Tissue-Tearor
The Tissue-Tearor is a rotor/stator type tissue homogenizer which can rapidly homogenize, disrupt, and emulsify plant and animal samples in 0.5 - 1000 ml of liquid. Complete homogenization of tissues (muscle, liver, breast tissue, etc.) is usually achieved in ten to thirty seconds. Little, if any, heat is produced during the process. Rotor/stator homogenizers cannot efficiently disrupt microorganisms. This "limitation" can be an advantage if you want to homogenize or disperse tissue sample (or soil or waste samples) in order to recover viable bacteria or viruses for plating and analysis.
If you need a 230 volt unit, please select models that end in EUR. When ordering select one of our four different probe sizes - 04, 07, XL, and 14. Unlike most competitive Brands of rotor-stator homogenizer, THE TISSUE-TEAROR COMES COMPLETE WITH BOTH THE HIGH-SPEED MOTOR AND ONE STAINLESS STEEL PROBE OF YOUR CHOICE. See chart below for probe selection guidance. Additional probes are available. All are interchangeable with the TT motor unit. A cord-less variety of the TissueTearor is also available with model numbers ending in CL.
Our Price : $0.00
The Tissue-Tearor comes complete with a high speed, variable speed electric motor, one probe of your choice and a plastic storage stand. All probes are interchangeable with the Tissue-Tearor motor. See Parts & Acc's to order additional probes alone. The Tissue-Tearor with the 14 mm diameter probe does not come with a plastic storage stand.
| Catalog Number | Practical Working Range (ml) | Probe Diameter (mm) | Probe Length (cm) |
| 985370-04 | 0.2-5.0 | 4.5 | 4.8 |
| 985370-07 | 0.5-50 | 7 | 8.3 |
| 985370-14 | 5.0-1000 | 14 | 13.2 |
| 985370-XL | 0.5-50 | 7 | 10.9 |
* Dimensions taken from stator.
| Scents | Apricot, Lemon & Mango |
| Color | test |
- Sample Volume Range: 0.2 to 1000 ml, depending on the probe selected
- Universal Motor: 110-120 volt, AC, 1.2 amp. Or, for some overseas applications, 230 volt, AC
- Probes: Type 316 stainless steel with fluorocarbon bushings
- Weight: Approximately 1/2 Kg
- Use for tissue cell disruption, emulsification and extreme mixing
- Practical answer for small samples of 10 mg to a few grams
- Easy to hold and light weight for one hand operation. Can also be stand mounted
- Inert to aggressive solutions. Stainless steel probe easily cleaned when processing multiple samples
- All probes are interchangeable and can be removed for autoclaving
- Extremely high speed 32,000 rpm motor with variable speed control
AWAY FROM ELECTRICAL LINES? Consider the model 780CL Cordless Tissue-Tearor. The purchase includes a cordless (i.e., battery operated), high speed motor, one probe of your choice, a rechargeable 9.6 volt Li battery pack and a plug-in battery charger. The maximum speed of the 780CL is slightly slower than the line-operated TissueTearor (25,000 vs 35,000 rpm). However, 25,000 rpm is quite adequate for homogenizing soft tissue like muscle, liver, or brain. The price for the 780CL Tissue-Tearor is the same as the line powered 985370 series. To order a Cordless Tissue-Tearor, combine the 780CL model number with the probe of your choice using the above table (example: 780CL-07).
OPERATING INSTRUCTIONS. See http://www.biospec.com/instructions/tissue_tearor/.
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BioHomogenizer
The BioHomogenizer is a rotor-stator type tissue homogenizer designed to rapidly homogenize, disperse, and emulsify biological samples in aqueous media from 1 to 1000 ml. The BioHomogenizer includes both the motor and probe.
Cat. No. 1281, BioHomogenizer for 5-200ml samples, complete with 1.4 cm probeCat. No. 1281EUR, 230 volt version of No. 1281Cat. No. 1285, BioHomogenizer for 100-2000ml samples, complete with 2.5 cm probeCat. No. 1285EUR, 230 volt version of No. 1285
Read more >Price: $830.00
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链接很容易，就用EDC或者CMC等碳二亚胺，是最容易在羧基和氨基之间催化形成酰胺键，反应条件就是0到4度，过夜，用磁力搅拌子温和搅拌，pH<7反应快，不过pH=7或稍微pH>7也关系不大。EDC或者CMC等碳二亚胺催化活性极高，而且一般的蛋白质的分子表面总有Lys,Arg,Asn,Gln，所以游离在分子表面的羧基和氨基总是不少的，参与反应的两种蛋白质用大致10：1的比例混合（哪种蛋白质便宜，哪种蛋白质的物质的量就大些，但是这不是全部原因），反应样品浓度就按照平时使用时的浓度或略微更低浓度（别用储存液的浓度）即可。很容易反应，而且EDC或者CMC等碳二亚胺是零臂连接试剂，用于空间位阻，形成多分子交联可能较小，即使形成了也很容易用分子筛层析按照分子量区分开。
参与反应的两种蛋白质用大致10：1的比例混合（哪种蛋白质便宜，哪种蛋白质的物质的量就大些，但是这不是全部原因），为了避免两种蛋白质之间发生交联成为串联，因为一种蛋白质形成串联的可能性低一些；即使要形成串联的蛋白质串珠，最好也不要是价格高的那种；另外，EDC或者CMC等碳二亚胺催化活性极高，室温下即会催化，所以要在0到4度，过夜，用磁力搅拌子温和搅拌，一方面避免碳二亚胺催化活性太高形成串联的蛋白质串珠，另一方面，保护蛋白质不变性；反应样品浓度就按照平时使用时的浓度或略微更低浓度（别用储存液的浓度）即可，为什么？因为浓度高了更易形成串联的蛋白质串珠结构，浓度低了，没有反应成功，通过分子筛层析还可以分离出来接着连接，如果形成了酰胺键再要专一性切开就没有那么容易了。
河南省郑州市所以食品添加剂有限公司 维生素H1价格 [未实名认证] 国产 1*20 435 元/kg
1） 物素N—羟基丁二酰亚胺酯（biotinyl-N-hydroxy-succinnimideBNHS）制备：
取物素1g混悬于12 mlN,N-二甲基甲酰胺(DMF)加0．6g N-羟基丁二酰亚胺(HOSU)0. 8 g二环基碳二亚胺(DCC)置于密闭容器内室温磁力搅拌作用夜滤滤液经旋转蒸干加10mL乙醚洗涤继加200 ml异丙醇使其重结晶获白色粉末状晶体
BNHS酯键-C=0基团与蛋白质赖氨酸氨基结合使物素标记蛋白含赖氨酸残基越或蛋白质等电点pI 6.0其标记效越BNHS适用于标记抗体及性偏碱性抗原
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