LyophilizedPowderThisproductisfreezedried.Allwatermoleculeshavebeenremoved.
- Peptide(CD)TINGTWEGDELGYK,correspondingtoaminoacidresidues11-24ofratP2RY2(AccessionP41232).Extracellular,C-terminus.

CellsurfacedetectionofP2RY2inliveintactmouseJ774macrophagecells:___ Cells.
___ Cells+rabbitIgGisotypecontrol-FITC.
___ Cells+Anti-P2Y2Receptor(extracellular)-FITCAntibody(#APR-102-F),(2.5µg).
CellsurfacedetectionofP2RY2inliveintacthumanTHP-1monocyticleukemiacells:___ Cells.
___ Cells+rabbitIgGisotypecontrol-FITC.
___ Cells+Anti-P2Y2Receptor(extracellular)-FITCAntibody(#APR-102-F),(5µg).
- 1.Wang,L.etal.(2002)J.Cardiovas.Pharmacol.40,841.
- 2.Queiroz,G.etal.(2003)J.Pharmacol.Exp.Ther.307,809.
- 3.Ralevic,V.andBurnstock,G.(1998)Pharmacol.Rev.50,413.
- 4.Abbracchio,M.P.etal.(2003)TrendsPharmacol.Sci.24,52.
- 5.DiVirgilio,F.etal.(2001)Blood97,587.
- 6.Yerxa,B.R.etal.(2002)J.Pharmacol.Exp.Ther.302,871.
P2Yreceptors belongtotheG-proteincoupledreceptorsuperfamily.P2Yreceptorsmediatetheactionsoftheextracellularnucleotides(ATP,ADP,UTPandUDP)andregulateseveralphysiologicalresponses,amongthem,cardiacfunction,plateletaggregationandSMCproliferation1.
ThemammalianP2Yfamilycurrentlyincludeseightfunctionalreceptors; P2Y1,P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13,andtheUDP-glucosereceptor,nowrenamed P2Y142-4.
TheP2Y2receptorisabundantlyexpressedintheapicalmembranesofseveralepithelialtissuesincludingairway5.IthasbecomealeADIngtargetforthetreatmentofcysticfibrosis.ActivationofP2RY2increasesCl- effluxandmucociliaryclearance,effectsthatmightrelievesymptomsofcysticfibrosis.P2Y2agoNISTtherapyistestedinanattempttobypassthedysfunctioningCFTRchannel.SeveralP2Y2receptoragonists, especiallyINS37217,beingevaluatedaspossIBLetargetsforthetreatmentofcysticfibrosis6.
Anti-P2Y2Receptor(extracellular)Antibody (#APR-102)isahighlyspecificantibodydirectedagainstanepitopeoftheratprotein.Theantibodycanbeusedinwesternblot,indirectlivecellflowcytometry,andimmunohistochemistryapplications.IthasbeendesignedtorecognizeP2RY2fromrat,mouse,andhumansamples.
Anti-P2Y2Receptor(extracellular)–FITCAntibody (#APR-102-F)isdirectlyconjugatedtofluoresceinisothiocyanate(FITC).Thislabeledantibody canbeusedinimmunofluorescentapplicationssuchasdirectflowcytometryusinglivecells.
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例如 GST (1-109):
•epitope corresponding to amino acids 1-109 mapping at the N-terminus of GST of Schistosoma japonicum origin
•recommended for detection of GST fusion proteins and glutathione-S-transferase (GST) of Schistosoma japonicum origin by WB, IP, IF and ELISA
还有Schistosoma japonicum origin 跟我载体pGEX4T-1表达的融合蛋白可用Western Blot吗?它们有什么关系?
十分感谢
求助:为什么用蛋白抗体仅出来一条带,而用Flag抗体则出来两条带?
谢谢大家啊
图一:蛋白抗体
图二Flag抗体
百度上的,,,,,搬运
义翘神州的标签抗体(myc)采用的免疫原是A synthetic Myc tag peptide conjugated to KLH 。参看下图的验证结果。
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根据这个信息进行适宜的选择。
但是His标签是目前最普及最方便的融合标签,所以His标签的抗体,不管是单抗还是多抗,鼠源还是兔源都是商品化程度非常高的的抗体。这样的话因为抗体原因失败的可能性就非常小了。

