| Clone | C1P5 |
| Isotype | IgG1 |
| ProductType | MonoclonalAntibodyPrimaryAntibodies |
| Units | 3ml |
| Host | Mouse |
| Application | Immunohistochemistry(frozen¶ffin)Immunohistochemistry(frozen)Immunohistochemistry(paraffin) |
BackgroundThisantibodyisusedforthedetectionofhighriskHumanPapillomavirus(HPV)infectionsindifferenttissues.ItshouldalwaysbeusedwhenthescreeningantibodyhasdetectedthepresenceofpapillomavirusinsUSPectedtissues.HumanPapillomavirustype16and18areresponsIBLeforthedevelopmentofcervicalcarcinoma,aswellascarcinomasoftheheadandneckregionandsomeothertumors.
SourceMouseimmunizedwithrecombinantE6proteinfroHPV18
Immunogen:HPVtype18E6proteinproducedinbacteria
ProductAntibodysolutioninstABIlizingphosphatebufferpH7.3.Contains0.09%sodiumazide**.Thevolumeissufficientforatleast30immunohistochemicaltests(100µlworkingsolution/test).Useappropriateantibodydiluente.g.BIOLOGOArt.No.PU002ifrequired.
PurificationMethod:AntibodysolutioninstabilizingphosphatebufferpH7.3.Contains0.09%sodiumazide**.Thevolumeissufficientforatleast30immunohistochemicaltests(100µlworkingsolution/test).Useappropriateantibodydiluente.g.BIOLOGOArt.No.PU002ifrequired.
SecondaryReagents:WerecommendtheuseofBIOLOGO"sUniversalStainingSystemDAB(Art.No.DA005)orAEC(Art.-No.AE005).
SpecificityHumanPapillomavirusEarlyAntigenE6fromHPV16and18.
ApplicationsIHC(C,P)
IncubationTime:60minatRT
WorkingConcentration:(RTU)neat
Pre-Treatment:ImprovementofstainingwithunmaskingfluidG(Art.No.DE007)orunmaskingfluidC(Art.No.DE000)
PositiveControl:HPV-positivevulvacarcinoma
Storage2-8°C
CautionThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Itmaycontainhazardousingredients. PleaserefertotheSafetyDataSheets(SDS)foradditionalinformationandproperhandlingprocedures.Disposeproductremaindersaccordingtolocalregulations.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-Mubioacceptsnoliabilityforanyinaccuraciesoromissionsinthisinformation.
References1.Cowsertetal.(1987)J.Natl.CancerInst.79;1053-1057.2.BanksL.J.(1987)J.Gen.Virol.68;1351-1355.3.CasonJ.,PatelD.,NaylorJ.,etal.(1989)Identificationofimmunogenicregionsofthemajorcoatproteinofhumanpapillomavirustype16thatcontaintype-restrictedepitopes.J.GeneralVirol.70;2973-2987.4.ShepherdP.S.,LunnyD.,BrooksR.,etal.(1992)Thedetectionofhumanpapillomavirusesincervicalbiopsiesbyimmunohistochemistryandinsituhybridization.Scand.J.Immunol.36(11);69-74.
SafetyDatasheet(s)forthisproduct:NM_SodiumAzide/wp-content/uploads/SDS/AntibodySDSwithSodiumAzideNoridic-MUbio.pdf
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制备抗体都需要进行纯化处理
又由于自然存在的抗原大都存在多个抗原表位,会刺激机体产生多种针对同一抗原的不同抗原表位相应的不同抗体.
清冷冻干燥后保存。
一般来说多克隆的阳性率高一些，但出现假阳性的比例也高一些。
抗体的特异性鉴定 抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高，它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线，计算各自的结合率，求出各自在 IC50时的浓度，并按下列公式计算交叉反应率。 如果所用抗原浓度IC50浓度为pg/管，而一些近似抗原物质的IC50浓度几乎是无穷大时，表示 这一抗血清与其他抗原物质的交叉反应率近似为 0，即该血清的特异性较好。
抗体的亲和力 是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小、抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键、疏水键、侧链相反电荷基因的库仑力、范德华力和空间斥力。亲和力常以亲和常数K表示，K的单位是L/mol，通常K的范围在 108 ～1010 /mol，也有多达 1014 /mol。抗体亲和力的测定对抗体的筛选，确定抗体的用途，验证抗体的均一性等均有重要意义。向左转|向右转
