《微生物学》期末考试复习资料小抄版(考试必备)
Antibodygeneration:
Producedfromseraofgoatspre-immunizedwithhighlypure(98%)recombinantmurineIL-21.Anti-murineIL-21specificantibodywaspurifiedbyaffinitychromatographyemployingimmobilizedmurineIL-21matrix.Antigen:
recombinantmouseIL-21Stability:
Thelyophilizedantibodyisstableforatleast2yearsfromdateofreceiptat-20°C.Thereconstitutedantibodyisstableforatleasttwoweeksat2-8°C.Frozenaliquotsarestableforatleast6monthswhenstoredat-20°C.Reconstitution:
Reconstituteinsterilewatertoaconcentrationof0.1-1.0mg/mL.Description:
IL-21isproducedbyCD4+Tcellsinresponsetoantigenicstimulation.Itsactionenhancesantigen-specificresponsesofimmunecells.ThebiologicaleffectsofIL-21includeinductionofdifferentiationofT-cells-stimulatedB-cellsintoplasmacellsandmemoryB-cells,stimulation(inconjuction)withIL-4ofIgGproduction,andinductionofapoptoticeffectsinnaiveB-cellsandstimulatedB-cellsintheabsenceofT-cellsignaling.Additionally,IL-21promotestheanti-tumoractivityofCD8+T-cellsandNKcells.IL-21exertsitseffectthroughbindingtoaspecifictypeIcytokinereceptor,IL-21R,whichalsocontainsthegammachain(°C)foundinothercytokinereceptorsincludingIL-2,IL-4,IL-7,IL-9andIL-15.TheIL-21/IL-21RinteractiontriggersacascadeofeventswhichincludesactivationofthetyrosinekinasesJAK1andJAK3,followedbyactivationofthetranscriptionfactorsSTAT1andSTAT3.免责声明 本文仅代表作者个人观点,与本网无关。其创作性以及文中陈述文字和内容未经本站证实,对本文以及其中全部或者部分内容、文字的真实性、完整性、及时性本站不做任何保证或承诺,请读者仅作参考,并请自行核实相关内容。
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发布于 : 2018-06-23
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