To generate large amounts of a recombinant protein, many investigators have turned to baculovirus expression systems. Compared to bacterial expression systems, the posttranslational processing and folding of recombinant proteins produced in insect cells more closely resembles mammalian processes, and the yields of functional protein are often much greater.
To generate large amounts of a recombinant protein, many investigators have turned to baculovirus expression systems. Compared to bacterial expression systems, the posttranslational processing and folding of recombinant proteins produced in insect cells more closely resembles mammalian processes, and the yields of functional protein are often much greater.
BacPAK method
The BacPAK System uses the baculovirus Autographa californica nuclear polyhedrosis virus (AcMNPV) to produce target proteins in insect cells. The target gene is inserted into a shuttle vector, which is cotransfected into insect host cells with the linearized BacPAK6 Viral DNA. The specially designed BacPAK6 Viral DNA forces recombination between the virus and transfer vector, resulting in high recombination efficiency. Following recombination, a few viral plaques are picked and purified, and the recombinant phenotype is verified. The newly isolated recombinant virus can then be amplified and used to infect insect cell cultures to produce large amounts of the desired protein.If you wish, you can use pBacPAK8-GUS (sold as part of the BacPAK Baculovirus Expression System) as a positive control for the cotransfection step. This transfer vector has the E. coli beta-glucuronidase (GUS) gene cloned downstream of its polyhedrin promoter. Recombination of pBacPAK8-GUS with the BacPAK6 DNA digest generates recombinant viruses that express beta-glucuronidase. Expression of GUS can be detected by generation of a blue color from the chromogenic GUS substrate X-GLUC.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
苯妥英钠：控制室性心律失常和抑制房室传导
阿托品：用于心动过缓或房室阻滞
地高辛抗体Fab片断：
适用于危及生命的致死性中毒。
即可以用碱性磷酸酶标记的地高辛标抗体和 地高辛记的反应
也可以用辣根过氧化物酶标记的地高辛抗体和 地高辛记的反应
