Human Netrin-1 (netr: Sanskrit for “one who guides”) is a 75kD glycoprotein that is closely related to the laminin y domain and functions as a chemoattractive or chemorepulsive guidance cue in the central nervous system (CNS) during development (1-2). The protein is synthesized as a 604aa precursor that contains a 24aa signal sequence and a 580aa mature chain. Residues 46-283 constitute a laminin N-terminal domain (domain VI), while|285-453 make up three laminin-type epidermal growth factor-like domains. There is a final domain that runs from aa487-601 that qualifies as a Netrin- 1 like domain. It is also known as domain C in the context of C. elegans. There are four potential sites for N-linked glycosylation. Human Netrin-1 is 99% aa identical to mouse and rat Netrin-1. Netrin-1 is expressed in adult and embryonic tissues. In the adult, the protein is expressed in Schwann cells, oligodendrocytes and multiple neurons. In embryonic tissues, Netrin-1 is found in somatic mesoderm, heart, branchial pouch, and neuroepithelium. Netrin-1 is a secreted protein that, in addition to its involvement in outgrowth and migration orientation in the developing CNS, plays a significant role in the morphogenesis of endothelial cells and vascular smooth-muscle cells. It is also involved in the processes of cytoskeleton reorganization, angiogenesis, epithelial cell adhesion, and cell migration in the lungs, mammary gland, and pancreas (1, 3). Netrin-1 effects are controlled through different transmembrane receptors (2). Four of the receptors exist in the Unc-5 (Unc=uncontrolled behaviorally) family of proteins, and these include Unc5h1, Unc5h2, Unc5h3/RCM, and Unc5h4. There are also two receptors that belong to the UNC-40 family of molecules. The first is DCC (deleted in colorectal cancer), and the second is neogenin (newly-generated). UNC-5 receptors are noted to mediate the attraction response of axons and netrins. However, UNC-5 molecules, when co-expressed with DCC in the presence of Netrin-1, will mediate repulsion by varying cellular calcium levels (3). The adenosine A2b receptor may also be involved in chemoattraction, either by binding directly with Netrin-1 or by serving as a co-receptor for DCC. These receptors are known as dependence receptors because they depend on their ligand, in this case Netrin-1, for survival (4). Unbound, the receptors induce a specific death signal (4). It is the dysregulation of these receptor systems that may have important roles in tumor biology. DCC and UNC5 proteins make up a system for either initiating or inhibiting apoptosis (4), and it is now believed that Netrin-1 and its dependence receptors play a major role in tumor biology (4-6).||Source:|Recombinant corresponding to aa22-604 (HPGGGSGGGSGGGS) of human Netrin-1, fused with 6-His tag at N-terminal, expressed in mouse myeloma cell line, NS0.||Molecular Weight: |~67.5kD||Endotoxin Level:|≤1EU/1ug (LAL)||Biological Activity:|Measured by its binding ability in a functional ELISA. When recombinant rat UNC5H2 Fc Chimera is immobilized at 5ug/ml, recombinant human Netrin-1 binds with an apparent KD < 1nM.|Measured in a cell proliferation assay using RT4-D6P2T rat Schwann cells (ATCC: CRL-2768). The ED50 for this effect is typically 0.5-2ug/ml.||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

USBIo全称United States BIOLOGical，位于麻省斯万普斯科特(Swampscott, MA)。全美**的抗原抗体和生化试剂供应商，目前向全球六十余个国家的科研工作者提供约50,000种抗体、抗原和生化试剂，以质量**闻名。主要产品种类包括：抗体、生化试剂、基因克隆相关产品、培养基、生长因子等细胞因子、分子生物学试剂。美国US Biological公司是生物化学和生物学试剂的专业生产商，产品服务于免疫学，分子生物学，分子遗传学，体外诊断，组织培养等科研领域。产品精确度高，质量稳定，产品数量巨大，提供70,000多种抗体、抗原和生化试剂等。

Usbio的生物化学品，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。USBio产品从经济实惠的研究数量到更大的批量可用于工艺开发和生产。我们的客户包括位于美国和国外的大多数*的制药和生物技术公司。

USbiological公司致力于以价值，诚信和真正的个人购买体验降低研究成本。USbiological的生物化学，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。产品可以从负担得起的研究数量到大批量用于工艺开发和生产。

USBio（UnitedStatesBiological，或简称USBiological）位于麻省斯万普斯科特(Swampscott,MA)。作为全美最大的抗原抗体和生化试剂供应商，USBiological目前向全球六十余个国家的科研工作者提供约150,000种抗体、抗原和生化试剂，以质量卓越闻名。主要产品种类包括：抗体、生化试剂、基因克隆相关产品、培养基、生长因子等细胞因子、分子生物学试剂。美国USBiological的产品服务于免疫学，分子生物学，分子遗传学，体外诊断，组织培养等科研领域。USBiological一直恪守“为您的研究减少成本”原则，提供高纯度、高品质的产品，并在大多数生化试剂、生物与培养基产品线上保持最高的性价比。USBiological的产品被广泛用于各种科学研究领域，包括基因组研究、生物技术、药物研发和疾病诊断等。USBiological的产品从小型科研级到生物制品大规模级规格均有提供。众多全球顶ji的生物制药和生物技术公司都在使用USBiological的高品质产品。

usbio的生化试剂，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。

恭喜苏州蚂蚁淘获得usbio2020年授权书，成为usbio中国代理，购买usbio,请认准usbio代理-苏州蚂蚁淘！