Type
PolyclonalAntibody
Applications
Westernblotting,ELISA
SourceofAntigen
E.coli
Hosts
Rabbit
Preparation
TheantibodywasraisedinrabbitsbyimmunizationwiththerecombinantRatClusterin.
AminoAcidSequence
Theimmunizationantigen(26.5 kDa)isaproteincontaining240 AAofrecombinantRatClusterin.N-TerminalT7-TagandC-TerminalHis-tag,25 extraAA(highlighted).
MASMTGGQQMGRDPNSSSPFYFWMNGDRIDSLLESDRQQSQVLDAMQDSFTRASGIIDTLFQDRFFTHEPQDIHHFSPMGFPHKRPHLLYPKSRLVRSLMPLSHYGPLSFHNMFQPFFDMIHQAQQAMDVQLHSPALQFPDVDFLKEGEDDRTVCKEIRHNSTGCLKMKGQCEKCQEILSVDCSTNNPAQANLRQELNDSLQVAERLTQQYNELLHSLQSKMLNTSSLLEQALEHHHHHH
TheaminoacidsequenceoftherecombinantRatClusterinis100%homologoustotheaminoacidsequence(AA146–360)oftheRatClusterinprecursor
PurificationMethod
ImmunoaffinitychromatographyonacolumnwithimmobilizedrecombinantRatClusterin.
AntibodyContent
0.1 mg(determinedbyBCAmethod,BSAwasusedasastandard)
Formulation
Theantibodyislyophilizedin0.05 Mphosphatebuffer,0.1 MNaCl,pH7.2. AZIDEFREE.
Reconstitution
Weadviseyoutocentrifugethisproductvialbeforeuse.Add0.1 mlofdeionizedwater,mixgentlyandletthelyophilizedpelletdissolvecompletely(atleast15 minuteswithoccasionallygentlyshaking,nottofoam).Slightturbiditymayoccurafterreconstitution,whichdoesnotaffectactivityoftheantibody.Inthiscaseclarifythesolutionbycentrifugation.
Storage/Expiration
Thelyophilizedantibodyremainsstableandfullyactiveuntiltheexpirydatewhenstoredat –20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcyclesandstorefrozenat –80°C.Reconstitutedantibodycanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowdeclineinactivityafteroneweekat 4°C.
QualityControlTest
IndirectELISA –todeterminetiteroftheantibody
SDSPAGE–todeterminepurityoftheantibody
Note
Thisproductisforresearchuseonly.