The Blyscan™ Glycosaminoglycan Assay is a quantitative dye-binding method for the analysis of sulfated proteoglycans and glycosaminoglycans, (sGAG).
Test material includes:
Cell culture derived material
Soluble sGAGs from fluids, such as synovial fluid, urine.
Cartilage
Connective tissue / tumours
Soluble sGAGs may be assayed directly, solid materials will require a preliminary papain extraction.
The assay can be used to measure the total sGAG content and can also be adopted to determine the O- and N-sulfated glycosaminoglycan ratio within test samples.
The dye label used in the assay is 1,9-dimethylmethylene blue and the dye is employed under conditions that provide a specific label for the sulfated polysaccharide component of proteoglycans or the protein free sulfated glycosaminoglycan chains.
Please note:
The assay is not suitable for small sulfated disaccharide fragments or for the samples containing alginates, as these contain uronic acid.
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