Lyophilized Powder This product is freeze dried. All water molecules have been removed.
Antigen Incl. This antibody is shipped with its antigen FREE of charge!
- Peptide CRDPDYGTLIQE, corresponding to amino acid residues 27-38 of rat RAMP1 (Accession Q9JJ74). Extracellular, N-terminus.
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Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat pancreas (lanes 3 and 6):1-3. Anti-RAMP1 (extracellular) Antibody (#ARR-021).
4-6. Anti-RAMP1 (extracellular) Antibody, preincubated with the negative control antigen.
The antibody was used at a concentration of 1:400 for brain lysates and 1:200 for pancreas lysates.
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Expression of RAMP1 in rat PC12 cellsCell surface detection of RAMP1 in intact living rat pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-RAMP1 (extracellular) Antibody (#ARR-021), (1:50), (red). B. Live view of cells.
- 1. Kadmiel, M. et al. (2012) Adv. Exp. Med. Biol. 2012, 744, 49.
- 2. Jacob, A. et al. (2012) Adv. Exp. Med. Biol. 744, 87.
- 3. Conner, A.C. et al. (2004) Biochem. Soc. Trans. 32, 843.
- 4. Li, M. et al. (2014) PLoS One 9, e102356.
- 5. Shi, B. et al. (2014) Exp. Biol. Med. 239, 356.
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Receptor activity modifying protein-1 (RAMP1) is part of a three-member group (that further includes RAMP2 and RAMP3 – each encoded by a different gene) of transmembrane proteins that interact with G-protein coupled receptors (GPCRs) such as the calcitonin receptor-like receptors (CLRs)1. This interaction leads to the formation of the receptor for calcitonin gene related peptide (CGRP) and is necessary for the binding of the peptide to the receptor. The binding of RAMP2 and RAMP3 to CLR, on the other hand, creates the receptor for adrenomedullin, a peptide functionally and structurally related to calcitonin1,2.
RAMP1 (as well as the two other RAMPs) has a short intracellular domain and a single transmembrane domain. Its extracellular region contains four conserved cysteine residues that putatively form two disulfide bonds. This domain confers specificity to the receptor complex, whereas the transmembrane chain allows the association of the RAMPs with the GPCRs. In contrast to the other RAMPs, RAMP1 lacks glycosylation sites and therefore is unable to migrate to the cell surface independently. To reach the cell surface, it aggregates inside the cytoplasm and forms heterodimers with calcitonin3.
RAMP1 is highly prominent in the neuronal tissues (especially the trigeminal ganglion, striatum, cortex, olfactory bulb, dorsal root ganglion, and the spinal cord) but abundant as well in the electrical conducting system of the heart, blood vessels, and related sites1,2,4.
While not dramatically affected, RAMP1-KO mice are reported to have higher blood pressure and to suffer from repressed wound healing process, while overexpression of RAMP1 in animals leads to extravasation and vasodilation2. Interestingly, asthmatic symptoms in mice model of asthma were mitigated in mice that were RAMP1-deficient4. Furthermore mesenchymal stem cells overexpressing RAMP1 were shown to improve cardiac function and to reduce occurrences of over-thickened blood vessels5.
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat RAMP1. Anti–RAMP1 (extracellular) Antibody (#ARR-021) can be used in western blot and live cell imaging applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize RAMP1 from rat and mouse samples. The antibody does not recognize RAMP1 from human samples.
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这三样药是能混合在一起吃？
谢谢。
深夜，保姆觉得无聊就想去楼下看电视。但是她看不了，因为楼下没有电视（因为孩子的父母不希望他们的孩子看太多垃圾）。她就打电话给孩子的父母，问是否可以在他们的卧室看电视，当然孩子的父母同意了。
但保姆又想要最后一个请求。
她问是否可以用毯子或者衣服盖住那小丑雕像，因为那使她感到很害怕。
电话沉默了一会。
（此时爸爸在和保姆通话）
他说：带孩子离开房间……
我们将会叫警察……我们从来没有什么小丑雕像。
那小丑很可能是一个从监狱逃出来的杀人犯。
电话里沉默了一会儿。
（正在跟保姆通话的孩子的父亲）说：带上孩子们，离开房子……我们会通知警察……我们没有一个小丑雕像……
孩子们和保姆被小丑谋杀了。
结果是，小丑是一个从监狱里逃出来的杀人犯。
如果你不在5分钟内转发这个贴子，这个小丑在凌晨3点时将会拿着刀站在你的床前。
我在这里发了，这就是恶魔般的小丑没有杀我的原因
