Photo Description: IHC image of mu opioid receptor staining in the rat striatum. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:10000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Host: Rabbit
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: Cat, Chicken, Frog, Guinea Pig, Human, Monkey, Mouse, Rat, Starling, Tadpole
Availability: In Stock
Alternate Names: MOR-1; Mu-type opioid receptor; M-OR1; Mu opiate receptor; Opioid receptor B; MUOR1; M-OR-1; MORA; oprm; opioid receptor, mu 1, anti-MOR
Gene Symbol: Oprm1RRID: AB_572251
Database Links:
Entrez Gene: 101084452 Cat Entrez Gene: 421644 Chicken Entrez Gene: 100487937 Frog Entrez Gene: 100379267 Guinea Pig Entrez Gene: 4988 Human Entrez Gene: 574141 Monkey Entrez Gene: 18390 Mouse Entrez Gene: 25601 Rat Entrez Gene: 106859038 Starling
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
义翘神州的标签抗体（myc）采用的免疫原是A synthetic Myc tag peptide conjugated to KLH 。参看下图的验证结果。
向左转|向右转
想做个蛋白的定位，可是没有好抗体，就想做个转基因小鼠，在基因后面加一个标签。但是之前没有相关经验，不知道哪一种tag更好呢，更适合在小鼠里表达呢？因为我蛋白比较小，加GFP那么大的话可能会影响表达。比较小的tag例如HA，Flag还有His等，不知道哪个更适合一些？求大神们给些经验。
此酶溶于水和50%饱和度以下的硫酸铵溶液。
例如 GST (1-109):
•epitope corresponding to amino acids 1-109 mapping at the N-terminus of GST of Schistosoma japonicum origin
•recommended for detection of GST fusion proteins and glutathione-S-transferase (GST) of Schistosoma japonicum origin by WB, IP, IF and ELISA
还有Schistosoma japonicum origin 跟我载体pGEX4T-1表达的融合蛋白可用Western Blot吗?它们有什么关系？
十分感谢
