| Catalogue number | crb2115048e |
|---|---|
| Antibody | Anti-Phospho-GluR1 (pS845) antibody |
| Antigen Peptide | KLH conjugated synthetic peptide crb1200334e |
| Protein ID | UniProtKB - P35438 |
| Aliases | GluA1 |
| Cross-Reactivity | Human, rat, mouse, (predicted) goat |
| Host Species | Anti-Rabbit |
| Antibody Type | Polyclonal |
| Label | Labelled with Alexa Fluor® 488 under optimal conditions. Ex: 495nm Em:519nm |
| Concentration | 1mg/ml |
| Validation | ELISA |
| Target | phosphorylated GluR1 (pS845) |
| Disease Area | Neurodegenerative |
| Family | AMPA receptor |
| Specificity | Protein |
| Storage | Supplied in PBS containing 0.01% sodium azide. The product should be stored undiluted and protected from prolonged exposure to light. Short term storage between 2-8°C and long term storage below -20°C |
| References | Khatib, T., Whiting, A., Chisholm, D., Redfern, C., Müller, B. and McCaffery, P. (2019). A Bioluminescence Reporter Assay for Retinoic Acid Control of Translation of the GluR1 Subunit of the AMPA Glutamate Receptor. Mol Neurobiol. PMID: 30972628 Spaethling, J., Le, L. and Meaney, D. (2012). NMDA receptor mediated phosphorylation of GluR1 subunits contributes to the appearance of calcium-permeable AMPA receptors after mechanical stretch injury. Neurobiol Dis, 46(3), 646-654. PMID: 22426393 |
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例如 GST (1-109):
•epitope corresponding to amino acids 1-109 mapping at the N-terminus of GST of Schistosoma japonicum origin
•recommended for detection of GST fusion proteins and glutathione-S-transferase (GST) of Schistosoma japonicum origin by WB, IP, IF and ELISA
还有Schistosoma japonicum origin 跟我载体pGEX4T-1表达的融合蛋白可用Western Blot吗?它们有什么关系?
十分感谢
求助:为什么用蛋白抗体仅出来一条带,而用Flag抗体则出来两条带?
谢谢大家啊
图一:蛋白抗体
图二Flag抗体
百度上的,,,,,搬运
义翘神州的标签抗体(myc)采用的免疫原是A synthetic Myc tag peptide conjugated to KLH 。参看下图的验证结果。
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根据这个信息进行适宜的选择。
但是His标签是目前最普及最方便的融合标签,所以His标签的抗体,不管是单抗还是多抗,鼠源还是兔源都是商品化程度非常高的的抗体。这样的话因为抗体原因失败的可能性就非常小了。

