PTEN (Phospho-Ser380/Thr382/Thr383) AntibodyPDF
Catalog Number:
E011056-1Amount:
50μg/50μlForm of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 yearImmunogen:
The antiserum was produced against synthesized phosphopeptide derived from human PTEN around the phosphorylation site of serine 380 and threonine 382/383 (R-Y-SP-D-TP-TP-D-S).Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
PTEN (phospho-Ser380/Thr382/Thr383) antibody detects endogenous levels of PTEN only when phosphorylated at serine 380 and threonine 382/383.Reactivity:
Human, Mouse, RatApplications:
WB: 1:500~1:1000 IHC:1:50~100 IF:1:100~1:200Swiss-Prot No.:
P60484References:
Al-Khouri AM, et al. (2005) J Biol Chem. 280(42):35195-35202.Torres J, et al. (2001) J Biol Chem. 276(2): 993-998.Vazquez F, et al. (2000) Mol Cell Biol. 20(14): 5010-5018.
P-Peptide - +Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using PTEN (phospho-Ser380/Thr382/Thr383) antibody (E011056).

Western blot analysis of extract from HT29 cells untreated or treated with calyculin A, using PTEN (phospho-Ser380/Thr382/Thr383) antibody (E011056).
Price:
145$Research Area:
Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

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抗体指机体的免疫系统在抗原刺激下,由B淋巴细胞分化成的浆细胞所产生的、可与相应抗原发生特异性结合反应的免疫球蛋白.
载体,你说的是那种载体?生命科学载体有2种,一种是分布于膜上的载体蛋白,和物质运输有关.另一种是基因工程里面的携带外源DNA的一段DNA片段,例如质粒、噬菌体和动植物病毒.
所以,抗体全部是蛋白质,载体部分是蛋白质.
义翘神州的标签抗体(myc)采用的免疫原是A synthetic Myc tag peptide conjugated to KLH 。参看下图的验证结果。
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免疫荧光是通过抗体上标记的荧光基团发光
望不吝赐教!
但是His标签是目前最普及最方便的融合标签,所以His标签的抗体,不管是单抗还是多抗,鼠源还是兔源都是商品化程度非常高的的抗体。这样的话因为抗体原因失败的可能性就非常小了。

