LyophilizedPowderThisproductisfreezedried.Allwatermoleculeshavebeenremoved.
- Peptide(C)DDYGRPGIEKFREE,correspondingtoaminoacidresidues216-229ofhumanCaSR(Accession P41180). Extracellular,N-terminus.

CellsurfacedetectionofCaSRinliveintacthumanTHP-1monocyticleukemiacells:___ Cellsalone.
___ Cells+RabbitIgGisotypecontrol-FITC.
___ Cells+ Anti-CalciumSensingReceptor (extracellular)-FITCAntibody(#ACR-004-F),2.5µg.
CellsurfacedetectionofCaSRinliveintactmouseJ774macrophagecells:___ Cellsalone.
___ Cells+RabbitIgGisotypecontrol-FITC.
___ Cells+ Anti-CalciumSensingReceptor (extracellular)-FITCAntibody(#ACR-004-F),5µg.
- 1.Riccardi,D.andBrown,E.M.(2010)Am.J.Physiol.298,F485.
- 2.Hendy,G.N.etal.(2013)BestPract.Res.Clin.Endocrinol.Metab.27,285.
Thecalcium-sensingreceptor(CaSR)isaclassCG-protein-coupledreceptorencodedbyasinglecopygene.CaSRplaysamajorroleinthemaintenanceofaphysiologicalserumionizedCa2+ concentrationbyregulatingthecirculatinglevelsofparathyroidhormone1.
TheN-terminusofthereceptoriscomprisedofasignalpeptideof19 aminoacids.ThisterminusdirectsthenascentCaSRpolypeptideintothelumenoftheendoplasmicreticulum(ER)andisthencleaved.Theextracellulardomain(ECD)ofthematureproteinhasapproximately600 aminoacids,comprisedofabi-lobedVenus-flytrap-likedomain(VFT)connectedbyacysteine-richregionandashortpeptidelinkertotheseventransmembranedomain(TMD)withanintracellularC-terminaltailof216 aminoacids.CaSRfunctionsasahomodimerwithbothcovalentandnoncovalentinteractionsbetweentheVFTdomainsofeachmonomer. Ca2+ bindsinthecleftbetweenthetwolobesofeachVFTcausingthelobestocloseononeanotherandtheVFTtorotatewithtransferofaconformationalchangetothecysteine-richregion.
Intheparathyroidgland,up-regulationofCaSRby1,25-dihydroxyvitaminD[1,25(OH)2D]makestheglandmoreresponsivetoextracellularCa2+ andparathyroidhormone(PTH)secretionisreduced.Whilesomaticmutationsin CASR arerare,parathyroidglandsfrompatientswithprimaryorsevereuremicsecondaryhyperparathyroidismhavereducedCaSRexpression2.
Anti-CalciumSensingReceptor(extracellular)Antibody(#ACR-004)isahighlyspecificantibodydirectedagainstanepitopeofthehumanCaSR.Theantibodycanbeusedinwesternblot,immunohistochemistry,indirectflowcytometry,andimmunocytochemistryapplications.IthasbeendesignedtorecognizeCaSRfromhuman,rat,andmousesamples.
Anti-CalciumSensingReceptor(extracellular)-FITCAntibody(#ACR-004-F)isdirectlyconjugatedtofluoresceinisothiocyanate(FITC).Thislabeledantibodycanbeusedinimmunofluorescentapplicationssuchasdirectflowcytometryusinglivecells.
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例如 GST (1-109):
•epitope corresponding to amino acids 1-109 mapping at the N-terminus of GST of Schistosoma japonicum origin
•recommended for detection of GST fusion proteins and glutathione-S-transferase (GST) of Schistosoma japonicum origin by WB, IP, IF and ELISA
还有Schistosoma japonicum origin 跟我载体pGEX4T-1表达的融合蛋白可用Western Blot吗?它们有什么关系?
十分感谢
求助:为什么用蛋白抗体仅出来一条带,而用Flag抗体则出来两条带?
谢谢大家啊
图一:蛋白抗体
图二Flag抗体
百度上的,,,,,搬运
义翘神州的标签抗体(myc)采用的免疫原是A synthetic Myc tag peptide conjugated to KLH 。参看下图的验证结果。
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根据这个信息进行适宜的选择。
但是His标签是目前最普及最方便的融合标签,所以His标签的抗体,不管是单抗还是多抗,鼠源还是兔源都是商品化程度非常高的的抗体。这样的话因为抗体原因失败的可能性就非常小了。

