Polyclonal Antibody to Interferon Gamma (IFNg)
IFN-G; IFG; IFI; INFr; IFN, Immune Interferon
- Product No.PAA049Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig TypeIgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- ImmunogenRPA049Ra01-Recombinant Interferon Gamma (IFNg)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species MoreHomo sapiens (Human)
- ApplicationsWB; IHC; ICC; IP.If the antibody is used in flow cytometry, please check FCM antibodies.
- DownloadInstruction Manual
- UOM20µl100µl200µl1ml10ml
- FOBUS$ 70 For more details, please contact local distributors!US$ 162 For more details, please contact local distributors!US$ 232 For more details, please contact local distributors!US$ 580 For more details, please contact local distributors!US$ 2320 For more details, please contact local distributors!
SPECIFITY of the Polyclonal Antibody to Interferon Gamma (IFNg)
The antibody isa rabbit polyclonal antibody raised against IFNg. It has been selected for its ability to recognize IFNg in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Interferon Gamma (IFNg)
Western blotting: 0.5-2ug/mlImmunocytochemistry in formalin fixed cells: 5-20ug/mlImmunohistochemistry in formalin fixed frozen section: 5-20ug/mlImmunohistochemistry in paraffin section: 5-20ug/mlEnzyme-linked Immunosorbent Assay: 0.05-2ug/mlOptimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Interferon Gamma (IFNg)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Interferon Gamma (IFNg)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized ServiceProtein A/G Purification ColumnStaining Solution for Cells and TissuePositive Controlfor AntibodyTissue/Sections Customized ServicePhosphorylated Antibody Customized ServiceWestern Blot (WB) Experiment ServiceImmunohistochemistry (IHC) Experiment ServiceImmunocytochemistry (ICC) Experiment ServiceFlow Cytometry (FCM) Experiment ServiceImmunoprecipitation (IP) Experiment ServiceImmunofluorescence (IF) Experiment ServiceBufferDAB Chromogen KitSABC KitLong-arm Biotin Labeling KitReal Time PCR Experimental Service
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| Catalog No. | Organism species: Rattus norvegicus (Rat) | Applications (RESEARCH USE ONLY!) |
| APA049Ra01 | Active Interferon Gamma (IFNg) | Cell culture; Activity Assays. |
| EPA049Ra61 | Eukaryotic Interferon Gamma (IFNg) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA049Ra02 | Recombinant Interferon Gamma (IFNg) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA049Ra61 | Active Interferon Gamma (IFNg) | Cell culture; Activity Assays. |
| RPA049Ra01 | Recombinant Interferon Gamma (IFNg) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA049Ra01 | Polyclonal Antibody to Interferon Gamma (IFNg) | WB; IHC; ICC; IP. |
| PAA049Ra02 | Polyclonal Antibody to Interferon Gamma (IFNg) | WB; IHC; ICC; IP. |
| PAA049Ra06 | Polyclonal Antibody to Interferon Gamma (IFNg) | WB; IHC; ICC; IP. |
| LAA049Ra81 | FITC-Linked Polyclonal Antibody to Interferon Gamma (IFNg) | WB; IHC; ICC; IF. |
| LAA049Ra71 | Biotin-Linked Polyclonal Antibody to Interferon Gamma (IFNg) | WB; IHC; ICC. |
| MAA049Ra21 | Monoclonal Antibody to Interferon Gamma (IFNg) | WB; IHC; ICC; IP. |
| MEA049Ra | Mini Samples ELISA Kit for Interferon Gamma (IFNg) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA049Ra | ELISA Kit for Interferon Gamma (IFNg) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA049Ra | CLIA Kit for Interferon Gamma (IFNg) | Chemiluminescent immunoassay for Antigen Detection. |
| KSA049Ra01 | ELISA Kit DIY Materials for Interferon Gamma (IFNg) | Main materials for "Do It (ELISA Kit) Yourself". |
| CGA049Ra01 | cDNA of Interferon Gamma (IFNg) | PCR; Protein Expression; etc. |
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Flag标签系统是已被公认的用于表达、纯化以及检测融合蛋白的表达系统,广泛应用于Westernblotting、免疫细胞组化、免疫共沉淀、流式细胞术、蛋白纯化以及蛋白相互作用、蛋白分离等多个研究领域。Flag标签是一个与重组蛋白相融合的由8个亲水氨基酸(Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys)组成的多肽片断。FLAG标签只有8个氨基酸组成,因此它不会占据其他表位与结合域,避免导致融合蛋白的功能、分泌性以及转运发生改变。由于它的亲水性特点,FLAG标签倾向于定位在融合蛋白表面,因此更易与抗体结合以及被肠激酶酶解。
由于Flag标签可位于蛋白质的C端或N端,这一系统已用于各种细胞类型,包括细菌、酵母和哺乳细胞。由于Flag标签系统的纯化条件是非变性的,因此可以纯化所有有活性的融合蛋白。EarthOx的Anti-FlagTagMonoclonalAntibody可以用于检测和Flag标签融合表达蛋白的表达、细胞内定位,以及纯化、定性或定量检测Flag融合表达蛋白等。比如在免疫荧光IF的实验里,同时配合EarthOx的Dylight549荧光二抗,使用该抗体可以准确的定位出Flag融合表达蛋白在细胞内的位置(如图红色显示部分)。另外,做融合表达分析前,通过免疫共沉淀技术获得Flag融合表达蛋白也是非常重要的一步,因此选择一个合适的、可用于免疫共沉淀(Immunoprecipitation,IP)Flag标签抗体也是很必要的。EarthOx的Anti-FlagTagMonoclonalAntibody不仅能应用于细胞内定位的IF实验,同样还能应用于IP实验。1:200甚至更高的IP实验稀释比率,也说明了该抗体本身的具有很高的效价,同时,因为是单克隆抗体,因此特异性也很高。高效价所对应的高稀释比率也意味着超高性价比。
选择Flag标签抗体,主要根据实验的需要。一个抗体检测后应用类型越多,使用中选择余地就越大。要综合考虑抗体来源,特异性,稳定性,效价等等因素
百度上的,,,,,搬运
有用过FLAG标签抗体的高手们来指点下,FLAG标签抗体那个公司的效果好啊,我准备买。
同时希望能提供宝贵意见。
谢谢各位阿
望不吝赐教!
想做个蛋白的定位,可是没有好抗体,就想做个转基因小鼠,在基因后面加一个标签。但是之前没有相关经验,不知道哪一种tag更好呢,更适合在小鼠里表达呢?因为我蛋白比较小,加GFP那么大的话可能会影响表达。比较小的tag例如HA,Flag还有His等,不知道哪个更适合一些?求大神们给些经验。
抗体指机体的免疫系统在抗原刺激下,由B淋巴细胞分化成的浆细胞所产生的、可与相应抗原发生特异性结合反应的免疫球蛋白.
载体,你说的是那种载体?生命科学载体有2种,一种是分布于膜上的载体蛋白,和物质运输有关.另一种是基因工程里面的携带外源DNA的一段DNA片段,例如质粒、噬菌体和动植物病毒.
所以,抗体全部是蛋白质,载体部分是蛋白质.


