Human CD147 (EMMPRIN, Basigin) is a transmembrane glycoprotein expressed more intensely on thymocytes than on mature peripheral blood T cells. Expression of CD147 in bronchial epithelial cells has been correlated with incidence of COPD(3).
Isotype: Murine IgG1
Immunogen: Human T cell line Molt 13
Specificity: Antibody UM-8D6 recognizes extracellular epitope 2 within the n-terminal Ig domain (1) of the human CD147 molecule, about 35-40 kd (2)
Functional Applications: UM-8D6 inhibits homotypic aggregation, adhesion to matrix proteins and migation through matrigel (1). This antibody can inhibit homotypic induction of MMP (Matrix metalloproteinase), as well as cyclophilin mediated chemotactic resposes (4). It has been shown to inhibit proliferation of stimulated T cells in vitro (7). UM-8D6 is recommended for use in Western blot at 0.1 - 1 ug/ml (5). It works on acetone fixed frozen section IHC (6).
References:
1. Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 760-766.
2. A.H. Kirsch, D.A. Fox et al, (1997) Tissue Antigens 50:147-152.
3. Jouneau S, K.F. Chung, et al. (May 2011) Respirology 16(14): 705-712.
4. Yurchenko V, M Bukrinsky, et al. (2010) Clin Exp Immunol 160(3): 305-317.
5. O Najyb, L Brissette, E Rassart (June 2015) J Biol Chem 290: 16077-16087. PMID: 25918162
6. Y Noguchi, A Ito, et al (2003) J CLin Endocrinol Metab 88(12): 6063-72. PMID: 14671212
7. Smriti M Agrawal, V Wee Yong, et al. (2012) J Neuroinflammation 9: 64. doi: 10.1186/1742-2094-9-64
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想做个蛋白的定位，可是没有好抗体，就想做个转基因小鼠，在基因后面加一个标签。但是之前没有相关经验，不知道哪一种tag更好呢，更适合在小鼠里表达呢？因为我蛋白比较小，加GFP那么大的话可能会影响表达。比较小的tag例如HA，Flag还有His等，不知道哪个更适合一些？求大神们给些经验。
但是His标签是目前最普及最方便的融合标签，所以His标签的抗体，不管是单抗还是多抗，鼠源还是兔源都是商品化程度非常高的的抗体。这样的话因为抗体原因失败的可能性就非常小了。
百度上的，，，，，搬运
义翘神州的标签抗体（myc）采用的免疫原是A synthetic Myc tag peptide conjugated to KLH 。参看下图的验证结果。
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此酶溶于水和50%饱和度以下的硫酸铵溶液。
求助：为什么用蛋白抗体仅出来一条带，而用Flag抗体则出来两条带？
谢谢大家啊
图一：蛋白抗体
图二Flag抗体
例如 GST (1-109):
•epitope corresponding to amino acids 1-109 mapping at the N-terminus of GST of Schistosoma japonicum origin
•recommended for detection of GST fusion proteins and glutathione-S-transferase (GST) of Schistosoma japonicum origin by WB, IP, IF and ELISA
还有Schistosoma japonicum origin 跟我载体pGEX4T-1表达的融合蛋白可用Western Blot吗?它们有什么关系？
十分感谢
