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Lyophilized Powder This product is freeze dried. All water molecules have been removed.
Antigen Incl. This antibody is shipped with its antigen FREE of charge!
- Peptide RTSDSRDHTRVDWKR(C), corresponding to amino acid residues 271-285 of rat GluR1 (Accession P19490). Extracellular, N-terminus.
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Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-GluR1 (GluA1) (extracellular) Antibody (#AGC-004), (1:200).
3,4. Anti-GluR1 (GluA1) (extracellular) Antibody, preincubated with the control peptide antigen.
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Expression of GluR1 in mouse hippocampusImmunohistochemical staining of mouse hippocampus with Anti-GluR1 (GluA1) (extracellular) Antibody (#AGC-004). GluR1 (green) is present in the stratum oriens (Or) and radiatum (Ra) but not in the pyramidal layer (P). Staining of the same section with mouse anti-parvalbumin (red) identifies the pyramidal layer.
- Rat hippocampal neurons (16 μg/ml) (Verpelli, C. et al. (2011) J. Biol. Chem. 286, 34839.).
- 1. Dingledine, R. et al. (1999) Pharmacol. Rev. 51, 7.
- 2. Sheng, M. and Lee, S.H. (2001) Cell 105, 825.
- 3. Song, I. and Huganir, R.L. (2002) Trends Neurosci. 25, 578.
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AMPA receptors are members of the glutamate receptor family of ion channels that also include the NMDA and Kainate receptors. The three subfamilies are named after the original synthetic agonists that were identified as selective ligands of each family.
The α-amino-3-hydroxy-5-methyl-4-isoazolepropionic acid (AMPA) receptor subfamily includes four members AMPA1-AMPA4 that are also known as GluR1-GluR4 respectively.
The functional AMPA channel is believed to be a tetramer, with most neuronal AMPA receptors being actually heterotetramers composed of AMPA1 plus AMPA2 or AMPA2 plus AMPA3, although homotetramers can also be found.
AMPA receptors are permeable to cations Na+, K+ and Ca2+. The Ca2+ permeability is dependent on the presence of AMPA2: whenever this subunit is present, the channel will be impermeable to Ca2+. The Ca2+ permeability of the AMPA2 subunit is determined by the presence of an arginine (R) at a critical site in the pore loop instead of a glutamine (Q) present in the same site in the other AMPA subunits. A post-transcriptional process known as RNA editing determines the presence of this R. Since most AMPA2 subunits in the adult brain have undergone RNA editing and most AMPA receptors contain the AMPA2 subunit, most native AMPA receptors will be impermeable to Ca2+.
Gating of AMPA receptors by glutamate is extremely fast and therefore the AMPA receptors mediate most excitatory (depolarizing) currents in the brain during basal neuronal activity. The depolarization caused by the activation of post-synaptic AMPA receptors is necessary for the activation of NMDA receptors that will open only in the presence of both glutamate and a depolarized membrane.
Synaptic strength, defined as the level of post-synaptic depolarization, can be long term (hence the term long term potentiation, LTP) and therefore induce changes in signaling and protein synthesis in the activated neuron. These changes are associated with memory formation and learning.
Changes in synaptic strength are thought to involve rapid movement of the AMPA receptors in and out of the synapses and a great deal of effort has focused in understanding the mechanisms that govern AMPA receptor trafficking.
Anti-GluR1 (GluA1) (extracellular) Antibody (#AGC-004) is a highly specific antibody directed against an extracellular epitope of the rat ionotropic glutamate receptor 1. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and live cell imaging applications. It has been designed to recognize GluR1 from human, mouse, and rat samples.
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望不吝赐教！
但是His标签是目前最普及最方便的融合标签，所以His标签的抗体，不管是单抗还是多抗，鼠源还是兔源都是商品化程度非常高的的抗体。这样的话因为抗体原因失败的可能性就非常小了。
单抗标签选择的一抗范围比较小一些，多抗标签选择的一抗范围可能相对大一些。而商品化二抗基本没什么差别。不管是选择什么样的抗体，在Western blot检测方面都是一样的
所以做Western blot的单抗和多抗标签并没有什么不同
（2）6 X His tag 序列：CAT CAT CAC CAT CAC CAT
（3）GST标签序列：
1ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGC
301 AACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCAT
351 TTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATT
401 GGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAAT
451 TAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATG
501 TTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGC
551 GGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACT
601 TTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAA
651 ATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGT
701 AACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACA
751 TGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAA
801 CGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTA
851 TATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACC
901 ATCCTCCAAAATCGGATCTGGTTCCGCGTGGATCCCCGGAATTCCCGGGT
951 CGACTCGAGCGGCCGCATCGTGACTGA
百度上的，，，，，搬运
求助：为什么用蛋白抗体仅出来一条带，而用Flag抗体则出来两条带？
谢谢大家啊
图一：蛋白抗体
图二Flag抗体
Flag标签系统是已被公认的用于表达、纯化以及检测融合蛋白的表达系统，广泛应用于Westernblotting、免疫细胞组化、免疫共沉淀、流式细胞术、蛋白纯化以及蛋白相互作用、蛋白分离等多个研究领域。Flag标签是一个与重组蛋白相融合的由8个亲水氨基酸（Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys）组成的多肽片断。FLAG标签只有8个氨基酸组成，因此它不会占据其他表位与结合域，避免导致融合蛋白的功能、分泌性以及转运发生改变。由于它的亲水性特点，FLAG标签倾向于定位在融合蛋白表面，因此更易与抗体结合以及被肠激酶酶解。
由于Flag标签可位于蛋白质的C端或N端，这一系统已用于各种细胞类型，包括细菌、酵母和哺乳细胞。由于Flag标签系统的纯化条件是非变性的，因此可以纯化所有有活性的融合蛋白。EarthOx的Anti-FlagTagMonoclonalAntibody可以用于检测和Flag标签融合表达蛋白的表达、细胞内定位，以及纯化、定性或定量检测Flag融合表达蛋白等。比如在免疫荧光IF的实验里，同时配合EarthOx的Dylight549荧光二抗，使用该抗体可以准确的定位出Flag融合表达蛋白在细胞内的位置（如图红色显示部分）。另外，做融合表达分析前，通过免疫共沉淀技术获得Flag融合表达蛋白也是非常重要的一步，因此选择一个合适的、可用于免疫共沉淀（Immunoprecipitation，IP）Flag标签抗体也是很必要的。EarthOx的Anti-FlagTagMonoclonalAntibody不仅能应用于细胞内定位的IF实验，同样还能应用于IP实验。1:200甚至更高的IP实验稀释比率，也说明了该抗体本身的具有很高的效价，同时，因为是单克隆抗体，因此特异性也很高。高效价所对应的高稀释比率也意味着超高性价比。
