
Product information | Key steps | References | Technical support | Reviews |
Product overview
Product name | Dopamine ELISA kit - Urine/Plasma - Fast-Track |
Description | In vitro diagnostics enzyme immunoassay (ELISA) for the rapid determination of Dopamine levels in plasma and urine samples. |
Labels | IVD, CE |
Format | 96-well plate |
Samples | 10 µl urine, 300 µl plasma |
Cross specie-reactivity | React with all species |
Standard range | 0 / 4.5 - 2 000 ng/ml |
Sensitivity | 4.5 ng/ml urine, 25 pg/ml plasma |
Specificity | No significant cross-reactivity was observed with Dopamine analogs such as Adrenaline, Noradrenaline, Metanephrine, Normetanephrine, Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid. |
Assay time | 4 hours |
Storage | Store at 2-8°C for up to 6 months. |
Datasheets | Instructions for use , Material safety datasheet |
Key steps
Sample collection & storage | Plasma: EDTA-Plasma samples only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 - 8°C; for longer periods (up to 6 months) at - 20°C. Urine: Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage: for longer periods (up to 6 months) at -20°C. |
Sample preparation | Sample preparation, extractiion and acylation (65min) |
ELISA | Dopamine antiserum incubation (2H), revelation and read steps (1h). |
Detailed protocol | Download instructions for use |
References
- Gaber EW & El-Attar HA. The relationship between dopamine and renalase in type 2 diabetic patients with and without diabetic nephropathy. British Journal of Diabetes & Vascular Disease May/June 2013 vol. 13 no. 3 130-137
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义翘神州的标签抗体(myc)采用的免疫原是A synthetic Myc tag peptide conjugated to KLH 。参看下图的验证结果。
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想做个蛋白的定位,可是没有好抗体,就想做个转基因小鼠,在基因后面加一个标签。但是之前没有相关经验,不知道哪一种tag更好呢,更适合在小鼠里表达呢?因为我蛋白比较小,加GFP那么大的话可能会影响表达。比较小的tag例如HA,Flag还有His等,不知道哪个更适合一些?求大神们给些经验。
此酶溶于水和50%饱和度以下的硫酸铵溶液。
例如 GST (1-109):
•epitope corresponding to amino acids 1-109 mapping at the N-terminus of GST of Schistosoma japonicum origin
•recommended for detection of GST fusion proteins and glutathione-S-transferase (GST) of Schistosoma japonicum origin by WB, IP, IF and ELISA
还有Schistosoma japonicum origin 跟我载体pGEX4T-1表达的融合蛋白可用Western Blot吗?它们有什么关系?
十分感谢

