体一般分为多克隆抗体和单克隆抗体,多克隆抗体能与抗原的多个表位结合。本篇主要讲述兔来源的多克隆抗体的生产步骤
多抗一般制备流程:完全抗原的准备→兔子的免疫→ 效价检测和终放→抗体亲和纯化→抗体的浓缩和保存。
一般来说多克隆的阳性率高一些,但出现假阳性的比例也高一些。
抗体的特异性鉴定 抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在 IC50时的浓度,并按下列公式计算交叉反应率。 如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示 这一抗血清与其他抗原物质的交叉反应率近似为 0,即该血清的特异性较好。
抗体的亲和力 是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小、抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键、疏水键、侧链相反电荷基因的库仑力、范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol,通常K的范围在 108 ~1010 /mol,也有多达 1014 /mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。向左转|向右转

