TB Green Premix Ex Taq (Tli RNase H Plus), an intercalating dye-based qPCR master mix for real-time RT-PCR (qPCR), is recommended for most real-time PCR applications including gene expression analyses, genotyping, and high-throughput studies. TB Green Premix Ex Taq (Tli RNase H Plus) includes TB Green, a qPCR reagent for intercalator-based RT-PCR (qPCR). In addition to being ideal for most standard real-time PCR reactions, this kit is recommended when a higher yield of PCR product is needed or when amplifying longer products (up to 570 bp). This TB Green qPCR premixis supplied at a 2X concentration and allows easy reaction assembly; bysimply adding primers, template, and sterile distilled water, intercalator-based qPCR can be performed.
TB Green Premix Ex Taq (Tli RNase H Plus), an intercalating dye-based qPCR master mix for real-time RT-PCR (qPCR), is recommended for most real-time PCR applications including gene expression analyses, genotyping, and high-throughput studies. TB Green Premix Ex Taq (Tli RNase H Plus) includes TB Green, a qPCR reagent for intercalator-based RT-PCR (qPCR). In addition to being ideal for most standard real-time PCR reactions, this kit is recommended when a higher yield of PCR product is needed or when amplifying longer products (up to 570 bp). This TB Green qPCR premixis supplied at a 2X concentration and allows easy reaction assembly; bysimply adding primers, template, and sterile distilled water, intercalator-based qPCR can be performed.
TB Green Premix Ex Taq(Tli RNase H Plus) contains Takara Ex Taq HS, a hot-start PCR enzyme that includes an anti-Taq antibody. The Takara Ex Taq HS enzyme, in combination with its qPCR optimized buffer, allows for high amplification efficiency and detection sensitivity in qPCR. The 2X TB Green qPCR premixalso contains Tli RNase H, a heat-resistant RNase H that minimizes qPCR inhibition as a consequence of residual mRNA in the input template cDNA. This product enables accurate detection of targets even during high-speed reactions, making it possible to conduct fast RT-PCR (qPCR) analyses and achieve excellent reproducibility and reliability.
ROX Reference Dyes are supplied as separate components with Cat. # RR420A, RR420B, RR420L, and RR420W. These reference dyes serve as convenient internal reference standards when normalizing non-PCR generated fluorescence fluctuations; such fluctuations often occur between different wells or over time in different instruments.
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（2）6 X His tag 序列：CAT CAT CAC CAT CAC CAT
（3）GST标签序列：
1ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGC
301 AACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCAT
351 TTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATT
401 GGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAAT
451 TAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATG
501 TTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGC
551 GGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACT
601 TTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAA
651 ATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGT
701 AACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACA
751 TGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAA
801 CGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTA
851 TATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACC
901 ATCCTCCAAAATCGGATCTGGTTCCGCGTGGATCCCCGGAATTCCCGGGT
951 CGACTCGAGCGGCCGCATCGTGACTGA
有用过FLAG标签抗体的高手们来指点下，FLAG标签抗体那个公司的效果好啊，我准备买。
同时希望能提供宝贵意见。
谢谢各位阿
百度上的，，，，，搬运
对于艾滋病和HIV感染的诊断，血清样品检测HIV抗体。可用真空采血管采血5—10ml（确定采样量）静脉血，室温下自然放置1—2h，待血液凝固后用3000rpm，离心15分钟，吸取血清HIV抗体检测。血清样品置2—8℃冰箱保存7天。
若用检测试剂检测HIV抗体，也可用末梢全血，成人、儿童或幼儿可选择中指、无名指采集、足跟部位采血。采血部位用75%酒精或碘伏认真消毒皮肤，用EDTA毛细管吸取血样。采血结束后，彻底止血，完成后的穿刺针头丢弃于耐穿透的容器内，妥善处理，严防职业暴露损伤。
采集血浆或全血应实验项目要求选用的抗凝剂，CD4/CD8+T淋巴细胞测定可选用EDTA或肝素。本实验室完成的实验尽早完成，完成检测项目，采集后在规定限时内及时送到，避开采集不当、暂存环境与时间的延误等因素而影响检测结果，如要检测CD4/CD8+T淋巴细胞测定的样品应选择合适的抗凝剂， 血样检测抗凝剂用EDTA（5 ml）若用EDTA钾盐血样品应在30h以内，尽早（8h以内）处理用ACD或肝素抗凝，血样在48h以内，尽早（8h）处理。静脉采集血样运用EDTA钾盐真空管，采集后立即颠倒混匀8--10次，使血液与抗凝剂混匀，对样品编号、日期、采集时间。
二、样品的保存：
用于抗体检测的血清或血浆样品，应存放在-20℃，短期在一周内检测的样品可存放于2-8℃。HIV检测阳性血清样品可保存至少十年。艾滋病筛查实验室存放阳性血清，阳性血清有资质的实验室可存放（确认艾滋病实验室）用于CD4/CD8+T淋巴细胞测定样品采集时
间超过48小时则检测。HIV检测阳性反应血清样品均保有着艾滋病检测确认实验室，，在HIV阳性反应血清样品较多的地区，艾滋病检测确认实验室应配置-20℃的低温冰箱和-80℃的超低温冰箱保存。
三、样品的运送：
确认阳性反应的血清或血浆，应WHO的三级包装系统。层容器：装样品、防渗漏。样品应置于带盖的试管内，试管上有明确的标记、受检者的姓名、种类和采集时间。在试管周围应垫有缓冲吸水，以防碰碎。随样品应附有送检单。要填写送检单种类信息，分开放置。层容器：要求耐受性好的、防渗漏、容纳保护层容器，容器便于消毒处理。层容器：是运输用于外层包装内，应易于消毒。在层容器外面要贴有标签（注明数量、收样单位等内容）血清或血浆样品应在2--8℃条件下专人运送。
检测检测CD4/CD8+T淋巴细胞测定的清或血浆温样品应在室温下（18--23℃）运送。但三级包装要求将盛样品的试管放好，避开使用玻璃容器，以保证不会破碎和渗漏。运送要有感染防护措施。
四、样品的接收
在有感染源设备的实验室内由经过培训、则在生物安全柜中戴手套、口罩、戴防护衣、带防护眼镜、的工作人员打开，用后的包裹应消毒。认真核对样品盒送检单、检查样品有无破损和渗漏，如渗漏立即将存留的样品移出，对样品管和渗漏消毒，检查样品情况，有无溶血、微生物污染、血脂过高及黄疸等情况，污染或溶血等情况样品被接受，则应将样品安全废弃，并要将样品情况立即通知送样人或单位。打开标本容器时要小心，以防皮肤或粘膜污染，以防内容物泼溅。
aware天猫唾液检测不用抽血！
