Product Specifications:
Item# 2001: Recombinant HIV-2 (ROD) Glycoprotein gp105
Concentration: See vial
Mass/vial: 100ug
Volume/vial: See vial
Diluent: PBS
Purity: >90%
Stabilizer: None
Preservative: None
Storage: -75°C
Physical State: Frozen Liquid
Stability: At least 12 months at -75°C.
Application: Human Diagnostics,Drug Screening,CD4 Binding,Vaccines,Immunization,T-Cell Activation.
Description: Full length Recombinant HIV-2 (ROD) gp105 glycoprotein produced in the Baculovirus Expression System.
Purification: This protein is purified by immunoaffinity chromatography to >90% purity as determined by SDS-PAGE, reduced.
Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity and HIV-2 human serum polyclonal antibodies in ELISA and Western ELISA.
Biological Activity: This protein binds to human T-cell receptor CD4, albeit with lower affinity compared to HIV-1 gp120, in ELISA and Western ELISA as determined by CD4/gp105/Anti- gp105 mAb-peroxidase capture ELISA. This protein activates human T-Lymphocytes (CD4+, CD4-), in vitro, as measured by RNA synthesis during G0 to G1 transition phase of antigen-binding competent cells.
Application and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 10-100ng of immobilized protein under standard ELISA conditions.
Item#: 2001-P Peroxidase-Conjugated rgp105 HIV-2 (ROD)
Application: Human diagnostics, CD4 binding, Drug screening, Human T-cell CD4 studies.
Item#: 2001-G Colloidal gold-Conjugated rgp105 HIV-2 (ROD)
Application: Human diagnostics, CD4 binding, Drug screening, FACS analysis.
Item#: 2001-F Flourescene-Conjugated rgp105 HIV-2 (ROD)
Application: FACS analysis, Histocytology.
Item#: 2001-M Magnetically-Coupled rgp105 HIV-2 (ROD)
Application: Human diagnostics, Human B and T-cell panning.
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此酶溶于水和50%饱和度以下的硫酸铵溶液。
（2）抗体如何选择？——根据目标分子的标签类型，检测的方法、抗体的适用范围、商家有没有提供自身质检结果等进行选择。
想做个蛋白的定位，可是没有好抗体，就想做个转基因小鼠，在基因后面加一个标签。但是之前没有相关经验，不知道哪一种tag更好呢，更适合在小鼠里表达呢？因为我蛋白比较小，加GFP那么大的话可能会影响表达。比较小的tag例如HA，Flag还有His等，不知道哪个更适合一些？求大神们给些经验。
单抗标签选择的一抗范围比较小一些，多抗标签选择的一抗范围可能相对大一些。而商品化二抗基本没什么差别。不管是选择什么样的抗体，在Western blot检测方面都是一样的
所以做Western blot的单抗和多抗标签并没有什么不同
求助：为什么用蛋白抗体仅出来一条带，而用Flag抗体则出来两条带？
谢谢大家啊
图一：蛋白抗体
图二Flag抗体
（2）6 X His tag 序列：CAT CAT CAC CAT CAC CAT
（3）GST标签序列：
1ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGC
301 AACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCAT
351 TTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATT
401 GGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAAT
451 TAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATG
501 TTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGC
551 GGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACT
601 TTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAA
651 ATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGT
701 AACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACA
751 TGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAA
801 CGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTA
851 TATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACC
901 ATCCTCCAAAATCGGATCTGGTTCCGCGTGGATCCCCGGAATTCCCGGGT
951 CGACTCGAGCGGCCGCATCGTGACTGA
