Tissue-Tearor
The Tissue-Tearor is a rotor/stator type tissue homogenizer which can rapidly homogenize, disrupt, and emulsify plant and animal samples in 0.5 - 1000 ml of liquid. Complete homogenization of tissues (muscle, liver, breast tissue, etc.) is usually achieved in ten to thirty seconds. Little, if any, heat is produced during the process. Rotor/stator homogenizers cannot efficiently disrupt microorganisms. This "limitation" can be an advantage if you want to homogenize or disperse tissue sample (or soil or waste samples) in order to recover viable bacteria or viruses for plating and analysis.
If you need a 230 volt unit, please select models that end in EUR. When ordering select one of our four different probe sizes - 04, 07, XL, and 14. Unlike most competitive Brands of rotor-stator homogenizer, THE TISSUE-TEAROR COMES COMPLETE WITH BOTH THE HIGH-SPEED MOTOR AND ONE STAINLESS STEEL PROBE OF YOUR CHOICE. See chart below for probe selection guidance. Additional probes are available. All are interchangeable with the TT motor unit. A cord-less variety of the TissueTearor is also available with model numbers ending in CL.
Our Price : $0.00
The Tissue-Tearor comes complete with a high speed, variable speed electric motor, one probe of your choice and a plastic storage stand. All probes are interchangeable with the Tissue-Tearor motor. See Parts & Acc's to order additional probes alone. The Tissue-Tearor with the 14 mm diameter probe does not come with a plastic storage stand.
| Catalog Number | Practical Working Range (ml) | Probe Diameter (mm) | Probe Length (cm) |
| 985370-04 | 0.2-5.0 | 4.5 | 4.8 |
| 985370-07 | 0.5-50 | 7 | 8.3 |
| 985370-14 | 5.0-1000 | 14 | 13.2 |
| 985370-XL | 0.5-50 | 7 | 10.9 |
* Dimensions taken from stator.
| Scents | Apricot, Lemon & Mango |
| Color | test |
- Sample Volume Range: 0.2 to 1000 ml, depending on the probe selected
- Universal Motor: 110-120 volt, AC, 1.2 amp. Or, for some overseas applications, 230 volt, AC
- Probes: Type 316 stainless steel with fluorocarbon bushings
- Weight: Approximately 1/2 Kg
- Use for tissue cell disruption, emulsification and extreme mixing
- Practical answer for small samples of 10 mg to a few grams
- Easy to hold and light weight for one hand operation. Can also be stand mounted
- Inert to aggressive solutions. Stainless steel probe easily cleaned when processing multiple samples
- All probes are interchangeable and can be removed for autoclaving
- Extremely high speed 32,000 rpm motor with variable speed control
AWAY FROM ELECTRICAL LINES? Consider the model 780CL Cordless Tissue-Tearor. The purchase includes a cordless (i.e., battery operated), high speed motor, one probe of your choice, a rechargeable 9.6 volt Li battery pack and a plug-in battery charger. The maximum speed of the 780CL is slightly slower than the line-operated TissueTearor (25,000 vs 35,000 rpm). However, 25,000 rpm is quite adequate for homogenizing soft tissue like muscle, liver, or brain. The price for the 780CL Tissue-Tearor is the same as the line powered 985370 series. To order a Cordless Tissue-Tearor, combine the 780CL model number with the probe of your choice using the above table (example: 780CL-07).
OPERATING INSTRUCTIONS. See http://www.biospec.com/instructions/tissue_tearor/.
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BioHomogenizer
The BioHomogenizer is a rotor-stator type tissue homogenizer designed to rapidly homogenize, disperse, and emulsify biological samples in aqueous media from 1 to 1000 ml. The BioHomogenizer includes both the motor and probe.
Cat. No. 1281, BioHomogenizer for 5-200ml samples, complete with 1.4 cm probeCat. No. 1281EUR, 230 volt version of No. 1281Cat. No. 1285, BioHomogenizer for 100-2000ml samples, complete with 2.5 cm probeCat. No. 1285EUR, 230 volt version of No. 1285
Read more >Price: $830.00
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望不吝赐教！
但是His标签是目前最普及最方便的融合标签，所以His标签的抗体，不管是单抗还是多抗，鼠源还是兔源都是商品化程度非常高的的抗体。这样的话因为抗体原因失败的可能性就非常小了。
单抗标签选择的一抗范围比较小一些，多抗标签选择的一抗范围可能相对大一些。而商品化二抗基本没什么差别。不管是选择什么样的抗体，在Western blot检测方面都是一样的
所以做Western blot的单抗和多抗标签并没有什么不同
（2）6 X His tag 序列：CAT CAT CAC CAT CAC CAT
（3）GST标签序列：
1ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGC
301 AACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCAT
351 TTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATT
401 GGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAAT
451 TAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATG
501 TTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGC
551 GGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACT
601 TTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAA
651 ATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGT
701 AACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACA
751 TGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAA
801 CGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTA
851 TATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACC
901 ATCCTCCAAAATCGGATCTGGTTCCGCGTGGATCCCCGGAATTCCCGGGT
951 CGACTCGAGCGGCCGCATCGTGACTGA
百度上的，，，，，搬运
求助：为什么用蛋白抗体仅出来一条带，而用Flag抗体则出来两条带？
谢谢大家啊
图一：蛋白抗体
图二Flag抗体
Flag标签系统是已被公认的用于表达、纯化以及检测融合蛋白的表达系统，广泛应用于Westernblotting、免疫细胞组化、免疫共沉淀、流式细胞术、蛋白纯化以及蛋白相互作用、蛋白分离等多个研究领域。Flag标签是一个与重组蛋白相融合的由8个亲水氨基酸（Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys）组成的多肽片断。FLAG标签只有8个氨基酸组成，因此它不会占据其他表位与结合域，避免导致融合蛋白的功能、分泌性以及转运发生改变。由于它的亲水性特点，FLAG标签倾向于定位在融合蛋白表面，因此更易与抗体结合以及被肠激酶酶解。
由于Flag标签可位于蛋白质的C端或N端，这一系统已用于各种细胞类型，包括细菌、酵母和哺乳细胞。由于Flag标签系统的纯化条件是非变性的，因此可以纯化所有有活性的融合蛋白。EarthOx的Anti-FlagTagMonoclonalAntibody可以用于检测和Flag标签融合表达蛋白的表达、细胞内定位，以及纯化、定性或定量检测Flag融合表达蛋白等。比如在免疫荧光IF的实验里，同时配合EarthOx的Dylight549荧光二抗，使用该抗体可以准确的定位出Flag融合表达蛋白在细胞内的位置（如图红色显示部分）。另外，做融合表达分析前，通过免疫共沉淀技术获得Flag融合表达蛋白也是非常重要的一步，因此选择一个合适的、可用于免疫共沉淀（Immunoprecipitation，IP）Flag标签抗体也是很必要的。EarthOx的Anti-FlagTagMonoclonalAntibody不仅能应用于细胞内定位的IF实验，同样还能应用于IP实验。1:200甚至更高的IP实验稀释比率，也说明了该抗体本身的具有很高的效价，同时，因为是单克隆抗体，因此特异性也很高。高效价所对应的高稀释比率也意味着超高性价比。
