Type
Recombinant
Description
Total321 AA.MW:36.3 kDa(calculated).UniProtKBacc.No.Q13296(Gly19–Phe93).C-terminallinker(2 extraAA),TEVsite(7 AA),Hu-IgG1fragment(Pro100-Lys330,231 AA)andC-terminalHis-tag(6 AA).ProteinidentityconfirmedbyLC-MS/MS.
AminoAcidSequence
GSGCPLLENVISKTINPQVSKTEYKELLQEFIDDNATTNAIDELKECFLNQTDETLSNVEVFMQLIYDSSLCDLFKLENLYFQGPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH
Source
HEK293
Purity
Purityasdeterminedbydensitometricimageanalysis:>95 %
SDS-PAGEGel
14 %SDS-PAGEseparationofHumanMammaglobinA(FcIgG):
1. M.W.Marker –14,21,31,45,66,97 kDa
2.Reducedandboiledsample,2.5 μg/lane
3.Non-reducedandnon-boiledsample,2.5 μg/lane
Endotoxin
<1.0 EU/μg
Formulation
Filtered(0.4μm)andlyophilizedfrom0.5mg/mlsolutioninphosphatebufferedsaline,5%w/vtrehalosepH7.4
Reconstitution
Adddeionizedwatertoprepareaworkingstocksolutionofapproximately0.5mg/mlandletthelyophilizedpelletdissolvecompletely.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.
Applications
Westernblotting,ELISA,Cellcultureand/oranimalstudies
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Storethelyophilizedproteinat-80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat-80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat-80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4°Cfor5days.
QualityControlTest
BCAtodeterminequantityoftheprotein.
SDSPAGEtodeterminepurityoftheprotein.
LALtodeterminequantityofendotoxin.
Note
Thisproductisintendedforresearchuseonly.