- Cis-diol specific, enriches heterogeneous sets of glycoproteins
- Unmasks glycoproteins from high abundance proteins, most notably albumin
- Disposable, no column regeneration or cross-contamination
- Efficient new surface technology ideal for proteomic applications
- Enriches and purifies glycoproteins from blood, serum, plasma, tissue or cell culture media.
- Removes > 90% of serum albumin (the non-glycosylated fraction)
- Sorbitol elution at pH 7.5 – 8.5
- Adaptable to a high-throughput 96-well format
Silica has been an industry standard as an advantageous matrix suitable for high performance liquid chromatography. With NuGel™, non-specific sites have been virtually eliminated making it an ideal support for affinity purification. Through a proprietary polymer coating, silica is crosslinked forming a reactive Poly-Epoxy functionality stable across a wide pH range (pH 2 to 10). From this foundational chemistry, all of the NuGel™ affinity products are derived.
The Phenyl Boronic Acid (PBA) ligand is immobilized through the NuGel™ poly-Epoxy linkage with attachment through the amino group. While various lectins bind to specific saccharide residues, the PBA ligand binds to the 1,2-cis-diol groups of biomolecules and enriches for heterogeneous sets of glycoproteins containing both N-linked and O-linked oligosaccharides. An easy and fast spin-filter format makes glycoprotein enrichment simple starting from 50µl serum, or 1-2 mg total protein.
Click Here For NuGel™ Glycoprotein Enrichment PBA Kit Product Sheet美国BSG（BiotechSupportGroup""LLC）公司是一家公司是一家全球知名的蛋白质组和基因组样本预处理的公司，是这个领域公认的领导者，该公司将其发明的专利表面化学技术应用生物学领域，比其他方法如亲合技术，苯酚法有绝对的优势。由于技术上的领先，使得BSG公司的产品广泛被美国国家基因组研究所，斯坦福大学等重要机构使用。
美国ＢＳＧ（Biotech Support Group,LLC）公司是一家公司是一家全球知名的蛋白质组和基因组样本预处理的公司，是这个领域公认的*，该公司将其发明的表面化学技术应用生物学领域，比其他方法如亲合技术，苯酚法有绝对的优势。由于技术上的，使得ＢＳＧ公司的产品广泛被美国国家基因组研究所，斯坦福大学等重要机构使用。（http://www.biotechsupportgroup。。ｃｏｍ/） 目前我司成功取得美国ＢＳＧ中国总代理，BSG各大产品均可在我司查询订购。 分子生物学 Ambion，Biosearch Technologies，Biotium，BPS bioscience，Dharmacon，Glen Research，HYQ ，Lucigen， lumiprobe，MO BIO，Molecular probes，NEB，Qiagen 细胞生物学 AddEXBIO，Cellgro，Echelon,Equitech，Gemini，Gibco，InvivoGen，Mlltenyi，Partec,Spherotech，Stemcell Abbiotec，Abcam，ABD SEROtec，Abnova，ADI，Antibodies-Online，AssayPro，Biorbyt，BioVision，BTI，Cellgro，CST，chromotek，Covance，Echelon，Endocrine Tech，Epitomics ，FD Neuro Technologies，Fitzgerald，GeneTex，GenWay，Gibco，Invitrogen，Invivogen，Jackson，Lifespan，LKT Labs，Miltenyi，Novex，Novus，Phoenix peptide，Pierece,，Plantmedia，Prospec，R&D，Spherotech，Stemcell，Trevigen，Santa cruz,，Separopore，USBIo，Vector Beckman coulter，BD，Biolegend，Affymetrix eBioscience，Tonbo A.G.Scientific，American Peptide，Apollo scientific，Cargille Lbs，Cayman，Nanocs，polysciences，Sigma，Tocris 诊断 Biocare，Biospacific,DIARECT，Fujirebio Diagnostics，Roche,Kamiya Epicentre，Ion Torrent，KAPA Biosystems 仪器/耗材 Applied Biosystems，Agilent，BD，Beckman coulter，Bio-Rad，GE，Perkin Elmer，Millipore，Parafilm，Uniequip
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