AboutInVivoPlushumanIgG2isotypecontrol
ThehumanIgG2isotypecontrolantibodyispurifiedfromhumanmyelomaserumandisofunknownspecificity.Thisantibodyissuitableforuseasanon-targetingisotypecontrolinvariousinvitroandinvivostudies.ItcanalsobeusedasanegativecontrolinvariousdiagnosticapplicationssuchasELISA,Westernblot,immunofluorescence,immunohistochemistry,immunoprecipitation,andflowcytometry.Forresearchuseonly.
InVivoPlushumanIgG2isotypecontrolSpecifications
| Isotype | HumanIgG2,λ |
| RecommendedInVivoPureDilutionBuffer | InVivoPurepH7.0DilutionBuffer(IP0070) |
| Endotoxin |
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| Purity |
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| Aggregation |
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| Formulation |
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| Sterility | 0.2μMfiltered |
| Production | Purifiedfromhumanmyelomaserum |
| Purification | ProteinA |
| Storage | Theantibodysolutionshouldbestoredatthestockconcentrationat4°C.Donotfreeze. |
| HumanPathogenTestResults |
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| MurinePathogenTestResults |
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| MolecularWeight | 150kDa |
| RRID | AB_2715459 |
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英 ['aɪsətaɪp]美 ['aɪsəˌtaɪp]
n. 同型动物(或植物),图形文字,象征性图像;同位型;同模;同号模式
手头上,有eBioscience家的同型抗体,为PE标记(mouseIgG1,k),说明书写使用为2.5ug/test。
按照说明书的浓度加入后结果发现,同型对照抗体组比实验组和阴性对照组(不表达CD80)荧光强度要高,很明确我的同型对照加入的剂量多了,但正确的加法是多少?按照质量算吗?请高手指教,非常感谢!!
其同型为;percp-cy5.5(25ug包装)浓度:0.2mg/ml,
那么如果CD45RA抗体如果加入20ul,那同型抗体应该加入多少ml:
我自己的初步计算是:1ul的3分之1.不知道我算对了吗?应该怎么加才好呢?

