
JAK1(Phospho-Tyr1022) AntibodyPDF
Catalog Number:
E011149-2Amount:
100μg/100μlForm of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 yearImmunogen:
The antiserum was produced against synthesized phosphopeptide derived from human JAK1 around the phosphorylation site of tyrosine 1022 (K-E-YP-Y-T).Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
JAK1 (phospho-Tyr1022) antibody detects endogenous levels of JAK1 only when phosphorylated at tyrosine 1022.Reactivity:
Human, Mouse, RatApplications:
WB: 1:500~1:1000 IHC: 1:50~1:100Swiss-Prot No.:
P23458References:
Zheng H, et al.(2005)Mol Cell Proteomics. 4(6):721-730.Wang R, et al.(2003) Arch Biochem Biophys. 410(1): 7-15.P-Peptide - +Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using JAK1 (phospho-Tyr1022) antibody (E011149).
Western blot analysis of extract from thyroid cancer cell line Bph cells, using JAK1 (phospho-Tyr1022) antibody (E011149).
Price:
225$Research Area:
Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
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(2)抗体如何选择?——根据目标分子的标签类型,检测的方法、抗体的适用范围、商家有没有提供自身质检结果等进行选择。
此酶溶于水和50%饱和度以下的硫酸铵溶液。
百度上的,,,,,搬运
根据这个信息进行适宜的选择。
但是His标签是目前最普及最方便的融合标签,所以His标签的抗体,不管是单抗还是多抗,鼠源还是兔源都是商品化程度非常高的的抗体。这样的话因为抗体原因失败的可能性就非常小了。
百度上的,,,,,搬运
有用过FLAG标签抗体的高手们来指点下,FLAG标签抗体那个公司的效果好啊,我准备买。
同时希望能提供宝贵意见。
谢谢各位阿
义翘神州的标签抗体(myc)采用的免疫原是A synthetic Myc tag peptide conjugated to KLH 。参看下图的验证结果。
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