Product Specifications:
Item# 1103: Murine anti-p24 HIV-1 Monoclonal Antibody
Antibody Class: IgG
Concentration: See vial
Mass/vial: 100µg
Purity: >95%
Stabilizer: None
Preservative: None
Physical State: Liquid
Storage: -75°C
Stability: At least 12 months at -75°C
Application: ELISA,Western,ELISA, Immunoprecipitation,Immunocytochemistry, Immunohistochemistry.
Description: High affinity murine anti-p24 HIV-1 IIIB mAb.
Specificity: Reacts with native and recombinant HIV-1 p24 in Elisa and Western Elisa. Detects HIV-1 p24 in capture Elisa assays.
Biological Activity: Not determined.
Application and Instructions for use
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA may be performed at 10-100ng of mAb depending on the amount of immobilized antigen in the assays. Western ELISA requires 1-5µg of mAb.
SIMILAR PRODUCTS
Item#: 1103-P Peroxidase-Conjugated Murine anti-p24 HIV-1 IIIB
Application: ELISA, Western ELISA, Histocytochemistry, Histocytology.
Item#: 1103-F Fluorescene Murine anti-p24 HIV-1 IIIB
Application: Cell Staining
Item#: 1103-G Colloidal Gold-Conjugated Murine anti-p24 HIV-1 IIIB
Application: Dot Blots, EM.
Item#: 1103-M Magnetic Murine anti-p24 HIV-1 IIIB
Application: Immunoprecipitation.
Research paper related to Murine mAb p24 HIV-1 Clone 4F6
A highly sensitive and dynamic immunofluorescent cytometric bead assay for the detection of HIV-1 p24
MicroPlex carboxymethylated microspheres region 42 (5.6μm in diameter, catalog number L100-C142-01, Luminex Corporation, Austin, TX) were coupled to a high affinity anti-p24 monoclonal antibody (catalog # 1103-clone 4F6, ImmunoDiagnostics, Inc., Woburn, MA).
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手头上，有eBioscience家的同型抗体，为PE标记（mouseIgG1,k），说明书写使用为2.5ug/test。
按照说明书的浓度加入后结果发现，同型对照抗体组比实验组和阴性对照组（不表达CD80）荧光强度要高，很明确我的同型对照加入的剂量多了，但正确的加法是多少？按照质量算吗？请高手指教，非常感谢！！
比如你用anti-MHC-II，mouse IgG1-FITC，那同型就用非特异性的mouse IgG1-FITC，同样浓度
