Product Specifications:
Item# 2001: Recombinant HIV-2 (ROD) Glycoprotein gp105
Concentration: See vial
Mass/vial: 100ug
Volume/vial: See vial
Diluent: PBS
Purity: >90%
Stabilizer: None
Preservative: None
Storage: -75°C
Physical State: Frozen Liquid
Stability: At least 12 months at -75°C.
Application: Human Diagnostics,Drug Screening,CD4 Binding,Vaccines,Immunization,T-Cell Activation.
Description: Full length Recombinant HIV-2 (ROD) gp105 glycoprotein produced in the Baculovirus Expression System.
Purification: This protein is purified by immunoaffinity chromatography to >90% purity as determined by SDS-PAGE, reduced.
Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity and HIV-2 human serum polyclonal antibodies in ELISA and Western ELISA.
Biological Activity: This protein binds to human T-cell receptor CD4, albeit with lower affinity compared to HIV-1 gp120, in ELISA and Western ELISA as determined by CD4/gp105/Anti- gp105 mAb-peroxidase capture ELISA. This protein activates human T-Lymphocytes (CD4+, CD4-), in vitro, as measured by RNA synthesis during G0 to G1 transition phase of antigen-binding competent cells.
Application and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 10-100ng of immobilized protein under standard ELISA conditions.
Item#: 2001-P Peroxidase-Conjugated rgp105 HIV-2 (ROD)
Application: Human diagnostics, CD4 binding, Drug screening, Human T-cell CD4 studies.
Item#: 2001-G Colloidal gold-Conjugated rgp105 HIV-2 (ROD)
Application: Human diagnostics, CD4 binding, Drug screening, FACS analysis.
Item#: 2001-F Flourescene-Conjugated rgp105 HIV-2 (ROD)
Application: FACS analysis, Histocytology.
Item#: 2001-M Magnetically-Coupled rgp105 HIV-2 (ROD)
Application: Human diagnostics, Human B and T-cell panning.
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选择Flag标签抗体，主要根据实验的需要。一个抗体检测后应用类型越多，使用中选择余地就越大。要综合考虑抗体来源，特异性，稳定性，效价等等因素
有用过FLAG标签抗体的高手们来指点下，FLAG标签抗体那个公司的效果好啊，我准备买。
同时希望能提供宝贵意见。
谢谢各位阿
求助：为什么用蛋白抗体仅出来一条带，而用Flag抗体则出来两条带？
谢谢大家啊
图一：蛋白抗体
图二Flag抗体
免疫荧光是通过抗体上标记的荧光基团发光
想做个蛋白的定位，可是没有好抗体，就想做个转基因小鼠，在基因后面加一个标签。但是之前没有相关经验，不知道哪一种tag更好呢，更适合在小鼠里表达呢？因为我蛋白比较小，加GFP那么大的话可能会影响表达。比较小的tag例如HA，Flag还有His等，不知道哪个更适合一些？求大神们给些经验。
根据这个信息进行适宜的选择。
但是His标签是目前最普及最方便的融合标签，所以His标签的抗体，不管是单抗还是多抗，鼠源还是兔源都是商品化程度非常高的的抗体。这样的话因为抗体原因失败的可能性就非常小了。
