
Product Name | SensoLyte ® 520 MMP - 9 Assay Kit *Fluorimetric* |
Size | 1 kit |
Catalog # | AS-71155 |
US$ | $480 |
MMP-9 (gelatinase-B, collagenase-IV) is involved in quite a few diseases such as a cancer, angiogenesis, alopecia and metastasis. The SensoLyte® 520 MMP-9 Assay Kit uses a 5-FAM (fluorophore) and QXL520 (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities.In an intact FRETpeptide, the fluorescence of 5-FAM is quenched by QXL520. Upon the cleavage of the FRET peptide by MMP-9, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520 based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format.Members of the MMP family have poor substrate sequence specificity, making it difficult to use a peptide substrate alone to differentiate the activity of a particular MMP from other MMPs. If several MMPs are coexisting in your samples and you would like to specifically measure MMP-9 activity, please choose the SensoLyte® Plus MMP-9 Assay Kit, Cat# 72017. Kit size:100 assays | |
Detailed Information | ![]() ![]() |
Storage | -20°C |
Product Citations | Shin SJ, et al. (2020) Role of PAR1-Egr1 in the initiation of thoracic aortic aneurysm in Fbln4-deficient mice. Arteriosclerosis, Thrombosis, and Vascular Biology. 40(8):1905-17. doi: 10.1161/ATVBAHA.120.314560.Kalafatovic D, et al. (2016). MMP-9 triggered self-assembly of doxorubicin nanofiber depots halts tumor growth. Biomaterials, 98, 192-202.https://doi.org/10.1016/j.biomaterials.2016.04.039Pifer, MA. et al. (2014). Matrix metalloproteinase content and activity in low-platelet, low-leukocyte and high-platelet, high-leukocyte platelet rich plasma (PRP) and the biologic response to PRP by human ligament fibroblasts. Am J Sports Med 42, 1122. doi: 10.1177/0363546514524710.Spolidoro, M. et al. (2013). Inhibition of matrix metalloproteinases prevents the potentiation of nondeprived-eye responses after monocular deprivation in juvenile rats. Cereb Cortex 22, 725. doi: 10.1093/cercor/bhr158.Inoue, H. et al. (2012). Association of the MMP9 gene with childhood cedar pollen sensitization and pollinosis. J Hum Genet., 57, 176.Wigner, NA. et al. (2011). Urine matrix metalloproteinases (MMPSs) as biomarkers for the progression of fracture healing. Injury 10.1016/j.injury.2011.05.038.Koyama, Y. et al. (2010). Intracerebroventricular administration of an endothelin ETB-receptor agonist increases expression of matrix metalloproteinase-2 and -9 in rat brain. J Pharmacol Sci 114, 433.Yuan, H. et al. (2010). A novel pharmacophore model for the design of anthrax lethal factor inhibitors. Chem Biol Drug Design 76, 263.Allington, T. et al. (2009). Activated Abl kinase inhibits oncogenic transforming growth factor-β signaling and tumorigenesis in mammary tumors. FASEB J, 23, 4231-4243.Johnson, SL. et al. (2008). Rhodamine derivatives as selective protease inhibitors against bacterial toxins. Chem Biol Drug Des 71, 131.Soehnlein, O. et al. (2008). Neutrophil degranulation mediates severe lung damage triggered by streptococcal M1 protein. ERJ 32, 405.Johnson, SL. et al. (2007). A high-throughput screening approach to anthrax lethal factor inhibition. Bioorg Chem 35, 306. |
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河南省郑州市所以食品添加剂有限公司 维生素H1价格 [未实名认证] 国产 1*20 435 元/kg
链接很容易,就用EDC或者CMC等碳二亚胺,是最容易在羧基和氨基之间催化形成酰胺键,反应条件就是0到4度,过夜,用磁力搅拌子温和搅拌,pH<7反应快,不过pH=7或稍微pH>7也关系不大。EDC或者CMC等碳二亚胺催化活性极高,而且一般的蛋白质的分子表面总有Lys,Arg,Asn,Gln,所以游离在分子表面的羧基和氨基总是不少的,参与反应的两种蛋白质用大致10:1的比例混合(哪种蛋白质便宜,哪种蛋白质的物质的量就大些,但是这不是全部原因),反应样品浓度就按照平时使用时的浓度或略微更低浓度(别用储存液的浓度)即可。很容易反应,而且EDC或者CMC等碳二亚胺是零臂连接试剂,用于空间位阻,形成多分子交联可能较小,即使形成了也很容易用分子筛层析按照分子量区分开。
参与反应的两种蛋白质用大致10:1的比例混合(哪种蛋白质便宜,哪种蛋白质的物质的量就大些,但是这不是全部原因),为了避免两种蛋白质之间发生交联成为串联,因为一种蛋白质形成串联的可能性低一些;即使要形成串联的蛋白质串珠,最好也不要是价格高的那种;另外,EDC或者CMC等碳二亚胺催化活性极高,室温下即会催化,所以要在0到4度,过夜,用磁力搅拌子温和搅拌,一方面避免碳二亚胺催化活性太高形成串联的蛋白质串珠,另一方面,保护蛋白质不变性;反应样品浓度就按照平时使用时的浓度或略微更低浓度(别用储存液的浓度)即可,为什么?因为浓度高了更易形成串联的蛋白质串珠结构,浓度低了,没有反应成功,通过分子筛层析还可以分离出来接着连接,如果形成了酰胺键再要专一性切开就没有那么容易了。
叶酸和生物素都属于B族维生素,
对维持机体的生理功能有重要作用。

