The antibody has a proven strong biotin-streptavidin/HRP staining at a 1/2000 - 1/4000 dilution in rat brainstem, cerebellum and adrenal medulla. The above image is of DBH positive neurons in rat brainstem, vibratome sectioned, ABC / DAB detection.Using Western blot of purified DBH the antiserum detects a triplet at approximately 72-74 kD. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.
Photo Description: IHC image of neurons staining for DBH in the rat brainstem. The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:4000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Host: Rabbit
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Species Reactivity: Bird, Cat, Ferret, Finch, Guinea Pig, Hamster, Hatchetfish, Human, Ilyanassa Obsoleta (Sea Snail), Monkey, Mouse, Pig, Quail, Rat, Sparrow, Starling, Steer (Cattle), Turkey, Turtle, Zebra Finch
Availability: In Stock
Alternate Names: DBM; DOPO; Dopamine beta-monooxygenase, anti-DBH
RRID: AB_572229Immunogen: Bovine DBHGene Symbol: DBHDatabase Links: Entrez Gene: 101082957 Cat Entrez Gene: 101687995 Ferrett Entrez Gene: 102038113 Finch Entrez Gene: 100727873 Guuinea Pag Entrez Gene: 100761411 Hamster Entrez Gene: 1621 Human Entrez Gene: 574105 Monkey Entrez Gene: 13166 Mouse Entrez Gene: 733609 Pig Entrez Gene: 107321868 Quail Entrez Gene: 25699 Rat Entrez Gene: 102067155 Sparrow Entrez Gene: 106851290 Starling Entrez Gene: 280758 Steer (Cattle) Entrez Gene: 100544779 Turkey Entrez Gene: 102455557 Turtle Entrez Gene: 100224356 Zebra Finch (Bird)
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抗体指机体的免疫系统在抗原刺激下,由B淋巴细胞分化成的浆细胞所产生的、可与相应抗原发生特异性结合反应的免疫球蛋白.
载体,你说的是那种载体?生命科学载体有2种,一种是分布于膜上的载体蛋白,和物质运输有关.另一种是基因工程里面的携带外源DNA的一段DNA片段,例如质粒、噬菌体和动植物病毒.
所以,抗体全部是蛋白质,载体部分是蛋白质.
义翘神州的标签抗体（myc）采用的免疫原是A synthetic Myc tag peptide conjugated to KLH 。参看下图的验证结果。
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免疫荧光是通过抗体上标记的荧光基团发光
望不吝赐教！
但是His标签是目前最普及最方便的融合标签，所以His标签的抗体，不管是单抗还是多抗，鼠源还是兔源都是商品化程度非常高的的抗体。这样的话因为抗体原因失败的可能性就非常小了。
