Polyclonal Antibody to Interferon Alpha (IFNa)
IFNA1; IFL; LeIF D; IFN; IFN-Alpha; IFNA13; IFN-A; IFNAP22; IFN-Alpha 1b; Interferon Alpha 1b; Interferon, Leukocytic; IFN, Leukocyte; Interferon alpha-D
- Product No.PAA033Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig TypeIgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- ImmunogenRPA033Ra01-Recombinant Interferon Alpha (IFNa)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species MoreHomo sapiens (Human)
- ApplicationsWB; IHC; ICC; IP.If the antibody is used in flow cytometry, please check FCM antibodies.
- DownloadInstruction Manual
- UOM20µl100µl200µl1ml10ml
- FOBUS$ 103 For more details, please contact local distributors!US$ 239 For more details, please contact local distributors!US$ 342 For more details, please contact local distributors!US$ 855 For more details, please contact local distributors!US$ 3420 For more details, please contact local distributors!
SPECIFITY of the Polyclonal Antibody to Interferon Alpha (IFNa)
The antibody isa rabbit polyclonal antibody raised against IFNa. It has been selected for its ability to recognize IFNa in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Interferon Alpha (IFNa)
Western blotting: 0.5-2µg/mL;Immunohistochemistry: 5-20µg/mL;Immunocytochemistry: 5-20µg/mL;Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Interferon Alpha (IFNa)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Interferon Alpha (IFNa)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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INCREMENT SERVICES
Antibody Labeling Customized ServiceProtein A/G Purification ColumnStaining Solution for Cells and TissuePositive Controlfor AntibodyTissue/Sections Customized ServicePhosphorylated Antibody Customized ServiceWestern Blot (WB) Experiment ServiceImmunohistochemistry (IHC) Experiment ServiceImmunocytochemistry (ICC) Experiment ServiceFlow Cytometry (FCM) Experiment ServiceImmunoprecipitation (IP) Experiment ServiceImmunofluorescence (IF) Experiment ServiceBufferDAB Chromogen KitSABC KitLong-arm Biotin Labeling KitReal Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Rattus norvegicus (Rat) | Applications (RESEARCH USE ONLY!) |
| APA033Ra01 | Active Interferon Alpha (IFNa) | Cell culture; Activity Assays. |
| EPA033Ra61 | Eukaryotic Interferon Alpha (IFNa) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA033Ra01 | Recombinant Interferon Alpha (IFNa) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA033Ra61 | Active Interferon Alpha (IFNa) | Cell culture; Activity Assays. |
| PAA033Ra01 | Polyclonal Antibody to Interferon Alpha (IFNa) | WB; IHC; ICC; IP. |
| PAA033Ra06 | Polyclonal Antibody to Interferon Alpha (IFNa) | WB; IHC; ICC; IP. |
| FAA033Ra01 | Anti-Interferon Alpha (IFNa) Polyclonal Antibody | Flow cytometry. |
| MAA033Ra21 | Monoclonal Antibody to Interferon Alpha (IFNa) | WB; IHC; ICC; IP. |
| MAA033Ra26 | Monoclonal Antibody to Interferon Alpha (IFNa) | WB; IHC; ICC; IP. |
| SEA033Ra | ELISA Kit for Interferon Alpha (IFNa) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| IEA033Ra | Instant ELISA Kit for Interferon Alpha (IFNa) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA033Ra | CLIA Kit for Interferon Alpha (IFNa) | Chemiluminescent immunoassay for Antigen Detection. |
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其次,察看次目的蛋白的存在形式,有没有多聚体形式及变构形式;
最后,查看多家抗体公司的DATA,看看别人的WB做出来的条带的位置。
根据你说的,特异识别多个组织中的同样大小的条带,我觉得很可能就是你的目的蛋白。
一般来说多克隆的阳性率高一些,但出现假阳性的比例也高一些。
1取组织
(1) 灌注固定:暴露心脏,将注射针头插入左心室,剪开右心耳,快速灌注PBS,待右心耳处流出澄清液体,换4%多聚甲醛灌注到大鼠四肢强直为止,快速取脑和肾。
(2) 固定:将组织置于4%多聚甲醛中固定15小时
(3) 脱水:将组织放入30% 蔗糖溶液中直至下沉
(4) 切片:脑组织冰冻切片20微米,37度烘干2h以上;
(5)将切片放置-80度保存,随用随取。
2免疫单标记
(1)做抗原修复(0.4g柠檬酸、3g柠檬酸钠、1000ml水配制成溶液,放到水浴箱中90~94℃保存15min),冷却后, 用1*PBS反复洗标本10min,吸去PBS,同时向标本加渗透液(配0.4%trition液,然后将BSA加入到配好的trition液中,配成1%浓度)100ul,常温下湿盒反应1h。
(2) 吸去渗透液,同时向标本加block液(5%封闭用正常山羊血清)100ul,常温下湿盒反应5h。
(3) 吸去block液,同时向标本加入1:50稀释的羊抗鼠一抗100ul,37度湿盒反应过夜。
(4)吸去一抗,并回收,用1*PBS洗液反复洗15min。
(5)快速从冰箱中取出驴抗羊的二抗,并立即用锡纸包裹,注入到一个新的以锡纸包裹的tube管中并用PBS以1:200稀释。
(6)进入暗室,吸去PBS,同时加入二抗100ul,37度湿盒反应2h,封片。
(7)放置到荧光显微镜下观察。
又由于自然存在的抗原大都存在多个抗原表位,会刺激机体产生多种针对同一抗原的不同抗原表位相应的不同抗体.


