The ImmunoStar Substance P antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat substantia nigra and spinal cord using biotin/avidin-HRP techniques. Recommended primary dilution is 1/6000-1/8000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique. The specificity of the antiserum for Substance P was demonstrated using soluble pre-adsorption with the peptides in question at a final concentration of 10 µg of peptide per mL of diluted antiserum. Substance P immunolabeling was completely abolished by pre-adsorption with Substance P. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: neurokinin A, neurokinin B, somatostatin and neuropeptide K.

Photo Description: IHC image of rat spinal cord (top above) and rat tegmentum (below) staining for substance p. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning for brain and frozen sectioning for spinal cord. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.The immunofluorescent photo (second from the top) is wholemount mouse duodenum immunostained for Substance P (green, Alexa Fluor 488) and ANNA (neuron marker; red, Alexa Fluor 647).  Photo courtesy of Ryan Hamnett, PhD, Department of Neurosurgery Stanford University

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Bird, Bullfrog, Cat, Chick, Chicken, Dog, Dolphin, Doves, Equine, Fish, Frog, Gerbil, Goat, Goldfish, Guinea Pig, Hamster, Horse, Human, Monkey, Mouse, Mudpuppy, Narwhal, Parrot, Pig, Pig, Pigeon, Quail, Rabbit, Ram (Sheep), Rana Pipiens (Leopard Frog), Rat, Ratfish, Salmon, Scorpaena Porcus (Fish), Sea Lamprey, Sheep, Snail, Sparus Aurata (Fish), Squalus Acanthias (Dogfish), Squirrel, Starfish, Sting Ray, Sturgeon (Fish), Three-Spined Stickleback (Fish), Trout, Turtle, Vole, Zebra Finch, Zebrafish

Availability: In Stock

Alternate Names:  Protachykinin-1; PPT; tachykinin peptide hormone; NKA, NKNA, TAC2; tachykinin, precursor 1, anti-substance P

Gene Symbol:  TAC1RRID:  AB_572266Database Links:  Entrez Gene: 100227778    Bird  Entrez Gene: 101095481    Cat  Entrez Gene: 420573         Chick  Entrez Gene: 475239         Dog    Entrez Gene: 101331268    Dolphin  Entrez Gene: 102088620    Doves  Entrez Gene: 100052324    Equine  Entrez Gene: 779508         Frog  Entrez Gene: 102191397    Goat  Entrez Gene: 100713032    Guinea Pig  Entrez Gene: 100760914    Hamster  Entrez Gene: 6863             Human  Entrez Gene: 697350         Monkey  Entrez Gene: 21333           Mouse  Entrez Gene: 104412817    Parrot  Entrez Gene: 100525179    Pig  Entrez Gene: 107309132    Quail  Entrez Gene: 100009275    Rabbit  Entrez Gene: 100034669    Ram  Entrez Gene: 24806           Rat  Entrez Gene: 101972401    Squirrel  Entrez Gene: 102459998    Turtle  Entrez Gene: 101979426    Vole  Entrez Gene: 555634         Zebrafish