
Recombinant Interferon Alpha (IFNa)
IFNA1; IFL; LeIF D; IFN; IFN-Alpha; IFNA13; IFN-A; IFNAP22; IFN-Alpha 1b; Interferon Alpha 1b; Interferon, Leukocytic; IFN, Leukocyte; Interferon alpha-D
- Product No.RPA033Rb01
- Organism SpeciesOryctolagus cuniculus (Rabbit) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- SourceProkaryotic expression
- HostE.coli
- Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
- Subcellular LocationSecreted
- Predicted Molecular Mass40.1kDa
- Accurate Molecular Mass43kDa(Analysis of differences refer to the manual)
- Residues & TagsSer1~Val93 with N-terminal His and GST Tag
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 80%
- Isoelectric Pointn/a
- ApplicationsPositive Control; Immunogen; SDS-PAGE; WB.If bio-activity of the protein is needed, please check active protein.
- DownloadInstruction Manual
- UOM10µg50µg200µg1mg5mg
- FOBUS$ 194 For more details, please contact local distributors!US$ 486 For more details, please contact local distributors!US$ 972 For more details, please contact local distributors!US$ 2916 For more details, please contact local distributors!US$ 7290 For more details, please contact local distributors!
SEQUENCE of the Recombinant Interferon Alpha (IFNa)
USAGE of the Recombinant Interferon Alpha (IFNa)
Reconstitute in PBS or others.
STORAGE of the Recombinant Interferon Alpha (IFNa)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Recombinant Interferon Alpha (IFNa)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
Polyacrylamide Gel Electrophoresis (PAGE) Experiment Service
INCREMENT SERVICES
Endotoxin Removal KitBCA Protein Quantification KitProtein Labeling Customized ServiceMolecular Mass Marker for ProteinRecombinant Protein Customized ServiceMonoclonal Antibody Customized ServicePolyclonal Antibody Customized ServiceProtein Activity Test Experiment ServiceImmunoprecipitation (IP) Experiment ServiceBufferEndotoxin Removal Kit IIReal Time PCR Experimental ServiceSpike RBD Protein (S-RBD)Protein GProtein A
Related products
Catalog No. | Organism species: Oryctolagus cuniculus (Rabbit) | Applications (RESEARCH USE ONLY!) |
RPA033Rb01 | Recombinant Interferon Alpha (IFNa) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA033Rb02 | Recombinant Interferon Alpha (IFNa) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA033Rb51 | Polyclonal Antibody to Interferon Alpha (IFNa) | WB; IHC; ICC; IP. |
LAA033Rb71 | Biotin-Linked Polyclonal Antibody to Interferon Alpha (IFNa) | WB; IHC; ICC. |
MAA033Rb21 | Monoclonal Antibody to Interferon Alpha (IFNa) | WB; IHC; ICC; IP. |
IEA033Rb | Instant ELISA Kit for Interferon Alpha (IFNa) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SEA033Rb | ELISA Kit for Interferon Alpha (IFNa) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA033Rb | CLIA Kit for Interferon Alpha (IFNa) | Chemiluminescent immunoassay for Antigen Detection. |
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各位业内前辈,我们正在考虑引进符合GMP认证标准的CHO细胞系,用于表达可做疫苗生产的重组蛋白类。目前已有符合标准信息的是Thermofisher的CHO-S悬浮培养细胞资料,希望能再多了解一些和这株细胞类似的其他公司符合GMP标准的生产株细胞做个比较。谢谢指教!
国内或者国外进口皆可!?看来你不缺钱啊!
这个问题问的过于笼统!
首先,蛋白表达与纯化包括很多种类型,比如原核蛋白表达,哺乳动物蛋白表达,酵母蛋白表达以及昆虫蛋白表达等等,而现在生物实验中常说的蛋白表达纯化通常是指利用大肠杆菌表达系统的原核蛋白表达,这种表达方式比较简单,普遍都可以做。但是如果是指很多种蛋白表达系统的话,可以做的单位就比较少了。
另外,蛋白的表达成功与否还需要取决于蛋白的性质,所以前期一定要问清楚!
事实上,现在多数药用级别白蛋白都是用血清生产的.
白蛋白的销售方向若是面向实验室,可采用重组质粒转到微生物发酵的方法生产,对土地面积的要求小,更集约,成本效率更高.
我想在体外培养的细胞中,加入PD-L1重组蛋白,激活PD-L1:PD-1通路,但我没能查到相关的文献,不清楚PD-L1重组蛋白的用量。请问各位有相关的经验吗?或者阅读过相关的文献?
而没答案
帮帮我
给我提供答案
考研细胞的题答案
基因敲除除可中止某一基因的表达外,还包括引入新基因及引入定点突变。既可以是用突变基因或其它基因敲除相应的正常基因,也可以用正常基因敲除相应的突变基因。 基因敲除是80年代后半期应用DNA同源重组原理发展起来的一门新技术。80年代初,胚胎干细胞(ES细胞)分离和体外培养的成功奠定了基因敲除的技术基础。1985年,首次证实的哺乳动物细胞中同源重组的存在奠定了基因敲除的理论基础。到1987年,Thompsson首次建立了完整的ES细胞基因敲除的小鼠模型。此后的几年中,基因敲除技术得到了进一步的发展和完善。
基因敲除的技术路线如下:
(1)构建重组基因载体﹔
(2)用电穿孔、显微注射等方法把重组DNA转入受体细胞核内﹔
(3)用选择培养基筛选已击中的细胞﹔
(4)将击中细胞转入胚胎使其生长成为转基因动物,对转基因动物进行形态观察及分子生物学检测。
基因敲除的靶细胞目前最常用的是小鼠ES细胞。基因敲除的技术路线虽不复杂,但由于高等真核细胞内外源DNA与靶细胞DNA序列自然发生同源重组的机率非常低,约为百万分之一,要把基因敲除成功的细胞筛选出来是一件非常困难的工作。因此,同源重组的筛选和检测就成了基因敲除技术所要解决的关键问题。目前已有多种筛

