Human CD147 (EMMPRIN, Basigin) is a transmembrane glycoprotein expressed more intensely on thymocytes than on mature peripheral blood T cells. Expression of CD147 in bronchial epithelial cells has been correlated with incidence of COPD(3).
Isotype: Murine IgG1
Immunogen: Human T cell line Molt 13
Specificity: Antibody UM-8D6 recognizes extracellular epitope 2 within the n-terminal Ig domain (1) of the human CD147 molecule, about 35-40 kd (2)
Functional Applications: UM-8D6 inhibits homotypic aggregation, adhesion to matrix proteins and migation through matrigel (1). This antibody can inhibit homotypic induction of MMP (Matrix metalloproteinase), as well as cyclophilin mediated chemotactic resposes (4). It has been shown to inhibit proliferation of stimulated T cells in vitro (7). UM-8D6 is recommended for use in Western blot at 0.1 - 1 ug/ml (5). It works on acetone fixed frozen section IHC (6).
References:
1. Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 760-766.
2. A.H. Kirsch, D.A. Fox et al, (1997) Tissue Antigens 50:147-152.
3. Jouneau S, K.F. Chung, et al. (May 2011) Respirology 16(14): 705-712.
4. Yurchenko V, M Bukrinsky, et al. (2010) Clin Exp Immunol 160(3): 305-317.
5. O Najyb, L Brissette, E Rassart (June 2015) J Biol Chem 290: 16077-16087. PMID: 25918162
6. Y Noguchi, A Ito, et al (2003) J CLin Endocrinol Metab 88(12): 6063-72. PMID: 14671212
7. Smriti M Agrawal, V Wee Yong, et al. (2012) J Neuroinflammation 9: 64. doi: 10.1186/1742-2094-9-64
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抗体指机体的免疫系统在抗原刺激下,由B淋巴细胞分化成的浆细胞所产生的、可与相应抗原发生特异性结合反应的免疫球蛋白.
载体,你说的是那种载体?生命科学载体有2种,一种是分布于膜上的载体蛋白,和物质运输有关.另一种是基因工程里面的携带外源DNA的一段DNA片段,例如质粒、噬菌体和动植物病毒.
所以,抗体全部是蛋白质,载体部分是蛋白质.
义翘神州的标签抗体（myc）采用的免疫原是A synthetic Myc tag peptide conjugated to KLH 。参看下图的验证结果。
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免疫荧光是通过抗体上标记的荧光基团发光
望不吝赐教！
但是His标签是目前最普及最方便的融合标签，所以His标签的抗体，不管是单抗还是多抗，鼠源还是兔源都是商品化程度非常高的的抗体。这样的话因为抗体原因失败的可能性就非常小了。
